← Back to Communities

ANME/SRB Anaerobic Methanotrophic Syntrophic Consortia

Community ID CommunityMech:000276
Ecological State STABLE
Environment marine sediment
ENVO:03000033

Marine syntrophic consortia of anaerobic methanotrophic archaea (ANME) and sulfate-reducing bacteria (SRB) that couple the anaerobic oxidation of methane to sulfate reduction in marine sediments. Sediment-free enrichment cultures of three consortium types (ANME-1/Desulfofervidus, ANME-2a/Seep-SRB1, and ANME-2a+2c/Seep-SRB1+2) exhibited high dry conductance close to that of electrogenic biofilms. Cyclic voltammetry revealed redox activity and generator-collector measurements showed electron transport over micrometer-scale distances, establishing that the symbiosis uses redox conduction (consistent with multiheme cytochrome c) for direct interspecies electron transport between the archaeal and bacterial partners.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
ANME-1 (anaerobic methanotrophic archaea, clade 1) NCBITaxon:588814
SYNTROPHIC_PARTNER ELECTRON_DONOR
N/A
  • PMID:40845095 - SUPPORT (IN_VITRO)
    "Cyclic voltammetry revealed redox activity centered at 28 ± 11, 94 ± 6, and 24 ± 7 millivolts for ANME-1/Desulfofervidus, ANME-2a/Seep-SRB1, and ANME-2a+2c/Seep-SRB1+2 consortia, respectively."
Desulfofervidus (sulfate-reducing bacterial partner of ANME-1) NCBITaxon:1902583
SYNTROPHIC_PARTNER ELECTRON_ACCEPTOR
N/A
  • PMID:40845095 - SUPPORT (IN_VITRO)
    "Cyclic voltammetry revealed redox activity centered at 28 ± 11, 94 ± 6, and 24 ± 7 millivolts for ANME-1/Desulfofervidus, ANME-2a/Seep-SRB1, and ANME-2a+2c/Seep-SRB1+2 consortia, respectively."
ANME-2a (anaerobic methanotrophic archaea, clade 2a) NCBITaxon:588816
SYNTROPHIC_PARTNER ELECTRON_DONOR
N/A
  • PMID:40845095 - SUPPORT (IN_VITRO)
    "Cyclic voltammetry revealed redox activity centered at 28 ± 11, 94 ± 6, and 24 ± 7 millivolts for ANME-1/Desulfofervidus, ANME-2a/Seep-SRB1, and ANME-2a+2c/Seep-SRB1+2 consortia, respectively."
ANME-2c (anaerobic methanotrophic archaea, clade 2c) NCBITaxon:3386252
SYNTROPHIC_PARTNER ELECTRON_DONOR
N/A
  • PMID:40845095 - SUPPORT (IN_VITRO)
    "Cyclic voltammetry revealed redox activity centered at 28 ± 11, 94 ± 6, and 24 ± 7 millivolts for ANME-1/Desulfofervidus, ANME-2a/Seep-SRB1, and ANME-2a+2c/Seep-SRB1+2 consortia, respectively."
Seep-SRB1 (sulfate-reducing bacterial partner of ANME-2a) NCBITaxon:213119
SYNTROPHIC_PARTNER ELECTRON_ACCEPTOR
N/A
  • PMID:40845095 - SUPPORT (IN_VITRO)
    "Cyclic voltammetry revealed redox activity centered at 28 ± 11, 94 ± 6, and 24 ± 7 millivolts for ANME-1/Desulfofervidus, ANME-2a/Seep-SRB1, and ANME-2a+2c/Seep-SRB1+2 consortia, respectively."
Seep-SRB2 (additional sulfate-reducing bacterial partner) NCBITaxon:213118
SYNTROPHIC_PARTNER ELECTRON_ACCEPTOR
N/A
  • PMID:40845095 - SUPPORT (IN_VITRO)
    "Cyclic voltammetry revealed redox activity centered at 28 ± 11, 94 ± 6, and 24 ± 7 millivolts for ANME-1/Desulfofervidus, ANME-2a/Seep-SRB1, and ANME-2a+2c/Seep-SRB1+2 consortia, respectively."

Ecological Interactions

Ecological interaction network for ANME/SRB Anaerobic Methanotrophic Syntrophic Consortia Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

ANME-1/Desulfofervidus syntrophy via direct interspecies electron transport

SYNTROPHY

Source Taxon: ANME-1

Target Taxon: Desulfofervidus

Evidence

  • PMID:40845095 - SUPPORT (IN_VITRO)
    "Anaerobic methanotrophic archaea (ANME) and sulfate-reducing bacteria (SRB) form syntrophic partnerships in marine sediments to consume greenhouse gas methane."
  • PMID:40845095 - SUPPORT (IN_VITRO)
    "Collectively, our results establish that marine ANME/SRB symbiosis uses redox conduction, consistent with multiheme cytochrome c, for direct interspecies electron transport."

ANME-2a/Seep-SRB1 syntrophy via direct interspecies electron transport

SYNTROPHY

Source Taxon: ANME-2a

Target Taxon: Seep-SRB1

Evidence

  • PMID:40845095 - SUPPORT (IN_VITRO)
    "Generator-collector measurements further demonstrated that these redox components facilitate electron transport over micrometer-scale distances, sufficient to link archaeal and bacterial partners."

ANME-2a+2c/Seep-SRB1+2 syntrophy via direct interspecies electron transport

SYNTROPHY

Source Taxon: ANME-2c

Target Taxon: Seep-SRB2

Evidence

  • PMID:40845095 - SUPPORT (IN_VITRO)
    "Diverse ANME/SRB consortia exhibited high dry conductance close to electrogenic biofilms."

Growth Media

anoxic artificial seawater medium (defined HEPES-buffered marine mineral medium)

pH 7.3
Atmosphere ANAEROBIC

Preparation notes: Per liter Milli-Q water: 5.67 g MgCl2·6H2O, 0.22 g CaCl2·2H2O, 26.37 g NaCl, 0.6 g KCl, 1.5 g Na2SO4, 0.174 g K2HPO4, 0.107 g NH4Cl, 25 mM HEPES; trace elements added, no vitamins. ANME-1/Desulfofervidus grown at 50°C, ANME-2a/Seep-SRB1 at 10°C, ANME-2a+2c/Seep-SRB1+2 at 20°C.

Evidence

PMID:40845095

"grown at 50°C, ANME-2a partnered with Seep-SRB1 (ANME-2a/Seep-SRB1 consortia) grown at 10°C, and ANME-2a and ANME-2c partnered with Seep-SRB1 and Seep-SRB2 grown at 20°C"

PMID:40845095

"the pH of the medium was adjusted to 7.3, filter sterilized through a 0.22-μm filter, and sparged anoxic using N 2 gas for at least 20 min"