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Corynebacterium glutamicum + Shewanella oneidensis Succinic-acid Co-culture

Community ID CommunityMech:000294
Ecological State ENGINEERED
Environment anaerobic bioreactor (succinic acid fermentation)
ENVO:00002123

Engineered two-member co-culture pairing the succinic-acid producer Corynebacterium glutamicum (including an ldhA-deleted, lactate-deficient strain K1) with the electroactive bacterium Shewanella oneidensis to enhance anaerobic succinic acid biosynthesis through interspecies interactions. Co-cultivation raised succinic acid yield markedly over monoculture without altering glucose consumption, and supplementation with riboflavin (the most effective of five screened redox-active additives) further improved production. Transcriptomics showed co-culture reprogrammed C. glutamicum metabolism, reinforcing its electron transport system and redistributing carbon flux toward the reductive TCA pathway.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Corynebacterium glutamicum (ldhA-deleted strain K1) NCBITaxon:1718
PRIMARY_PRODUCER
N/A
  • PMID:42203120 - SUPPORT (IN_VITRO)
    "an engineered co-culture system was developed by pairing C. glutamicum with the electroactive bacterium Shewanella oneidensis to enhance succinic acid biosynthesis through interspecies interactions"
Shewanella oneidensis NCBITaxon:70863
ELECTROGEN ELECTRON_DONOR
N/A
  • PMID:42203120 - SUPPORT (IN_VITRO)
    "an engineered co-culture system was developed by pairing C. glutamicum with the electroactive bacterium Shewanella oneidensis to enhance succinic acid biosynthesis through interspecies interactions"

Ecological Interactions

Ecological interaction network for Corynebacterium glutamicum + Shewanella oneidensis Succinic-acid Co-culture Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Riboflavin-mediated interspecies electron transfer enhancing succinic acid production

SYNTROPHY

Source Taxon: Shewanella oneidensis

Target Taxon: Corynebacterium glutamicum (ldhA-deleted strain K1)

Metabolites: riboflavin (CHEBI:17015)

Evidence

  • PMID:42203120 - SUPPORT (IN_VITRO)
    "Screening of five redox-active compounds identified riboflavin as the most effective additive, and supplementation with 0.1 mM riboflavin further improved production performance."
  • PMID:42203120 - SUPPORT (IN_VITRO)
    "Transcriptomic analysis revealed that co-culture induced global metabolic reprogramming in C. glutamicum, including reinforcement of the electron transport system, redistribution of carbon flux toward the reductive TCA pathway"

Growth Media

unspecified (glucose-based anaerobic succinic acid fermentation medium)

Atmosphere ANAEROBIC

Preparation notes: Anaerobic succinic acid fermentation with glucose as carbon source; the optimized co-culture was supplemented with 0.1 mM riboflavin as a redox-active additive. Only the reference abstract was accessible (full text subscription-required); medium name, temperature, pH, and vessel details were not reported in the accessible source.

Evidence

PMID:42203120

"Anaerobic production of succinic acid in Corynebacterium glutamicum is often constrained by metabolic imbalances and by-product formation."

PMID:42203120

"supplementation with 0.1 mM riboflavin further improved production performance"