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Coastal Forested Wetland Seawater-Ion Microcosm Community

Community ID CommunityMech:000258
Ecological State PERTURBED
Environment coastal forested freshwater wetland soil
ENVO:00000044

A restored coastal forested freshwater wetland soil community from the Timberlake Observatory for Wetland Restoration in North Carolina, curated around a manipulative laboratory microcosm experiment that disentangled sulfate from other seawater ions. Intact wetland soil cores received deionized water, artificial seawater, artificial seawater without sulfate, or sulfate alone, followed by DNA sequencing, biogeochemical measurements, and greenhouse gas flux measurements. The experiment found that artificial seawater altered microbial richness, composition, functional genes, and emissions of carbon dioxide, methane, and nitrous oxide whether sulfate was present or absent, whereas sulfate alone did not alter emissions or enrich sulfate-reducing bacteria or sulfur cycling genes. The record is DOE-relevant because authors were affiliated with the DOE Joint Genome Institute and Lawrence Berkeley National Laboratory, and sequencing followed DOE JGI methods.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
coastal forested wetland soil microbial community NCBITaxon:131567
PRIMARY_DEGRADER SECONDARY_FERMENTER SYNTROPHIC_PARTNER
N/A
  • PMID:38628812 - SUPPORT (IN_VITRO)
    "manipulative laboratory microcosm experiment"
  • PMID:38628812 - SUPPORT (COMPUTATIONAL)
    "combined with DNA sequencing and biogeochemical measurements"
coastal wetland sulfate-reducing bacteria NCBITaxon:2
PRIMARY_DEGRADER SECONDARY_FERMENTER
N/A
  • PMID:38628812 - SUPPORT (COMPUTATIONAL)
    "sulfate reducing bacteria or sulfur cycling genes"
  • PMID:38628812 - SUPPORT (IN_VITRO)
    "addition of sulfate alone did not lead to increases in the abundance of sulfate reducing bacteria"
coastal wetland carbon nitrogen phosphorus cycling guilds NCBITaxon:131567
PRIMARY_DEGRADER SECONDARY_FERMENTER CROSS_FEEDER
N/A
  • PMID:38628812 - SUPPORT (COMPUTATIONAL)
    "genes involved in carbon, nitrogen, and phosphorus cycling responded more strongly to artificial seawater than to sulfate"

Ecological Interactions

Ecological interaction network for Coastal Forested Wetland Seawater-Ion Microcosm Community Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Non-Sulfate Seawater Ions Drive Community And Emission Responses

NICHE_PARTITIONING

Metabolites: sulfate (CHEBI:16189), methane (CHEBI:16183), carbon dioxide (CHEBI:16526)

Biological Processes:

Evidence

  • PMID:38628812 - SUPPORT (IN_VITRO)
    "Greenhouse gas emissions and microbial richness and composition were altered by artificial seawater regardless of whether sulfate was present"
  • PMID:38628812 - SUPPORT (IN_VITRO)
    "other ions present in seawater, not sulfate, drive ecological and biogeochemical responses"

Sulfate Alone Does Not Enrich Sulfate-Reduction Features

COMPETITION

Source Taxon: coastal wetland sulfate-reducing bacteria

Metabolites: sulfate (CHEBI:16189)

Biological Processes:

Evidence

  • PMID:38628812 - SUPPORT (IN_VITRO)
    "sulfate alone did not alter emissions or communities"
  • PMID:38628812 - SUPPORT (IN_VITRO)
    "addition of sulfate alone did not lead to increases in the abundance of sulfate reducing bacteria or sulfur cycling genes"

Artificial Seawater Alters Carbon Nitrogen And Phosphorus Gene Response

NICHE_PARTITIONING

Source Taxon: coastal wetland carbon nitrogen phosphorus cycling guilds

Metabolites: methane (CHEBI:16183), carbon dioxide (CHEBI:16526)

Biological Processes:

  • organic substance catabolic process (GO:0071704)

Evidence

  • PMID:38628812 - SUPPORT (COMPUTATIONAL)
    "genes involved in carbon, nitrogen, and phosphorus cycling responded more strongly to artificial seawater than to sulfate"

Seawater-Ion Perturbation Of Greenhouse Gas Outputs

NICHE_PARTITIONING

Metabolites: methane (CHEBI:16183), carbon dioxide (CHEBI:16526)

Biological Processes:

Evidence

  • PMID:38628812 - SUPPORT (IN_VITRO)
    "emissions of carbon dioxide, methane, and nitrous oxide"
  • PMID:38628812 - SUPPORT (IN_VITRO)
    "may be drivers of increased methane emissions in soils that received artificial seawater addition"

External Resources

Name Repository Resource ID
Primary publication for coastal forested wetland seawater-ion microcosms
PubMed record for the ISME Communications article on sulfate and non-sulfate seawater ion effects on microbial communities and greenhouse gas emissions in a coastal forested wetland microcosm. 		OTHER PMID:38628812
  • PMID:38628812 - SUPPORT (OTHER)
    "Disentangling the effects of sulfate and other seawater ions"
DOI landing page
DOI for the ISME Communications publication. 		OTHER doi:10.1093/ismeco/ycae040
PubMed Central full text
Open-access full text with methods and discussion for the seawater-ion wetland microcosm study. 		OTHER PMCID:PMC11020224
  • PMID:38628812 - SUPPORT (OTHER)
    "Department of Energy Joint Genome Institute"

Environmental Factors

Factor Value Unit
Seawater intrusion simulation artificial seawater, artificial seawater without sulfate, sulfate only, and deionized-water control N/A
  • PMID:38628812 - SUPPORT (IN_VITRO)
    "tease apart the effects of sulfate from other seawater ions"
Coastal wetland salinization salt water components altering microbial community composition and function N/A
  • PMID:38628812 - SUPPORT (IN_VITRO)
    "consequences of coastal wetland salinization"
Greenhouse gases carbon dioxide, methane, and nitrous oxide N/A
  • PMID:38628812 - SUPPORT (IN_VITRO)
    "emissions of carbon dioxide, methane, and nitrous oxide"