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Clostridium cellulovorans-Rhodopseudomonas palustris Cellulose Biohydrogen Coculture

Community ID CommunityMech:000264
Ecological State ENGINEERED
Environment anaerobic illuminated laboratory serum-bottle culture
ENVO:01001405

A defined two-member cellulose-to-biohydrogen coculture combining the cellulolytic dark-fermentative bacterium Clostridium cellulovorans 743B with the purple nonsulfur photosynthetic bacterium Rhodopseudomonas palustris CGA009. The coculture links cellulose degradation, volatile-fatty-acid exchange, pH stabilization, and photofermentative hydrogen production. It is DOE-relevant as a genetically tractable model for lignocellulosic bioenergy, integrated dark/photo fermentation, and mechanistic design of biohydrogen consortia.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Clostridium cellulovorans 743B NCBITaxon:573061
PRIMARY_DEGRADER CROSS_FEEDER
ABUNDANT
  • doi:10.1128/AEM.00789-16 - SUPPORT (IN_VITRO)
    "artificial coculture of Clostridium cellulovorans 743B and Rhodopseudomonas palustris CGA009"
  • doi:10.1128/AEM.00789-16 - SUPPORT (IN_VITRO)
    "cellulosomal genes in C. cellulovorans were upregulated"
Rhodopseudomonas palustris CGA009 NCBITaxon:258594
CROSS_FEEDER PRIMARY_PRODUCER
ABUNDANT

Ecological Interactions

Ecological interaction network for Clostridium cellulovorans-Rhodopseudomonas palustris Cellulose Biohydrogen Coculture Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

VFA Cross-Feeding and pH Stabilization

CROSS_FEEDING

Source Taxon: Clostridium cellulovorans 743B

Target Taxon: Rhodopseudomonas palustris CGA009

Metabolites: cellulose (CHEBI:18246), acetate (CHEBI:30089)

Biological Processes:

Evidence

  • doi:10.1128/AEM.00789-16 - SUPPORT (IN_VITRO)
    "genes related to biosynthesis of volatile fatty acids (VFAs) in C. cellulovorans were upregulated"
  • doi:10.1128/AEM.00789-16 - SUPPORT (IN_VITRO)
    "a gene that mediates organic compound catabolism in R. palustris was also upregulated"

Integrated Dark and Photo Fermentation for Hydrogen

SYNTROPHY

Metabolites: dihydrogen (CHEBI:18276), cellulose (CHEBI:18246)

Biological Processes:

Evidence

  • doi:10.1128/AEM.00789-16 - SUPPORT (IN_VITRO)
    "consistent with the physiological observations of enhanced H 2 production and cellulose degradation"
  • doi:10.1128/AEM.00789-16 - SUPPORT (IN_VITRO)
    "the enhanced H 2 yield was contributed mainly by R. palustris"

Transcriptomic Division of Labor

CROSS_FEEDING

Biological Processes:

Evidence

  • doi:10.1128/AEM.00789-16 - SUPPORT (IN_VITRO)
    "nitrogen fixation genes in R. palustris and the cellulosomal genes in C. cellulovorans were upregulated"

Associated Datasets

Dataset Type Repository Accession
Cellulose-concentration physiology publication for C. cellulovorans-R. palustris coculture
Primary physiology paper establishing the exact C. cellulovorans 743B and R. palustris CGA009 cellulose-fed biohydrogen coculture. 		PHENOTYPE OTHER doi:10.1016/j.ijhydene.2015.05.135
Transcriptomic publication for the same C. cellulovorans-R. palustris coculture
Follow-up transcriptomics paper using the same exact C. cellulovorans 743B and R. palustris CGA009 cellulose-grown coculture. 		METATRANSCRIPTOME OTHER doi:10.1128/AEM.00789-16

Environmental Factors

Factor Value Unit
Renewable cellulose substrate cellulose-grown coculture N/A
Coculture VFA gradient VFA concentration gradient N/A

Growth Media

Cellulose-fed dark/photo-fermentation defined medium

Atmosphere ANAEROBIC

Composition

Component Concentration Unit CHEBI
Cellulose N/A N/A CHEBI:18246
Acetate N/A N/A CHEBI:30089

Preparation notes: Detailed medium formulation is not asserted here because the validator-visible source text is abstract-only. The record curates cellulose and representative volatile-fatty-acid exchange supported by the abstract.

Evidence

doi:10.1128/AEM.00789-16

"biohydrogen production using renewable cellulose"

doi:10.1128/AEM.00789-16

"genes related to biosynthesis of volatile fatty acids (VFAs) in C. cellulovorans were upregulated"