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At-RSPHERE SynCom

Ecological State ENGINEERED
Environment rhizosphere
ENVO:00005801
Source File At_RSPHERE_SynCom.yaml

A synthetic bacterial community derived from the Arabidopsis root microbiota culture collection (At-RSPHERE), representing 16 bacterial families isolated from Arabidopsis thaliana roots grown in natural soil. This community was established to study plant-microbe interactions and their effects on plant productivity and carbon cycling in the rhizosphere. The consortium members were selected to represent a broad taxonomic range covering the major bacterial phyla (Proteobacteria, Actinobacteria, and Bacteroidetes) found in the Arabidopsis root microbiome. Assembly sizes vary from 5-20 members depending on experimental design, enabling reductionist studies of root microbiota assembly and function.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Arabidopsis thaliana NCBITaxon:3702
PRIMARY_PRODUCER
N/A
Pseudomonas sp. NCBITaxon:306
SYNTROPHIC_PARTNER
DOMINANT
Variovorax sp. NCBITaxon:1871043
SYNTROPHIC_PARTNER
DOMINANT
Rhodococcus sp. NCBITaxon:1827
SYNTROPHIC_PARTNER
COMMON
Streptomyces sp. NCBITaxon:1931
SYNTROPHIC_PARTNER
COMMON
Flavobacterium sp. NCBITaxon:239
SYNTROPHIC_PARTNER
COMMON

Ecological Interactions

Plant-Microbe Mutualism

MUTUALISM

Source Taxon: Pseudomonas sp.

Biological Processes:

Evidence

  • doi:10.1038/nature16192 - SUPPORT (IN_VITRO)
    "Using defined bacterial communities and a gnotobiotic Arabidopsis plant system we show that the isolates form assemblies resembling natural microbiota on their cognate host organs, but are also capable of ectopic leaf or root colonization"

Carbon Cycling and Nutrient Exchange

MUTUALISM

Source Taxon: Variovorax sp.

Biological Processes:

Evidence

  • doi:10.1038/nature16192 - SUPPORT (IN_VITRO)
    "We established Arabidopsis leaf- and root-derived microbiota culture collections representing the majority of bacterial species that are reproducibly detectable by culture-independent community sequencing"

Root Colonization and Microbiota Assembly

MUTUALISM

Source Taxon: Rhodococcus sp.

Biological Processes:

Evidence

  • doi:10.1038/nature16192 - SUPPORT (IN_VITRO)
    "Using defined bacterial communities and a gnotobiotic Arabidopsis plant system we show that the isolates form assemblies resembling natural microbiota on their cognate host organs, but are also capable of ectopic leaf or root colonization"

Environmental Factors

Factor Value Unit
Bacterial diversity 16 families N/A
Assembly size 5-20 members N/A
Taxonomic coverage multi-phylum N/A
Functional capacity carbon cycling and plant productivity N/A
Application plant microbiome research N/A