A natural, depth-resolved microbial community from a hillslope-to-riparian transect in the DOE-supported East River watershed near Crested Butte, Colorado. Metagenomic and geochemical analyses showed that distance from the East River, proximity to the groundwater table, and underlying weathered shale strongly structure microbial community composition and metabolic potential. Riparian-zone communities are compositionally distinct from hillslope communities and are functionally differentiated by capacities for carbon fixation, nitrogen fixation, sulfate reduction, Candidate Phyla Radiation bacteria in saturated sediments, and selenium reduction at depth.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| East River hillslope-riparian transect microbiome | NCBITaxon:131567 |
PRIMARY_DEGRADER
PRIMARY_PRODUCER
|
N/A |
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| riparian zone microbial communities | NCBITaxon:131567 |
PRIMARY_DEGRADER
PRIMARY_PRODUCER
|
DOMINANT |
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| riparian saturated sediment Candidate Phyla Radiation bacteria | NCBITaxon:1783234 |
CROSS_FEEDER
SYNTROPHIC_PARTNER
|
N/A |
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| hillslope microbial communities | NCBITaxon:131567 |
PRIMARY_DEGRADER
|
N/A |
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Ecological Interactions
Groundwater and Weathered Shale Niche Filtering
NICHE_PARTITIONINGEvidence
-
PMID:31380022 - SUPPORT (COMPUTATIONAL)"proximity to groundwater and underlying weathered shale"
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PMID:31380022 - SUPPORT (COMPUTATIONAL)"strongly impact microbial community structure and metabolic potential"
Riparian Carbon Nitrogen and Sulfur Functional Zone
NICHE_PARTITIONINGMetabolites: carbon dioxide (CHEBI:16526), sulfate (CHEBI:16189)
Biological Processes:
- carbon fixation (GO:0015977)
- nitrogen fixation (GO:0009399)
- sulfate reduction (GO:0019419)
Evidence
-
PMID:31380022 - SUPPORT (COMPUTATIONAL)"capacities for carbon and nitrogen fixation and sulfate reduction"
-
PMID:31380022 - SUPPORT (COMPUTATIONAL)"functionally differentiated from hillslope communities"
Depth-Enriched Uncultivated Bacterial Lineages
NICHE_PARTITIONINGSource Taxon: riparian saturated sediment Candidate Phyla Radiation bacteria
Evidence
-
PMID:31380022 - SUPPORT (COMPUTATIONAL)"consistently increase in abundance with increasing depth"
-
PMID:31380022 - SUPPORT (COMPUTATIONAL)"riparian zone saturated sediments we found Candidate Phyla Radiation bacteria"
Weathered Shale and Saturated Sediment Selenium Reduction
NICHE_PARTITIONINGBiological Processes:
- oxidation-reduction process (GO:0055114)
Evidence
-
PMID:31380022 - SUPPORT (COMPUTATIONAL)"Selenium reduction is prominent at depth"
-
PMID:31380022 - SUPPORT (COMPUTATIONAL)"weathered shale and saturated riparian zone sediments"
Associated Datasets
| Dataset | Type | Repository | Accession |
|---|---|---|---|
|
East River hillslope metagenome BioProject example
NCBI BioProject record for a DOE JGI East River watershed hillslope metagenome sample associated with groundwater sediment microbial communities from the East River, Colorado. |
METAGENOME | NCBI_BIOPROJECT | PRJNA444173 |
External Resources
| Name | Repository | Resource ID |
|---|---|---|
|
Primary publication for the East River hillslope-riparian transect community
PubMed record for the East River hillslope-to-riparian transect metagenomic and geochemical community study. |
OTHER | PMID:31380022 |
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DOI landing page
DOI landing page for the Ecology and Evolution article. |
OTHER | doi:10.1002/ece3.5254 |
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DOE PAGES record
DOE PAGES record for the East River hillslope-riparian transect article. |
OTHER | OSTI:1560176 |
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Hillslope-to-riparian transect position | depth-resolved hillslope to riparian zone transect | N/A |
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| Groundwater table proximity | proximity to groundwater table | N/A |
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| Weathered shale | underlying weathered shale | N/A |
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