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MAMC-M48 Lignocellulose-Degrading Consortium

Community ID CommunityMech:000041
Ecological State ENGINEERED
Environment soil
ENVO:00001998 · HabitatMech ↗

A minimal active microbial consortium (MAMC-M48) for lignocellulose degradation, optimized through reductive screening from 18 candidate strains. The 5-member consortium consists of Stenotrophomonas maltophilia JN40, Paenibacillus sp. PALXIL05, Microbacterium sp. UYFA68, Chryseobacterium taiwanense DUCC3723, and Brevundimonas sp. R3. Enriched from soil bacteria on sugarcane waste, this synthetic community achieves 50.6% degradation of all lignocellulose fractions, reaching 96.5% of the degradation rate observed with the full 18-species pool, demonstrating high efficiency with minimal complexity.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Stenotrophomonas maltophilia JN40 — ontology label: Stenotrophomonas maltophilia NCBITaxon:40324 · TaxonMech · TraitMech
PRIMARY_DEGRADER
DOMINANT
  • PMID:29392382 - SUPPORT (IN_VITRO)
    "A consortium containing Stenotrophomonas maltophilia, Paenibacillus sp., Microbacterium sp., Chryseobacterium taiwanense, and Brevundimonas sp. was found to be the most effective degrading synthetic community."
Paenibacillus sp. PALXIL05 NCBITaxon:375486 · TaxonMech · TraitMech
PRIMARY_DEGRADER
DOMINANT
  • PMID:29392382 - SUPPORT (IN_VITRO)
    "A consortium containing Stenotrophomonas maltophilia, Paenibacillus sp., Microbacterium sp., Chryseobacterium taiwanense, and Brevundimonas sp. was found to be the most effective degrading synthetic community."
Microbacterium sp. UYFA68 NCBITaxon:1605331 · TaxonMech · TraitMech
PRIMARY_DEGRADER
DOMINANT
  • PMID:29392382 - SUPPORT (IN_VITRO)
    "A consortium containing Stenotrophomonas maltophilia, Paenibacillus sp., Microbacterium sp., Chryseobacterium taiwanense, and Brevundimonas sp. was found to be the most effective degrading synthetic community."
Chryseobacterium taiwanense DUCC3723 — ontology label: Chryseobacterium taiwanense NCBITaxon:363331 · TaxonMech · TraitMech
PRIMARY_DEGRADER
DOMINANT
  • PMID:29392382 - SUPPORT (IN_VITRO)
    "A consortium containing Stenotrophomonas maltophilia, Paenibacillus sp., Microbacterium sp., Chryseobacterium taiwanense, and Brevundimonas sp. was found to be the most effective degrading synthetic community."
Brevundimonas sp. R3 NCBITaxon:1501972 · TaxonMech · TraitMech
PRIMARY_DEGRADER
COMMON
  • PMID:29392382 - SUPPORT (IN_VITRO)
    "A consortium containing Stenotrophomonas maltophilia, Paenibacillus sp., Microbacterium sp., Chryseobacterium taiwanense, and Brevundimonas sp. was found to be the most effective degrading synthetic community."

Ecological Interactions

The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.

Ecological interaction network for MAMC-M48 Lignocellulose-Degrading Consortium Bipartite graph where circles represent taxa and non-circular symbols represent interactions. Rare or untyped interactions share the Other symbol; exact curated types remain in the interaction details.
Taxon
Other

MAMC-M48 Lignocellulose Degradation

MAMC-M48, containing study isolate IDs 13, 18, 25, 29 and 68, was the best-performing five-member mixture in the tested 65-mixture screen. At 96 hours, calibrated FTIR estimates were 43.01% lignin, 39.65% cellulose and 69.18% hemicellulose change, averaging 50.6%, rather than 50.6% for each fraction. These are fraction-composition estimates, not direct gravimetric mass loss, carbon mineralization or partner-resolved catalytic flux. HYPOTHESIZED - complementary substrate use could contribute, but niche partitioning and directed exchanges were not established. Canonical participants retain source-reported 16S identification labels; their strain-like suffixes are not independently verified culture-strain identities. Enrichment and reductive screening describe selection procedures, not ecological interactions.

Participating Taxa: Stenotrophomonas maltophilia JN40, Paenibacillus sp. PALXIL05, Microbacterium sp. UYFA68, Chryseobacterium taiwanense DUCC3723, Brevundimonas sp. R3

Metabolites: cellulose (CHEBI:18246 · MediaIngredientMech · CMMMech), xylan (CHEBI:37166 · MediaIngredientMech · CMMMech), lignin (CHEBI:6457 · MediaIngredientMech · CMMMech)

Evidence

  • PMID:29392382 - SUPPORT (IN_VITRO)
    "A consortium containing Stenotrophomonas maltophilia, Paenibacillus sp., Microbacterium sp., Chryseobacterium taiwanense, and Brevundimonas sp. was found to be the most effective degrading synthetic community."
  • PMID:29392382 - SUPPORT (IN_VITRO)
    "Further work would be needed to understand the interactive relationship and metabolic pathways of these consortium partners."

Knowledge gaps & discussions (1)

KNOWLEDGE_GAP OPEN Which M48 partner activities account for the observed fraction changes?

Retain the M48 endpoint and zero arrows; remove two procedure nodes without restoring previously removed workflow arrows. Metabolic functional groups and isolate secretome assays guide selection but do not prove division of labor in M48. The50.6% value is a mean of three FTIR-derived fraction-change estimates, not50.6% each or gravimetric carbon loss. Table2 identification strings are 16S-based labels; exact isolate-to-culture/genome identity needs reconciliation. Main text and Tables2/3 read, not image-only Table1 cells, original figure pixels, supplementary files or raw measurements. #1564 separately tracks canonical labels, inoculum metadata and non-graph endpoint wording; approximately3e4 cells/mL per strain refers to the eighteen-strain control, not all five-member consortia.

Attaches to: ecological_interactions#MAMC-M48 Lignocellulose Degradation

Environmental Factors

Factor Value Unit
Substrate sugarcane waste N/A
Degradation efficiency 50.6% (96.5% of full consortium capacity) N/A
Selection method reductive screening N/A
Origin soil enrichment N/A

Growth Media

Mineral salts medium with sugarcane straw for lignocellulose degradation (CultureMech:000423)

View in CultureMech →

Temperature 28 degrees Celsius
Atmosphere AEROBIC

Composition

Component Concentration Unit CHEBI
Sugarcane straw (lignocellulose substrate) · MediaIngredientMech 10 g/L CHEBI:18246
Mineral salts medium base · MediaIngredientMech standard MSM formulation unspecified N/A
KNO3 · MediaIngredientMech 0.25 G_PER_L N/A
KH2PO4 · MediaIngredientMech 0.1 G_PER_L N/A
MgSO4 x 7 H2O · MediaIngredientMech 0.05 G_PER_L N/A
CaCl2 x 2 H2O · MediaIngredientMech 0.01 G_PER_L N/A
EDTA · MediaIngredientMech 5 G_PER_L N/A
CuCl2 x 5 H2O · MediaIngredientMech 0.1 G_PER_L N/A
FeSO4 x 7 H2O · MediaIngredientMech 2 G_PER_L N/A
ZnSO4 x 7 H2O · MediaIngredientMech 0.1 G_PER_L N/A
NiCl2 x 6 H2O · MediaIngredientMech 0.02 G_PER_L N/A
CoCl2 x 6 H2O · MediaIngredientMech 0.2 G_PER_L N/A
Na2MoO4 · MediaIngredientMech 0.03 G_PER_L N/A
MnCl2 x 4 H2O · MediaIngredientMech 0.03 G_PER_L N/A

Preparation notes: Mineral salts medium (MSM) supplemented with 1% sugarcane straw as lignocellulose substrate. All five strains are aerobic degraders isolated from soil through enrichment on sugarcane waste. Each strain inoculated at 1x10^5 cells/mL for total inoculum of 5x10^5 cells/mL. The consortium was optimized through reductive screening from 18 candidate strains using molecular phenotyping and metabolic characterization. MAMC-M48 (synthetic community 48) showed highest degradation efficiency (50.6% of all lignocellulose fractions), reaching 96.5% of the degradation rate observed with full 18-species pool.

Evidence

PMID:29392382

"Following an enrichment of soil bacteria capable of degrading lignocellulose material from sugarcane origin, we applied a reductive-screening approach based on molecular phenotyping, identification, and metabolic characterization to obtain a selection of 18 lignocellulose-degrading strains"

PMID:29392382

"The screening method described here successfully led to the selection of effective MAMC, whose degradation potential reached up 96.5% of the degradation rates when all 18 species were present"

PMID:29392382

"A consortium containing Stenotrophomonas maltophilia, Paenibacillus sp., Microbacterium sp., Chryseobacterium taiwanense, and Brevundimonas sp. was found to be the most effective degrading synthetic community"

Cross-Mech Suite Links

Explore related entities across the CultureBotAI X-Mech knowledge bases:

HabitatMech: soil ↗ TaxonMech: Stenotrophomonas maltophilia JN40 ↗ TraitMech: Stenotrophomonas maltophilia JN40 ↗ TaxonMech: Paenibacillus sp. PALXIL05 ↗ TraitMech: Paenibacillus sp. PALXIL05 ↗ TaxonMech: Microbacterium sp. UYFA68 ↗ TraitMech: Microbacterium sp. UYFA68 ↗ TaxonMech: Chryseobacterium taiwanense DUCC3723 ↗ TraitMech: Chryseobacterium taiwanense DUCC3723 ↗ TaxonMech: Brevundimonas sp. R3 ↗ TraitMech: Brevundimonas sp. R3 ↗ CultureMech Growth Media ↗ PathwayMech Pathways ↗