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Okeke-Lu Cellulolytic-Xylanolytic Consortium

Community ID CommunityMech:000048
Ecological State STABLE
Environment soil
ENVO:00001998

A defined 5-member bacterial consortium from Alabama soil capable of dual cellulose and hemicellulose degradation. The consortium consists of Pseudoxanthomonas byssovorax DB1, Microbacterium oxydans DB2, Bacillus sp. DB7, Ochrobactrum sp. DB8, and Klebsiella sp. DB13. Isolates DB1 and DB2 display the highest cellulase activity (27.83 and 31.22 U/mg), producing a complete enzyme system including filter paper cellulase, β-glucosidase, xylanase, and β-xylosidase for comprehensive lignocellulose bioprocessing.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Pseudoxanthomonas byssovorax DB1 NCBITaxon:324747
PRIMARY_DEGRADER
DOMINANT
Microbacterium oxydans DB2 NCBITaxon:82380
PRIMARY_DEGRADER
DOMINANT
Bacillus sp. DB7 NCBITaxon:870529
PRIMARY_DEGRADER
COMMON
Ochrobactrum sp. DB8 NCBITaxon:870532
PRIMARY_DEGRADER
COMMON
Klebsiella sp. DB13 NCBITaxon:870530
PRIMARY_DEGRADER
COMMON

Ecological Interactions

Ecological interaction network for Okeke-Lu Cellulolytic-Xylanolytic Consortium Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism

Cellulose Degradation

SYNTROPHY

Source Taxon: Pseudoxanthomonas byssovorax DB1

Target Taxon: Bacillus sp. DB7

Metabolites: cellulose (CHEBI:18246), glucose (CHEBI:17234)

Biological Processes:

Downstream Effects:
→ Hemicellulose Degradation

Evidence

  • PMID:20859703 - SUPPORT (IN_VITRO)
    "Use of microbial consortium in bioprocessing of lignocelluloses could reduce problems such as incomplete synergistic enzymes, end-product inhibition, adsorption, and requirement for high amounts of enzymes in direct use of enzymes."

Hemicellulose Degradation

SYNTROPHY

Source Taxon: Microbacterium oxydans DB2

Target Taxon: Klebsiella sp. DB13

Metabolites: xylan (CHEBI:37686), xylose (CHEBI:18222)

Biological Processes:

Evidence

  • PMID:20859703 - SUPPORT (IN_VITRO)
    "Isolate DB2 produced the highest xylanase activity (103.05 U mg⁻¹), while the highest β-xylosidase activity (7.72 U mg⁻¹) was observed with DB13"

Synergistic Lignocellulose Degradation

SYNTROPHY

Source Taxon: Bacillus sp. DB7

Target Taxon: Ochrobactrum sp. DB8

Biological Processes:

  • polysaccharide catabolic process (GO:0000272)
  • interspecies interaction between organisms (GO:0044419)

Evidence

  • PMID:20859703 - SUPPORT (IN_VITRO)
    "Use of microbial consortium in bioprocessing of lignocelluloses could reduce problems such as incomplete synergistic enzymes, end-product inhibition, adsorption, and requirement for high amounts of enzymes in direct use of enzymes."

Environmental Factors

Factor Value Unit
Source environment Alabama soil N/A
Substrate cellulose and hemicellulose N/A
Enzyme activity complete hydrolytic system N/A

Growth Media

Carboxymethyl cellulose mineral medium for cellulolytic enrichment (CultureMech:000423)

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Temperature 30 degrees Celsius
Atmosphere AEROBIC

Composition

Component Concentration Unit CHEBI
Carboxymethyl cellulose (sodium salt) 5 g/L CHEBI:31357
Xylan (hemicellulose substrate) 5 g/L CHEBI:37686
Mineral salts base standard formulation unspecified N/A
KNO3 0.25 G_PER_L N/A
KH2PO4 0.1 G_PER_L N/A
MgSO4 x 7 H2O 0.05 G_PER_L N/A
CaCl2 x 2 H2O 0.01 G_PER_L N/A
EDTA 5 G_PER_L N/A
CuCl2 x 5 H2O 0.1 G_PER_L N/A
FeSO4 x 7 H2O 2 G_PER_L N/A
ZnSO4 x 7 H2O 0.1 G_PER_L N/A
NiCl2 x 6 H2O 0.02 G_PER_L N/A
CoCl2 x 6 H2O 0.2 G_PER_L N/A
Na2MoO4 0.03 G_PER_L N/A
MnCl2 x 4 H2O 0.03 G_PER_L N/A

Preparation notes: Mineral salts medium supplemented with carboxymethyl cellulose (CMC) as cellulose substrate for enrichment and isolation of cellulolytic bacteria from Alabama soil. The consortium was composed from 19 purified monocultures evaluated for cellulolytic-xylanolytic potential, with 5 selected isolates (DB1, DB2, DB7, DB8, DB13) chosen based on high enzyme production. All strains are aerobic or facultatively anaerobic. Isolates DB1 and DB2 displayed the highest filter paper cellulase activity (27.83 and 31.22 U/mg). The defined consortium produces complete enzyme system: filter paper cellulase, beta-glucosidase, xylanase, and beta-xylosidase for comprehensive lignocellulose bioprocessing.

Evidence

PMID:20859703

"In an effort to mimic natural cellulolytic-xylanolytic microbial community in bioprocessing of lignocelluloses, we enriched cellulolytic-xylanolytic microorganisms, purified 19 monocultures and evaluated their cellulolytic-xylanolytic potential"

PMID:20859703

"Isolates DB1 and DB2 displayed the highest filter paper cellulase: 27.83 and 31.22 U mg⁻¹, respectively"

PMID:20859703

"Isolate DB2 produced the highest xylanase activity (103.05 U mg⁻¹), while the highest β-xylosidase activity (7.72 U mg⁻¹) was observed with DB13"