A defined anaerobic laboratory coculture in which the acetylene-fermenting bacterium historically reported as Pelobacter strain SFB93 is paired with Dehalococcoides mccartyi strain 195 to sustain reductive dechlorination of trichloroethene when acetylene is provided as electron donor and carbon source. The system is relevant to chlorinated-solvent groundwater bioremediation because acetylene can be generated during abiotic TCE degradation and can inhibit dechlorination at high concentrations.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Dehalococcoides mccartyi strain 195 — ontology label: Dehalococcoides mccartyi 195 | NCBITaxon:243164 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
SYNTROPHIC_PARTNER
|
N/A |
|
|||
| Pelobacter strain SFB93 — ontology label: Pelobacter | NCBITaxon:18 · TaxonMech · TraitMech |
SECONDARY_FERMENTER
SYNTROPHIC_PARTNER
|
N/A |
|
|||
Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Acetylene-Fueled Dechlorinating Coculture
SYNTROPHYSFB93 fermentation allows acetylene to supply electron donor and carbon for the defined strain-195 coculture through hydrogen and acetate provision. Strain 195 reductively dechlorinates TCE, predominantly to vinyl chloride under the reported conditions. The BAV1 coculture and the ANAS enrichment are separate systems.
Source Taxon: Pelobacter strain SFB93
Target Taxon: Dehalococcoides mccartyi strain 195
Metabolites: acetylene (CHEBI:27518 · MediaIngredientMech · CMMMech), trichloroethene (CHEBI:16602 · MediaIngredientMech · CMMMech)
Biological Processes:
- interspecies interaction between organisms (GO:0044419 · PathwayMech)
SFB93 acetylene fermentation supplies hydrogen and acetate that support strain-195 TCE dechlorination and growth. This positive nutritional coupling is distinct from removal of an initially inhibitory acetylene exposure; it does not assign BAV1 vinyl-chloride respiration to strain 195.
Evidence
-
PMID:28075122 - SUPPORT (IN_VITRO)"acetylene as the electron donor and carbon source"
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PMID:28075122 - SUPPORT (IN_VITRO)"TCE or cis-DCE served as the electron acceptor"
D. mccartyi Reductive Dechlorination
Strain 195 performs hydrogen-dependent reductive dechlorination of TCE in the coculture, predominantly producing vinyl chloride. This is the recipient phenotype, not nutrient transfer back to SFB93. In separate acetylene-inhibited strain-195 assays, unchanged tceA transcript abundance does not establish induction-mediated rescue or identify the inhibited enzyme.
Source Taxon: Dehalococcoides mccartyi strain 195
Metabolites: trichloroethene (CHEBI:16602 · MediaIngredientMech · CMMMech), chloroethene (CHEBI:28509 · MediaIngredientMech · CMMMech)
Biological Processes:
- anaerobic respiration (GO:0009061 · PathwayMech)
Evidence
-
PMID:28075122 - SUPPORT (IN_VITRO)"reductive dechlorination of TCE by Dehalococcoides mccartyi"
-
PMID:28075122 - PARTIAL (IN_VITRO)"genes encoding for reductive dehalogenases"
SFB93-Mediated Relief of Acetylene Inhibition
Adding SFB93 to acetylene-inhibited strain-195 cultures consumed acetylene and restored TCE reductive dechlorination in the primary study. This exact-pair rescue is distinct from the separate ANAS enrichment and its condition-dependent methanogenesis response; it is detoxification support, not demonstrated colonization facilitation.
Source Taxon: Pelobacter strain SFB93
Target Taxon: Dehalococcoides mccartyi strain 195
Metabolites: acetylene (CHEBI:27518 · MediaIngredientMech · CMMMech)
Biological Processes:
- interspecies interaction between organisms (GO:0044419 · PathwayMech)
SFB93 consumption of inhibitory acetylene permits strain 195 to resume TCE reductive dechlorination, as tested by delayed partner addition in the primary Results and Figure 2 caption. The local abstract rescue phrase concerns ANAS and alone does not establish this exact-pair result; ANAS methanogenesis is not transferred to this arrow.
Evidence
-
PMID:28075122 - PARTIAL (IN_VITRO)"inhibition was removed by adding Pelobacter SFB93"
Associated Datasets
| Dataset | Type | Repository | Accession |
|---|---|---|---|
|
Dehalococcoides mccartyi 195 genome BioProject
Genome project for D. mccartyi 195, the TCE-dechlorinating partner in the coculture. |
GENOMICS | NCBI_BIOPROJECT | PRJNA214 |
|
Pelobacter strain SFB93 genome BioProject
Genome project containing the SFB93 acetylene-fermenting partner. |
GENOMICS | NCBI_BIOPROJECT | PRJNA319824 |
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which acetylene-sensitive processes limit strain-195 recovery after SFB93 addition?
The whole primary main body and six figure captions were read. Figure 2 reports exact strain-195 rescue after delayed SFB93 addition; the cached abstract rescue sentence instead concerns ANAS. Unchanged tceA transcription does not identify the inhibited enzyme. Distinguish the nutritional coculture, inhibited-isolate rescue and ANAS methanogenesis assays. Exact-pair body evidence is not locally snippet-verifiable. Existing non-graph evidence debt remains under #765; the curated Syntrophotalea grounding is unchanged. No figure images, Table 1 or supplements were inspected.
Attaches to: ecological_interactions#Acetylene-Fueled Dechlorinating Coculture, ecological_interactions#D. mccartyi Reductive Dechlorination, ecological_interactions#SFB93-Mediated Relief of Acetylene Inhibition
External Resources
| Name | Repository | Resource ID |
|---|---|---|
|
Exact-system primary publication - D. mccartyi 195 and Pelobacter SFB93
Primary publication describing acetylene-fed defined Dehalococcoides/Pelobacter consortia for TCE reductive dechlorination, including D. mccartyi strain 195 with SFB93. |
OTHER | PMID:28075122 |
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|
SFB93 genome publication
Genome announcement for the acetylene-fermenting Pelobacter sp. strain SFB93. |
OTHER | doi:10.1128/genomeA.01573-16 |
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Acetylene co-contaminant | acetylene generated during TCE degradation | N/A |
|
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| High acetylene inhibition | 1.3 | mM |
|
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| Low acetylene compatibility | 0.4 | mM |
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Growth Media
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