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Clostridium-Thermoanaerobacterium JN4-GD17 Cellulosic Biofuel Coculture

Community ID CommunityMech:000180
Ecological State ENGINEERED
Environment anaerobic cellulose-fed laboratory coculture
ENVO:01001405

A thermophilic two-member cellulosic biofuel coculture composed of Clostridium thermocellum JN4 and Thermoanaerobacterium thermosaccharolyticum GD17. The system improves overall cellulosic biofuel production efficiency relative to C. thermocellum alone, but later mechanistic work indicates that GD17 also competitively hampers C. thermocellum JN4 growth while using glucose and cellobiose released during cellulose degradation.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Clostridium thermocellum JN4 NCBITaxon:1515
PRIMARY_DEGRADER SECONDARY_FERMENTER
N/A
  • PMID:31551972 - SUPPORT (IN_VITRO)
    "Clostridium thermocellum JN4"
Thermoanaerobacterium thermosaccharolyticum GD17 NCBITaxon:1517
SECONDARY_FERMENTER CROSS_FEEDER
N/A
  • PMID:31551972 - SUPPORT (IN_VITRO)
    "Thermoanaerobacterium thermosaccharolyticum GD17"

Ecological Interactions

Ecological interaction network for Clostridium-Thermoanaerobacterium JN4-GD17 Cellulosic Biofuel Coculture Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Cellulosic Biofuel Efficiency Enhancement

CROSS_FEEDING

Source Taxon: Clostridium thermocellum JN4

Target Taxon: Thermoanaerobacterium thermosaccharolyticum GD17

Metabolites: cellulose (CHEBI:18246)

Biological Processes:

Evidence

  • PMID:31551972 - SUPPORT (IN_VITRO)
    "coculture significantly improves overall cellulosic biofuel production efficiency"

GD17 Competitive Conversion of Released Sugars

COMPETITION

Source Taxon: Thermoanaerobacterium thermosaccharolyticum GD17

Target Taxon: Clostridium thermocellum JN4

Metabolites: cellobiose (CHEBI:17057), glucose (CHEBI:17234)

Biological Processes:

Evidence

  • PMID:31551972 - SUPPORT (IN_VITRO)
    "strong competitive metabolic advantage of T. thermosaccharolyticum GD17 over C. thermocellum JN4"
  • PMID:31551972 - SUPPORT (IN_VITRO)
    "conversion of glucose or cellobiose into final products"

GD17 Growth Inhibition of JN4

COMPETITION

Source Taxon: Thermoanaerobacterium thermosaccharolyticum GD17

Target Taxon: Clostridium thermocellum JN4

Biological Processes:

  • interspecies interaction between organisms (GO:0044419)

Evidence

  • PMID:31551972 - SUPPORT (IN_VITRO)
    "T. thermosaccharolyticum GD17 significantly hampers the growth of C. thermocellum JN4"
  • PMID:31551972 - SUPPORT (IN_VITRO)
    "relieves the carbon catabolite repression of C. thermocellum JN4"

External Resources

Name Repository Resource ID
Exact-system mechanistic publication - JN4 and GD17 relationship
Primary open-access mechanistic study of the C. thermocellum JN4 and T. thermosaccharolyticum GD17 coculture. 		OTHER PMID:31551972
  • PMID:31551972 - SUPPORT (IN_VITRO)
    "Clostridium thermocellum JN4 and Thermoanaerobacterium thermosaccharolyticum GD17 coculture"
Exact-system hydrogenase publication - JN4 and GD17 hydrogen production mechanism
Follow-up exact-composition publication focused on why the JN4-GD17 coculture improves cellulosic hydrogen production. 		OTHER PMID:27538932
  • PMID:27538932 - SUPPORT (IN_VITRO)
    "co-culture of C. thermocellum JN4 and a non-cellulolytic bacterium Thermoanaerobacterium thermosaccharolyticum GD17"
Exact-system original hydrogen publication - cellulose-to-hydrogen coculture
Original report of hydrogen production from cellulose by the C. thermocellum JN4 and T. thermosaccharolyticum GD17 coculture. 		OTHER doi:10.1016/j.ijhydene.2008.04.004
  • PMID:27538932 - SUPPORT (IN_VITRO)
    "Previous investigations have shown that the co-culture of C. thermocellum JN4"

Environmental Factors

Factor Value Unit
Cellulose-rich lignocellulosic substrate cellulose N/A
  • PMID:31551972 - SUPPORT (IN_VITRO)
    "modulate cellulose degradation"
Released sugar intermediates glucose or cellobiose N/A
  • PMID:31551972 - SUPPORT (IN_VITRO)
    "conversion of glucose or cellobiose into final products"

Growth Media

Cellulose, cellobiose, or glucose anaerobic coculture assays

Atmosphere ANAEROBIC

Composition

Component Concentration Unit CHEBI
cellulose N/A N/A CHEBI:18246
cellobiose N/A N/A CHEBI:17057
glucose N/A N/A CHEBI:17234

Preparation notes: The record captures the substrate conditions supported by the abstract and does not assert complete salts, vitamins, or trace-element composition.

Evidence

PMID:31551972

"conversion of glucose or cellobiose into final products"

PMID:31551972

"modulate cellulose degradation"