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Dehalococcoides-Pelobacter Acetylene TCE Coculture

Community ID CommunityMech:000184
Ecological State ENGINEERED
Environment anaerobic chlorinated-solvent laboratory coculture
ENVO:01001405

A defined anaerobic laboratory coculture in which the acetylene-fermenting bacterium historically reported as Pelobacter strain SFB93 is paired with Dehalococcoides mccartyi strain 195 to sustain reductive dechlorination of trichloroethene when acetylene is provided as electron donor and carbon source. The system is relevant to chlorinated-solvent groundwater bioremediation because acetylene can be generated during abiotic TCE degradation and can inhibit dechlorination at high concentrations.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Dehalococcoides mccartyi strain 195 NCBITaxon:243164
PRIMARY_DEGRADER SYNTROPHIC_PARTNER
N/A
  • PMID:28075122 - SUPPORT (IN_VITRO)
    "D. mccartyi strain 195"
  • PMID:28075122 - SUPPORT (IN_VITRO)
    "reductive dechlorination of TCE by Dehalococcoides mccartyi"
Pelobacter strain SFB93 NCBITaxon:18
SECONDARY_FERMENTER SYNTROPHIC_PARTNER
N/A
  • PMID:28075122 - SUPPORT (IN_VITRO)
    "Pelobacter SFB93, a C2H2-fermenting bacterium"
  • PMID:28075122 - SUPPORT (IN_VITRO)
    "adding Pelobacter SFB93 into the consortium"

Ecological Interactions

Ecological interaction network for Dehalococcoides-Pelobacter Acetylene TCE Coculture Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Acetylene-Fueled Dechlorinating Coculture

SYNTROPHY

Source Taxon: Pelobacter strain SFB93

Target Taxon: Dehalococcoides mccartyi strain 195

Metabolites: acetylene (CHEBI:27518), trichloroethene (CHEBI:16602)

Biological Processes:

  • interspecies interaction between organisms (GO:0044419)

Evidence

  • PMID:28075122 - SUPPORT (IN_VITRO)
    "acetylene as the electron donor and carbon source"
  • PMID:28075122 - SUPPORT (IN_VITRO)
    "TCE or cis-DCE served as the electron acceptor"

D. mccartyi Reductive Dechlorination

CROSS_FEEDING

Source Taxon: Dehalococcoides mccartyi strain 195

Target Taxon: Pelobacter strain SFB93

Metabolites: trichloroethene (CHEBI:16602), chloroethene (CHEBI:28509)

Biological Processes:

Evidence

  • PMID:28075122 - SUPPORT (IN_VITRO)
    "reductive dechlorination of TCE by Dehalococcoides mccartyi"
  • PMID:28075122 - SUPPORT (IN_VITRO)
    "genes encoding for reductive dehalogenases"

SFB93-Mediated Relief of Acetylene Inhibition

COLONIZATION_FACILITATION

Source Taxon: Pelobacter strain SFB93

Metabolites: acetylene (CHEBI:27518)

Biological Processes:

  • interspecies interaction between organisms (GO:0044419)

Evidence

  • PMID:28075122 - SUPPORT (IN_VITRO)
    "inhibition was removed by adding Pelobacter SFB93"

Associated Datasets

Dataset Type Repository Accession
Dehalococcoides mccartyi 195 genome BioProject
Genome project for D. mccartyi 195, the TCE-dechlorinating partner in the coculture. 		GENOME NCBI_BIOPROJECT PRJNA214
Pelobacter strain SFB93 genome BioProject
Genome project containing the SFB93 acetylene-fermenting partner. 		GENOME NCBI_BIOPROJECT PRJNA319824

External Resources

Name Repository Resource ID
Exact-system primary publication - D. mccartyi 195 and Pelobacter SFB93
Primary publication describing acetylene-fed defined Dehalococcoides/Pelobacter consortia for TCE reductive dechlorination, including D. mccartyi strain 195 with SFB93. 		OTHER PMID:28075122
  • PMID:28075122 - SUPPORT (IN_VITRO)
    "with D. mccartyi strain 195"
SFB93 genome publication
Genome announcement for the acetylene-fermenting Pelobacter sp. strain SFB93. 		OTHER doi:10.1128/genomeA.01573-16

Environmental Factors

Factor Value Unit
Acetylene co-contaminant acetylene generated during TCE degradation N/A
  • PMID:28075122 - SUPPORT (IN_VITRO)
    "generated in contaminated groundwater sites"
High acetylene inhibition 1.3 mM
  • PMID:28075122 - SUPPORT (IN_VITRO)
    "high C2H2 (1.3 mM) concentrations reversibly inhibit"
Low acetylene compatibility 0.4 mM
  • PMID:28075122 - SUPPORT (IN_VITRO)
    "low C2H2 (0.4 mM) concentrations do not inhibit"

Growth Media

Acetylene and chlorinated-ethene anaerobic coculture assay

Atmosphere ANAEROBIC

Composition

Component Concentration Unit CHEBI
acetylene N/A N/A CHEBI:27518
trichloroethene N/A N/A CHEBI:16602
cis-dichloroethene N/A N/A N/A

Preparation notes: The abstract supports acetylene-fed anaerobic cocultures with TCE or cis-DCE as electron acceptor; detailed medium salts are not asserted here.

Evidence

PMID:28075122

"acetylene as the electron donor and carbon source"

PMID:28075122

"TCE or cis-DCE served as the electron acceptor"