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Jeff Dangl's SynComm 35

Ecological State ENGINEERED
Environment rhizosphere
ENVO:00005801
Source File Dangl_SynComm_35.yaml

A 35-member synthetic community (SynComm 35) composed of genome-sequenced bacterial strains representing typical taxonomic diversity of Arabidopsis root commensals. This community was designed to interrogate plant immune responses, particularly pattern-triggered immunity (PTI/MTI). Of the 35 members, 10 are robust suppressors of MAMP-triggered immunity (flg22 response), 13 are partial suppressors, and 11 are non-suppressors. The community includes 32 strains isolated from Brassicaceae roots (mainly Arabidopsis), 2 strains from unplanted soil, and E. coli DH5α as a control. SynComm 35 collectively suppresses specific transcriptional sectors of plant immunity, particularly WRKY transcription factors, receptor-like kinases, and secondary metabolite biosynthesis genes, while suppressors achieve enhanced colonization capacity.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Arabidopsis thaliana NCBITaxon:3702
PRIMARY_PRODUCER
N/A
Dyella japonica MF79 NCBITaxon:231455
SYNTROPHIC_PARTNER
COMMON
Xanthomonadales members NCBITaxon:135614
SYNTROPHIC_PARTNER
COMMON
Pseudomonas species NCBITaxon:286
SYNTROPHIC_PARTNER
COMMON
Flavobacterium species NCBITaxon:237
SYNTROPHIC_PARTNER
COMMON
Stenotrophomonas species NCBITaxon:40323
SYNTROPHIC_PARTNER
COMMON
Microbacterium species NCBITaxon:33882
SYNTROPHIC_PARTNER
COMMON
Escherichia coli DH5α NCBITaxon:668369
CROSS_FEEDER
COMMON

Ecological Interactions

MAMP-Triggered Immunity Suppression

COMMENSALISM

Source Taxon: SynComm 35 bacterial community

Biological Processes:

  • pattern recognition receptor signaling pathway (GO:0002221)
  • defense response to bacterium (GO:0042742)
  • regulation of innate immune response (GO:0045088)

Evidence

  • doi:10.1073/pnas.2100678118 - SUPPORT (IN_VITRO)
    "Significance In natural environments, plants establish intimate interactions with a wide diversity of microbes"

WRKY Transcription Factor Suppression

COMMENSALISM

Source Taxon: Bacterial suppressors (10 robust strains)

Biological Processes:

  • regulation of defense response (GO:0031347)
  • regulation of transcription, DNA-templated (GO:0006355)

Evidence

  • doi:10.1073/pnas.2100678118 - SUPPORT (IN_VITRO)
    "It is unknown, however, how microbiota composed of commensal bacteria colonize roots in the face of a sophisticated plant immune system that evolved to recognize microbial-associated molecular patterns"

Type II Secretion-Mediated Immune Modulation

COMMENSALISM

Source Taxon: Dyella japonica MF79

Biological Processes:

Evidence

  • doi:10.1073/pnas.2100678118 - SUPPORT (IN_VITRO)
    "We highlight the role of a specific sector of the plant immune system and its suppression in gating microbial access to the roots."

Enhanced Colonization Through Community Cooperation

MUTUALISM

Source Taxon: Suppressor bacteria

Biological Processes:

  • interspecies interaction between organisms (GO:0044419)

Evidence

  • doi:10.1073/pnas.2100678118 - SUPPORT (IN_VITRO)
    "Suppressors and nonsuppressors co-occur in the root microbiome and the presence of the former can enhance the colonization ability of the latter"

Environmental Factors

Factor Value Unit
MAMP Treatment flg22 (flagellin epitope) nM or µM
Plant Age 7 days N/A
Colonization Period 12-14 days N/A
Growth Conditions axenic N/A
Immune Response Type Pattern-triggered immunity (PTI/MTI) N/A
Suppressor Classification 10 robust, 13 partial, 11 non-suppressors N/A