Ontology assay concept

catalase test

AssayMech:catalase-test

Microbial characterization

An ontology assay concept for testing catalase activity using hydrogen peroxide and visible gas formation.

Download source YAML · View source at this revision

Ontology assay concept. Any recorded method summary describes the concept's scope; it does not supply a complete executable laboratory protocol.

Record evidence

METPO ontology at commit 800c9d27913158efdf466edf2cd710dbf1ce893f — The selected class defines the named assay; its identity alone is not an organism-level positive or negative result.

Source reference · /graphs/0/nodes/1440 · Source licence: CC-BY-4.0

Interpretation and limitations

This is an assay concept, with no organism or strain result asserted.
METPO supplies a conceptual definition, not a complete executable protocol, validated threshold or performance estimate.

Ontology groundings

Assay identity, measured phenotype and tested predicate are distinct relations. A property grounding does not turn that property into an assay identity.

catalase test · METPO:1007080

Assay identity · Entity type: Class

Ontology source: metpo.json
Exact source revision: 800c9d27913158efdf466edf2cd710dbf1ce893f

METPO ontology at commit 800c9d27913158efdf466edf2cd710dbf1ce893f — The pinned node is the named assay parent, distinct from its positive and negative outcome classes.

Source reference · /graphs/0/nodes/1440 · Source licence: CC-BY-4.0

Method summary

Expose cells to hydrogen peroxide and observe visible bubbling.

METPO ontology at commit 800c9d27913158efdf466edf2cd710dbf1ce893f — The selected class defines the named assay; its identity alone is not an organism-level positive or negative result.

Source reference · /graphs/0/nodes/1440 · Source licence: CC-BY-4.0

Measurement and interpretation

Target: Catalase activity
Readout: Visible bubbling as a qualitative test response.
Unit: No unit recorded
Interpretation: A response must be interpreted under a separately specified protocol and controls; this record assigns no outcome to an organism.

METPO ontology at commit 800c9d27913158efdf466edf2cd710dbf1ce893f — The selected class defines the named assay; its identity alone is not an organism-level positive or negative result.

Source reference · /graphs/0/nodes/1440 · Source licence: CC-BY-4.0

Controls

No controls recorded. This is not a statement that controls are unnecessary.

Scoped CMM relationships

No CMM relationship recorded; no relationship is inferred from a shared assay name.

Research gaps

cmmmech: No source-backed correspondence between this assay concept and a specific CMM mechanism was established for this seed.
controls: The selected METPO definition does not establish an assay-specific positive/negative control protocol.
protocol_parameters: Reagent concentrations, incubation conditions, decision thresholds and performance characteristics require a separately sourced operational protocol.

Curation provenance

Recorded curation events
UTC timestamp	Action and curator	Summary
2026-11-09T02:38:32Z	CREATE · assay-stream-c	Curate initial sourced assay concept or scoped operational assay History source