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Lotus Lj-SC3 Synthetic Community

Ecological State ENGINEERED
Environment rhizosphere
ENVO:00005801
Source File Lotus_LjSC3.yaml

A 16-member synthetic community (Lj-SC3) derived from Lotus japonicus roots and nodules, designed to study host preference and community assembly dynamics in legume-microbe interactions. The community represents bacterial families from the Lj-SPHERE culture collection, including nitrogen-fixing symbionts from Rhizobiaceae and Phyllobacteriaceae families, as well as commensal bacteria from Burkholderiaceae, Oxalobacteriaceae, Pseudomonadaceae, and Microbacteriaceae. This community exhibits host preference when co-inoculated with Arabidopsis-derived strains, preferentially colonizing Lotus roots in a community context. The SC3 community enables investigation of nitrogen fixation, nodulation, priority effects during microbiota assembly, and bacterial invasiveness in the rhizosphere.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Mesorhizobium sp. LjNodule210 NCBITaxon:68287
PRIMARY_PRODUCER
DOMINANT
Mesorhizobium sp. LjNodule215 NCBITaxon:68287
PRIMARY_PRODUCER
COMMON
Mesorhizobium sp. LjNodule218 NCBITaxon:68287
PRIMARY_PRODUCER
COMMON
Phyllobacteriaceae bacterium NCBITaxon:1871068
SYNTROPHIC_PARTNER
ABUNDANT
Rhizobiaceae bacterium NCBITaxon:1913961
SYNTROPHIC_PARTNER
ABUNDANT
Burkholderiaceae bacterium NCBITaxon:2030806
SYNTROPHIC_PARTNER
COMMON
Oxalobacteriaceae bacterium NCBITaxon:75682
SYNTROPHIC_PARTNER
COMMON
Pseudomonadaceae bacterium NCBITaxon:1914538
SYNTROPHIC_PARTNER
COMMON
Microbacteriaceae bacterium NCBITaxon:1873462
SYNTROPHIC_PARTNER
COMMON
Lotus japonicus NCBITaxon:34305
PRIMARY_PRODUCER
N/A

Ecological Interactions

Symbiotic Nitrogen Fixation

MUTUALISM

Source Taxon: Mesorhizobium sp.

Metabolites: dinitrogen (CHEBI:17997), ammonia (CHEBI:16134)

Biological Processes:

Evidence

  • PMID:34312531 - SUPPORT (IN_VITRO)
    "Sequential inoculation experiments revealed priority effects during root microbiota assembly, where established communities are resilient to invasion by latecomers, and that host preference of commensal bacteria confers a competitive advantage in their cognate host"

Host Preference and Selective Colonization

MUTUALISM

Source Taxon: Phyllobacteriaceae bacterium

Biological Processes:

Evidence

  • PMID:34312531 - SUPPORT (IN_VITRO)
    "Sequential inoculation experiments revealed priority effects during root microbiota assembly, where established communities are resilient to invasion by latecomers, and that host preference of commensal bacteria confers a competitive advantage in their cognate host"

Priority Effects and Community Assembly

COMPETITION

Biological Processes:

  • interspecies interaction between organisms (GO:0044419)

Evidence

  • PMID:34312531 - SUPPORT (IN_VITRO)
    "Sequential inoculation experiments revealed priority effects during root microbiota assembly, where established communities are resilient to invasion by latecomers, and that host preference of commensal bacteria confers a competitive advantage in their cognate host"

Plant Growth Promotion

MUTUALISM

Biological Processes:

Evidence

  • PMID:34312531 - SUPPORT (IN_VITRO)
    "It remains unclear to what extent the host actively selects these communities and whether commensals are adapted to a specific plant species"

Environmental Factors

Factor Value Unit
Host plant species Lotus japonicus N/A
Nodulation status nitrogen-fixing nodules present N/A
Community context dependence host preference requires multi-species community N/A
Priority effects invasion resistance N/A
Taxonomic diversity 16 bacterial families N/A
Agricultural relevance legume-microbe symbiosis model N/A