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Crucian Carp Gut Disease-resistance SynCom

Community ID CommunityMech:000293
Ecological State ENGINEERED
Environment crucian carp (Carassius auratus) intestine
ENVO:2100002

A simplified synthetic microbial community of three gut genera (Cetobacterium, Paraclostridium, and Pseudomonas) assembled from the intestinal microbiota of crucian carp (Carassius auratus). These genera were enriched in fish that displayed mild symptoms after Aeromonas hydrophila infection, and fecal microbiota transplantation from mild-symptom fish conferred enhanced resistance to the pathogen. The reconstituted SynCom significantly reduced A. hydrophila abundance by activating intestinal immune responses and reinforcing the gut barrier, supporting microbiome-based disease prevention in aquaculture.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Cetobacterium NCBITaxon:180162 N/A
  • PMID:41280275 - SUPPORT (IN_VIVO)
    "Further microbial analysis identified Cetobacterium (p = 0.013), Paraclostridium"
Paraclostridium NCBITaxon:1849822 N/A
  • PMID:41280275 - SUPPORT (IN_VIVO)
    "Further microbial analysis identified Cetobacterium (p = 0.013), Paraclostridium"
Pseudomonas NCBITaxon:286 N/A
  • PMID:41280275 - SUPPORT (IN_VIVO)
    "Paraclostridium (p Pseudomonas (p A. hydrophila (p < 0.05) by activating intestinal immune responses and reinforcing the gut barrier."

Ecological Interactions

Ecological interaction network for Crucian Carp Gut Disease-resistance SynCom Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

SynCom-mediated suppression of Aeromonas hydrophila

COMPETITION

Target Taxon: Aeromonas hydrophila

Evidence

  • PMID:41280275 - SUPPORT (IN_VIVO)
    "Paraclostridium (p Pseudomonas (p A. hydrophila (p < 0.05) by activating intestinal immune responses and reinforcing the gut barrier."

Growth Media

BHI and LB (brain heart infusion and Luria-Bertani)

Temperature 28 °C

Preparation notes: SynCom strains isolated on BHI and LB agar (48 h, anaerobic at 28 C and aerobic), then expanded in 5 mL BHI/LB broth with overnight shaking to OD600 1.0 before combining.

Evidence

PMID:41280275

"Plates were incubated for 48 h under both anaerobic conditions at 28 °C and aerobic conditions"

PMID:41280275

"inoculated into 5 mL of BHI and LB broth for expansion"