KB-1 is a natural anaerobic bioaugmentation consortium used for reductive dechlorination of chlorinated ethenes in contaminated groundwater. The culture contains Dehalococcoides-related organohalide-respiring bacteria that convert tetrachloroethene and trichloroethene through chlorinated intermediates to ethene. Nondechlorinating acetogens, including Acetobacterium and Sporomusa, support the community by providing cobamide-related cofactors needed by Dehalococcoides.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Dehalococcoides spp. in KB-1 | NCBITaxon:61434 |
PRIMARY_DEGRADER
|
N/A |
|
|||
| Acetobacterium spp. in KB-1 | NCBITaxon:33952 |
SYNTROPHIC_PARTNER
|
N/A |
|
|||
| Sporomusa spp. in KB-1 | NCBITaxon:906 |
SYNTROPHIC_PARTNER
|
N/A |
|
|||
Ecological Interactions
Reductive Dechlorination to Ethene
SYNTROPHYSource Taxon: Dehalococcoides spp. in KB-1
Metabolites: trichloroethene (CHEBI:16602), chloroethene (CHEBI:28509), ethene (CHEBI:18153)
Evidence
-
PMID:12523427 - SUPPORT (IN_VIVO)"PCE, TCE, and cis-1,2-DCE within the PTA were all below"
-
PMID:12523427 - SUPPORT (IN_VIVO)"ethene production accounted for the observed mass loss"
Acetogen Cobamide Support
CROSS_FEEDINGSource Taxon: Acetobacterium spp. in KB-1
Target Taxon: Dehalococcoides spp. in KB-1
Metabolites: cobalamin (CHEBI:30411)
Biological Processes:
- vitamin metabolic process (GO:0006766)
Evidence
-
PMID:31441646 - SUPPORT (IN_VITRO)"the lower ligand of cobalamin, and Sporomusa are dominant acetogens"
-
PMID:31441646 - SUPPORT (IN_VITRO)"substantial increase in cobalamin"
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Anaerobic contaminated groundwater | Reducing conditions after methanol and acetate amendment | N/A |
|
||
| pH limit for vinyl chloride dechlorination | Sustained VC-to-ethene dechlorination at pH 5.5; inhibition below pH 5.0 | N/A |
|
||