A five-member desert-root bacterial SynCom that protects tomato plants from high salt stress in non-sterile soil.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
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| Massilia sp. SA087 — ontology label: Massilia | NCBITaxon:149698 · TaxonMech · TraitMech | N/A | |
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| Enterobacter sp. SA187 — ontology label: Enterobacter | NCBITaxon:547 · TaxonMech · TraitMech | N/A | |
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| Ensifer sp. SA403 — ontology label: Ensifer | NCBITaxon:106591 · TaxonMech · TraitMech | N/A | |
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| Bacillus sp. SA436 — ontology label: Bacillus <firmicutes> | NCBITaxon:1386 · TaxonMech · TraitMech | N/A | |
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| Streptomyces sp. SA444 — ontology label: Streptomyces | NCBITaxon:1883 · TaxonMech · TraitMech | N/A | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
SynCom C promotion of salt-stressed tomato biomass
SynCom C increased salt-stressed tomato biomass. The comparison in which none of its five individual strains significantly promoted growth used sterile river sand; this does not negate single-strain benefits in the separate nonsterile screen.
Participating Taxa: Massilia sp. SA087, Enterobacter sp. SA187, Ensifer sp. SA403, Bacillus sp. SA436, Streptomyces sp. SA444
Biological Processes:
- response to salt stress (GO:0009651 · PathwayMech)
Evidence
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PMID:35444261 - SUPPORT (IN_VIVO)"SynCom C was the only inoculum that significantly promoted tomato growth compared to the inoculum-free control"
SynCom C activation of root SOS marker expression
SynCom C triggered an early tomato root salt-stress response, including significant upregulation of SOS1, SOS2, and WRKY8 four days after salt imposition.
Participating Taxa: Massilia sp. SA087, Enterobacter sp. SA187, Ensifer sp. SA403, Bacillus sp. SA436, Streptomyces sp. SA444
Biological Processes:
- response to salt stress (GO:0009651 · PathwayMech)
PARTIAL - early SOS-associated expression could support ion transport and thereby reduce later shoot ionic imbalance. Expression was measured, but its mediation of the ion phenotype was not selectively perturbed in this SynCom experiment.
Evidence
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PMID:35444261 - SUPPORT (IN_VIVO)"SOS1, SOS2 and WRKY8 expression was significantly upregulated in the root of tomato plants treated with SynCom C"
SynCom C reduction of shoot sodium-potassium imbalance
SynCom C-treated salt-stressed tomato plants accumulated a lower shoot Na+/K+ ratio than uninoculated controls, consistent with lower ion toxicity under salt stress.
Participating Taxa: Massilia sp. SA087, Enterobacter sp. SA187, Ensifer sp. SA403, Bacillus sp. SA436, Streptomyces sp. SA444
Metabolites: sodium(1+) (CHEBI:29101 · MediaIngredientMech · CMMMech), potassium(1+) (CHEBI:29103 · MediaIngredientMech · CMMMech)
Biological Processes:
- sodium ion transport (GO:0006814 · PathwayMech)
PARTIAL - improved shoot ion balance could reduce salt injury and support biomass accumulation. Treatment-associated endpoints support this interpretation without selectively establishing ion balance as the mediator.
Evidence
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PMID:35444261 - SUPPORT (IN_VIVO)"Na+/K+ ratio was significantly lower in SynCom C inoculated plants"
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PMID:35444261 - SUPPORT (IN_VIVO)"Here we show that a SynCom of five bacterial strains, originating from the root of the desert plant Indigofera argentea, protected tomato plants growing in a non-sterile substrate against a high salt stress."
SynCom C five-strain salt protection
SynCom C was the strongest reduced community from a 20-combination screen of non-redundant three- to five-strain subsets of the 15-strain Jizan library in salt-stressed tomato.
Participating Taxa: Massilia sp. SA087, Enterobacter sp. SA187, Ensifer sp. SA403, Bacillus sp. SA436, Streptomyces sp. SA444
Biological Processes:
- response to salt stress (GO:0009651 · PathwayMech)
Evidence
-
PMID:35444261 - SUPPORT (IN_VIVO)"the five member SynCom C performed the best and was the only one significantly different to the control"
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which host processes and consortium members mediate SynCom C salt protection?
Whole-host treatment responses do not establish reciprocal microbial benefit or member necessity. Preserve the sterile/nonsterile assay distinction and the earlier screen-to-validation workflow-edge removal. The larger Jizan tracking experiment is not a five-member functional perturbation. Non-graph evidence classification remains open under #1397.
Attaches to: ecological_interactions#SynCom C promotion of salt-stressed tomato biomass, ecological_interactions#SynCom C activation of root SOS marker expression, ecological_interactions#SynCom C reduction of shoot sodium-potassium imbalance, ecological_interactions#SynCom C five-strain salt protection
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| defined synthetic community design | Five bacterial strains originating from the root of Indigofera argentea were assembled into a SynCom. | N/A |
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Growth Media
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