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Chlamydomonas-Bacterial Hydrogen Production Consortium

Community ID CommunityMech:000014
Ecological State ENGINEERED
Environment laboratory bioreactor
ENVO:01001405

A synthetic algae-bacteria consortium for sustainable hydrogen production, composed of the green alga Chlamydomonas reinhardtii and three bacterial species: Microbacterium forte sp. nov., Bacillus cereus, and Stenotrophomonas goyi sp. nov. This mutualistic association produces up to 313 mL H2/L over 17 days in high-cell density cultures. M. forte is the primary bacterial species responsible for improving algal hydrogen production, while the complete bacterial community enhances and sustains photobiological H2 generation through metabolic cooperation.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Chlamydomonas reinhardtii NCBITaxon:3055
PRIMARY_PRODUCER
DOMINANT
Microbacterium forte NCBITaxon:2982533
SYNTROPHIC_PARTNER
ABUNDANT
Bacillus cereus NCBITaxon:1396
SYNTROPHIC_PARTNER
COMMON
Stenotrophomonas goyi NCBITaxon:3050471
SYNTROPHIC_PARTNER
COMMON

Ecological Interactions

Ecological interaction network for Chlamydomonas-Bacterial Hydrogen Production Consortium Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism

Photosynthetic Hydrogen Production

COMMENSALISM

Source Taxon: Chlamydomonas reinhardtii

Metabolites: hydrogen (CHEBI:49637), oxygen (CHEBI:15379)

Biological Processes:

Downstream Effects:
→ Bacterial Enhancement of Algal H2 Production

Evidence

  • PMID:38159768 - SUPPORT (IN_VITRO)
    "Still, under non‑hydrogen producing conditions (aerobiosis) the Chlamydomonas-M"

Bacterial Enhancement of Algal H2 Production

MUTUALISM

Source Taxon: Microbacterium forte

Target Taxon: Chlamydomonas reinhardtii

Metabolites: mannitol (CHEBI:16899)

Biological Processes:

Evidence

  • PMID:38159768 - SUPPORT (IN_VITRO)
    "When incubated in mannitol- and yeast extract-containing medium, this bacterial community can promote and sustain algal hydrogen production up to 313 mL H2·L-1 for 17 days and 163.5 mL H2·L-1 for 25 days in high-cell (76.7 μg·mL-1 of initial chlorophyll) and low-cell density (10 μg·mL-1 of initial chlorophyll) algal cultures, respectively"

Multispecies Bacterial Consortium Supporting H2 Production

MUTUALISM

Source Taxon: Bacillus cereus

Target Taxon: Stenotrophomonas goyi

Metabolites: oxygen (CHEBI:15379), hydrogen (CHEBI:49637)

Biological Processes:

Evidence

  • PMID:38159768 - SUPPORT (IN_VITRO)
    "A naturally occurring multispecies bacterial community composed of Bacillus cereus and two novel bacteria (Microbacterium forte sp. nov. and Stenotrop"
  • PMID:38159768 - SUPPORT (IN_VITRO)
    "When incubated in mannitol- and yeast extract-containing medium, this bacterial community can promote and sustain algal hydrogen production up to 313 mL H2·L-1 for 17 days and 163.5 mL H2·L-1 for 25 days in high-cell (76.7 μg·mL-1 of initial chlorophyll) and low-cell density (10 μg·mL-1 of initial chlorophyll) algal cultures, respectively"

Environmental Factors

Factor Value Unit
Light continuous illumination N/A
Cell density 76.7 μg/mL chlorophyll (high) or 10 μg/mL (low) N/A
Medium composition mannitol and yeast extract N/A
Anaerobic conditions required for H2 production N/A

Growth Media

TAP medium with mannitol and yeast extract for hydrogen production (CultureMech:000139)

View in CultureMech →

pH 7.3
Temperature 25 degrees Celsius
Atmosphere FACULTATIVE_ANAEROBIC

Composition

Component Concentration Unit CHEBI
Tris base (buffer) 20 mM CHEBI:9754
Acetate (carbon source) 17 mM CHEBI:30089
Mannitol 20 mM CHEBI:16899
Yeast extract 0.1 percent (w/v) CHEBI:89981
Ammonium chloride (nitrogen source) 7.5 mM CHEBI:31206
Potassium phosphate 1.0 mM CHEBI:131527
Magnesium sulfate 0.4 mM CHEBI:32599
Calcium chloride 0.34 mM CHEBI:3312
See source for composition variable VARIABLE N/A

Preparation notes: Medium prepared for sustained algal-bacterial hydrogen production. Mannitol and yeast extract are added to support bacterial growth and enhance H2 production. Cultures start under aerobic conditions and transition to anaerobic conditions for hydrogen production. Sulfur deprivation may be used to induce anaerobiosis and activate hydrogenase in Chlamydomonas. High-cell density cultures (76.7 μg/mL chlorophyll) produce 313 mL H2/L over 17 days, while low-cell density cultures (10 μg/mL chlorophyll) produce 163.5 mL H2/L over 25 days.

Evidence

PMID:38159768

"When incubated in mannitol- and yeast extract-containing medium, this bacterial community can promote and sustain algal hydrogen production up to 313 mL H2·L-1 for 17 days and 163.5 mL H2·L-1 for 25 days in high-cell (76.7 μg·mL-1 of initial chlorophyll) and low-cell density (10 μg·mL-1 of initial chlorophyll) algal cultures, respectively"