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Acetylene-Fueled Trichloroethene Dechlorination Groundwater Enrichment

Community ID CommunityMech:000238
Ecological State ENGINEERED
Environment contaminated groundwater enrichment culture
ENVO:01001405

A microbial community enriched from TCE/PCE-contaminated groundwater and amended with acetylene (C2H2) as the sole electron donor and organic carbon source. The stable enrichment performs reductive dechlorination of trichloroethene (TCE) and tetrachloroethene (PCE), coupling acetylenotrophy to organohalide respiration. Metagenomic analysis of the stable community identified a novel anaerobic acetylenotroph in the phylum Actinobacteria, expanding the known diversity of this metabolism beyond previously documented Pelobacter SFB93 + Dehalococcoides mccartyi laboratory cocultures.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
novel anaerobic acetylenotroph (Actinobacteria) NCBITaxon:201174
PRIMARY_DEGRADER CROSS_FEEDER
N/A
  • PMID:33531396 - SUPPORT (IN_VITRO)
    "A novel anaerobic acetylenotroph in the phylum Actinobacteria was identified using metagenomic analysis"
reductive dechlorinating community members NCBITaxon:2
PRIMARY_DEGRADER
N/A
  • PMID:33531396 - SUPPORT (IN_VITRO)
    "TCE and perchloroethene (PCE) reductive dechlorination by a microbial community enriched from contaminated groundwater"

Ecological Interactions

Ecological interaction network for Acetylene-Fueled Trichloroethene Dechlorination Groundwater Enrichment Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Acetylenotrophy Provides Reductant for Dechlorination

SYNTROPHY

Source Taxon: novel anaerobic acetylenotroph (Actinobacteria)

Target Taxon: reductive dechlorinating community members

Metabolites: acetylene (CHEBI:27518), trichloroethene (CHEBI:16602), tetrachloroethene (CHEBI:17300)

Biological Processes:

Evidence

  • PMID:33531396 - SUPPORT (IN_VITRO)
    "the coupling of acetylenotrophy and reductive dechlorination can occur in the environment with native bacteria"

Acetylene as Sole Electron Donor and Carbon Source

CROSS_FEEDING

Metabolites: acetylene (CHEBI:27518)

Evidence

  • PMID:33531396 - SUPPORT (IN_VITRO)
    "amended with C2H2 as the sole electron donor and organic carbon source"

External Resources

Name Repository Resource ID
Primary publication for the acetylene TCE-dechlorination community
PubMed record for the Gushgari-Doyle et al. 2021 mBio paper on acetylene-fueled TCE reductive dechlorination in a groundwater enrichment culture. 		OTHER PMID:33531396
  • PMID:33531396 - SUPPORT (IN_VITRO)
    "Acetylene-Fueled Trichloroethene Reductive Dechlorination in a Groundwater Enrichment Culture"
DOI landing page
DOI link to the mBio paper. 		OTHER doi:10.1128/mBio.02724-20

Environmental Factors

Factor Value Unit
Acetylene source C2H2 supplied as sole electron donor and carbon source N/A
  • PMID:33531396 - SUPPORT (IN_VITRO)
    "acetylene (C2H2) is a product of abiotic degradation of trichloroethene (TCE) catalyzed by in situ minerals"
Chlorinated electron acceptors TCE and PCE N/A
  • PMID:33531396 - SUPPORT (IN_VITRO)
    "TCE and perchloroethene (PCE) reductive dechlorination"