{
  "id": "CofactorMech:cobalt",
  "name": "cobalt(2+)",
  "chemical_id": "CHEBI:48828",
  "categories": [
    "METAL_ION"
  ],
  "description": "cobalt(2+) is represented by the precise ChEBI entity CHEBI:48828. Cofactor roles are chemical-context annotations; ingredient links describe chemical supply and do not establish uptake, bioavailability or a requirement for every organism.",
  "evidence": [
    {
      "reference": "https://www.ebi.ac.uk/chebi/CHEBI:48828",
      "title": "ChEBI CHEBI:48828",
      "claim": "ChEBI assigns a cofactor, coenzyme or prosthetic-group role to CHEBI:48828.",
      "locator": "JSON Pointer /roles_classification",
      "source_license": "CC-BY-4.0"
    }
  ],
  "related_records": [
    {
      "corpus": "mediaingredientmech",
      "identifier": "CHEBI:53503",
      "relation": "SUPPLIED_BY",
      "basis": "The source is a chemically specified salt containing this metal in the +2 oxidation state; supply is chemical composition, not proof of uptake, cellular availability or enzyme occupancy.",
      "source_version": "0529fc6bc065bfa04aba0aed234f6b0cfad6a142",
      "source_path": "data/ingredients/mapped/Cobalt_chloride_hexahydrate.yaml",
      "source_url": "https://github.com/CultureBotAI/MediaIngredientMech/blob/0529fc6bc065bfa04aba0aed234f6b0cfad6a142/data/ingredients/mapped/Cobalt_chloride_hexahydrate.yaml",
      "status": "PROVISIONAL",
      "evidence": [
        {
          "reference": "https://github.com/CultureBotAI/MediaIngredientMech/blob/0529fc6bc065bfa04aba0aed234f6b0cfad6a142/data/ingredients/mapped/Cobalt_chloride_hexahydrate.yaml",
          "title": "MIM ingredient identity",
          "claim": "The cited ingredient is mapped to CHEBI:53503; this is a compound-identity assertion, not a bioavailability measurement.",
          "locator": "JSON Pointer /identifier",
          "source_license": "CC-BY-4.0"
        },
        {
          "reference": "https://www.ebi.ac.uk/chebi/CHEBI:53503",
          "title": "ChEBI CHEBI:53503",
          "claim": "The exact source salt or hydrate supplies the chemical component represented by CHEBI:35696; this is a composition relationship.",
          "locator": "JSON Pointer /ontology_relations",
          "source_license": "CC-BY-4.0"
        },
        {
          "reference": "https://www.ebi.ac.uk/chebi/CHEBI:35696",
          "title": "ChEBI CHEBI:35696",
          "claim": "The parent salt contains the metal in oxidation state +2; the resulting ion is CHEBI:48828.",
          "locator": "JSON Pointer /ontology_relations",
          "source_license": "CC-BY-4.0"
        }
      ]
    },
    {
      "corpus": "mediaingredientmech",
      "identifier": "CHEBI:35696",
      "relation": "SUPPLIED_BY",
      "basis": "The source is a chemically specified salt containing this metal in the +2 oxidation state; supply is chemical composition, not proof of uptake, cellular availability or enzyme occupancy.",
      "source_version": "0529fc6bc065bfa04aba0aed234f6b0cfad6a142",
      "source_path": "data/ingredients/mapped/Cocl2.yaml",
      "source_url": "https://github.com/CultureBotAI/MediaIngredientMech/blob/0529fc6bc065bfa04aba0aed234f6b0cfad6a142/data/ingredients/mapped/Cocl2.yaml",
      "status": "PROVISIONAL",
      "evidence": [
        {
          "reference": "https://github.com/CultureBotAI/MediaIngredientMech/blob/0529fc6bc065bfa04aba0aed234f6b0cfad6a142/data/ingredients/mapped/Cocl2.yaml",
          "title": "MIM ingredient identity",
          "claim": "The cited ingredient is mapped to CHEBI:35696; this is a compound-identity assertion, not a bioavailability measurement.",
          "locator": "JSON Pointer /identifier",
          "source_license": "CC-BY-4.0"
        },
        {
          "reference": "https://www.ebi.ac.uk/chebi/CHEBI:35696",
          "title": "ChEBI CHEBI:35696",
          "claim": "The source compound contains the metal in oxidation state +2, corresponding to CHEBI:48828; supply does not imply enzyme occupancy.",
          "locator": "JSON Pointer /ontology_relations",
          "source_license": "CC-BY-4.0"
        }
      ]
    },
    {
      "corpus": "mediaingredientmech",
      "identifier": "CHEBI:53503",
      "relation": "SUPPLIED_BY",
      "basis": "The source is a chemically specified salt containing this metal in the +2 oxidation state; supply is chemical composition, not proof of uptake, cellular availability or enzyme occupancy.",
      "source_version": "0529fc6bc065bfa04aba0aed234f6b0cfad6a142",
      "source_path": "data/ingredients/mapped/Cocl2_X_6_H2o.yaml",
      "source_url": "https://github.com/CultureBotAI/MediaIngredientMech/blob/0529fc6bc065bfa04aba0aed234f6b0cfad6a142/data/ingredients/mapped/Cocl2_X_6_H2o.yaml",
      "status": "PROVISIONAL",
      "evidence": [
        {
          "reference": "https://github.com/CultureBotAI/MediaIngredientMech/blob/0529fc6bc065bfa04aba0aed234f6b0cfad6a142/data/ingredients/mapped/Cocl2_X_6_H2o.yaml",
          "title": "MIM ingredient identity",
          "claim": "The cited ingredient is mapped to CHEBI:53503; this is a compound-identity assertion, not a bioavailability measurement.",
          "locator": "JSON Pointer /identifier",
          "source_license": "CC-BY-4.0"
        },
        {
          "reference": "https://www.ebi.ac.uk/chebi/CHEBI:53503",
          "title": "ChEBI CHEBI:53503",
          "claim": "The exact source salt or hydrate supplies the chemical component represented by CHEBI:35696; this is a composition relationship.",
          "locator": "JSON Pointer /ontology_relations",
          "source_license": "CC-BY-4.0"
        },
        {
          "reference": "https://www.ebi.ac.uk/chebi/CHEBI:35696",
          "title": "ChEBI CHEBI:35696",
          "claim": "The parent salt contains the metal in oxidation state +2; the resulting ion is CHEBI:48828.",
          "locator": "JSON Pointer /ontology_relations",
          "source_license": "CC-BY-4.0"
        }
      ]
    },
    {
      "corpus": "proteintraitsmech",
      "identifier": "proteintraitsmech:COFACTOR_REQ_CHEBI_48828",
      "relation": "REQUIRED_BY",
      "basis": "Conceptual requirement coverage: this PTM trait defines the requirement for the same ChEBI chemical. It is not an organism-level or enzyme-level measurement.",
      "source_version": "8d08b33cfbee1e9d1c2fdb2211b82b49dd0c8b1f",
      "source_path": "data/traits/function/cofactor_requirement/chebi/cobalt-2-chebi48828.yaml",
      "source_url": "https://github.com/CultureBotAI/proteintraitsmech/blob/8d08b33cfbee1e9d1c2fdb2211b82b49dd0c8b1f/data/traits/function/cofactor_requirement/chebi/cobalt-2-chebi48828.yaml",
      "status": "VERIFIED",
      "evidence": [
        {
          "reference": "https://github.com/CultureBotAI/proteintraitsmech/blob/8d08b33cfbee1e9d1c2fdb2211b82b49dd0c8b1f/data/traits/function/cofactor_requirement/chebi/cobalt-2-chebi48828.yaml",
          "title": "PTM cofactor-requirement concept",
          "claim": "The target is a CLASS trait with an explicit cross-reference to this chemical, rather than a measured protein observation.",
          "locator": "JSON Pointer /xrefs",
          "source_license": "CC-BY-4.0"
        }
      ]
    },
    {
      "corpus": "pathwaymech",
      "identifier": "MetaCyc:PWY-8130",
      "relation": "USED_BY",
      "basis": "PathwayMech edge ald2-alternative-cobalt records UniProtKB:Q2RXI1 has_cofactor CHEBI:48828. This is a cross-reference to the source-scoped reaction or cofactor annotation, not proof of complete pathway flux. The cobalt evidence is an alternative in vitro reconstitution of recombinant Rhodospirillum rubrum Ald2, not established physiological occupancy or simultaneous cobalt/nickel dependence.",
      "source_version": "9584009d49579267233124f4a1057b9425a007d9",
      "source_path": "data/pathways/5-prime-deoxyadenosine-degradation-i.yaml",
      "source_url": "https://github.com/CultureBotAI/PathwayMech/blob/9584009d49579267233124f4a1057b9425a007d9/data/pathways/5-prime-deoxyadenosine-degradation-i.yaml",
      "status": "VERIFIED",
      "evidence": [
        {
          "reference": "https://github.com/CultureBotAI/PathwayMech/blob/9584009d49579267233124f4a1057b9425a007d9/data/pathways/5-prime-deoxyadenosine-degradation-i.yaml",
          "title": "PathwayMech reaction or cofactor edge",
          "claim": "PathwayMech edge ald2-alternative-cobalt records UniProtKB:Q2RXI1 has_cofactor CHEBI:48828. This is a cross-reference to the source-scoped reaction or cofactor annotation, not proof of complete pathway flux. The cobalt evidence is an alternative in vitro reconstitution of recombinant Rhodospirillum rubrum Ald2, not established physiological occupancy or simultaneous cobalt/nickel dependence.",
          "locator": "JSON Pointer /mechanistic_edges/19",
          "source_license": "CC-BY-4.0"
        }
      ]
    }
  ],
  "research_gaps": [
    {
      "corpus": "proteintraitsmech",
      "reason": "The curated PTM link covers a cofactor-requirement concept only. A separately sourced, named enzyme exemplar requiring this exact chemical has not been curated in this seed record."
    }
  ],
  "curation_history": [
    {
      "timestamp": "2026-11-09T01:42:27Z",
      "curator": "cofactor-stream-c",
      "action": "CREATE",
      "summary": "Curate initial source-backed cofactor and scoped cross-corpus links",
      "history_file": "history/records/cobalt/2026-11-09T014227Z-cofactor-stream-c-74be1c.yaml"
    }
  ]
}