Caldibacillus-Clostridium Aerotolerant Cellulose Coculture

Community ID CommunityMech:000179

Ecological State ENGINEERED

Environment aerobic cellulose-fed laboratory coculture
ENVO:01001405

A designed two-member thermophilic coculture for aerotolerant cellulose conversion. The community pairs the cellulolytic strict anaerobe Clostridium thermocellum with the noncellulolytic facultative anaerobe Caldibacillus debilis GB1. C. debilis GB1 provides respiratory protection under oxidative conditions, enabling synergistic cellulose utilization and ethanogenic biofuel production under an aerobic atmosphere.

Taxonomy

Taxon	Ontology ID	Functional Roles	Abundance
Clostridium thermocellum	NCBITaxon:1515	PRIMARY_DEGRADER SECONDARY_FERMENTER	N/A
doi:10.1128/AEM.00735-15 - SUPPORT (IN_VITRO) "included a Clostridium thermocellum strain"
Caldibacillus debilis GB1	NCBITaxon:301148	CROSS_FEEDER SECONDARY_FERMENTER	N/A
doi:10.1128/AEM.00735-15 - SUPPORT (IN_VITRO) "noncellulolytic facultative anaerobe C. debilis GB1"

Ecological Interactions

Taxon

Cross-feeding

Mutualism

Syntrophy

Competition

Commensalism

Niche partitioning

Colonization facilitation

Strain competition

Predation

Respiratory Protection of C. thermocellum

COMMENSALISM

Source Taxon: Caldibacillus debilis GB1

Target Taxon: Clostridium thermocellum

Metabolites: dioxygen (CHEBI:15379), cellulose (CHEBI:18246)

Biological Processes:

interspecies interaction between organisms (GO:0044419)
cellulose catabolic process (GO:0030245)

Evidence

doi:10.1128/AEM.00735-15 - SUPPORT (IN_VITRO)

"C. debilis GB1 provided respiratory protection for C. thermocellum"
doi:10.1128/AEM.00735-15 - SUPPORT (IN_VITRO)

"synergistic utilization of cellulose even under an aerobic atmosphere"

Aerotolerant Ethanogenic Cellulose Conversion

CROSS_FEEDING

Source Taxon: Clostridium thermocellum

Target Taxon: Caldibacillus debilis GB1

Metabolites: cellulose (CHEBI:18246), ethanol (CHEBI:16236)

Biological Processes:

cellulose catabolic process (GO:0030245)
fermentation (GO:0006113)

Evidence

doi:10.1128/AEM.00735-15 - SUPPORT (IN_VITRO)

"aerotolerant ethanogenic biofuel production from cellulose"
doi:10.1128/AEM.00735-15 - SUPPORT (IN_VITRO)

"synergistic utilization of cellulose even under an aerobic atmosphere"

Oxidative Shift Toward Lactate Production

CROSS_FEEDING

Source Taxon: Caldibacillus debilis GB1

Metabolites: lactate (CHEBI:24996)

Biological Processes:

fermentation (GO:0006113)

Evidence

doi:10.1128/AEM.00735-15 - SUPPORT (IN_VITRO)

"including an increase in lactate production"

External Resources

Name	Repository	Resource ID
Exact-system primary publication - C. debilis GB1 and C. thermocellum coculture Primary publication describing the designed aerotolerant cellulose-degrading coculture with C. debilis GB1 and C. thermocellum.	OTHER	doi:10.1128/AEM.00735-15
doi:10.1128/AEM.00735-15 - SUPPORT (IN_VITRO) "aerotolerant ethanogenic biofuel production from cellulose"

Environmental Factors

Factor	Value	Unit
Cellulose substrate	cellulose	N/A
doi:10.1128/AEM.00735-15 - SUPPORT (IN_VITRO) "biofuel production from cellulose"
Aerobic atmosphere	oxygen exposure	N/A
doi:10.1128/AEM.00735-15 - SUPPORT (IN_VITRO) "even under an aerobic atmosphere"

Growth Media

Cellulose-fed aerotolerant coculture assay

Atmosphere FACULTATIVE_AEROBIC

Composition

Component	Concentration	Unit	CHEBI
cellulose	N/A	N/A	CHEBI:18246

Preparation notes: The abstract supports cellulose-fed aerotolerant coculture assays; exact medium formulation and temperature details are not asserted in this record.

Evidence

doi:10.1128/AEM.00735-15

"biofuel production from cellulose"