Ostreococcus-Dinoroseobacter B-Vitamin Mutualism

Community ID CommunityMech:000155

Ecological State STABLE

Environment laboratory marine phytoplankton coculture
ENVO:01001405

A defined marine algal-bacterial coculture in which the picoeukaryotic alga Ostreococcus tauri OTH95 and the roseobacter Dinoroseobacter shibae DF-12 form a reciprocal B-vitamin mutualism. D. shibae supplies cobalamin and thiamine needed by O. tauri, while O. tauri satisfies bacterial requirements for other B vitamins in a stable long-term coculture; the interaction is experimentally tractable under controlled light, temperature, and vitamin-amendment regimes.

Taxonomy

Taxon	Ontology ID	Functional Roles	Abundance
Ostreococcus tauri	NCBITaxon:70448	PRIMARY_PRODUCER CROSS_FEEDER	COMMON
PMID:30228381 - SUPPORT (IN_VITRO) "Ostreococcus tauri (OTH95)"
Dinoroseobacter shibae	NCBITaxon:215813	CROSS_FEEDER	COMMON
PMID:30228381 - SUPPORT (IN_VITRO) "Dinoroseobacter shibae DF-12"

Ecological Interactions

Taxon

Cross-feeding

Mutualism

Syntrophy

Competition

Commensalism

Niche partitioning

Colonization facilitation

Strain competition

Predation

Bacterial Rescue of Algal Cobalamin and Thiamine Auxotrophy

MUTUALISM

Source Taxon: Dinoroseobacter shibae

Target Taxon: Ostreococcus tauri

Metabolites: cobalamin (CHEBI:30411), thiamine (CHEBI:18385)

Biological Processes:

vitamin biosynthetic process (GO:0009110)
biological process involved in interspecies interaction between organisms (GO:0044419)

Evidence

PMID:30228381 - SUPPORT (IN_VITRO)

"B12 and B1 auxotrophy of O. tauri can be alleviated by co-culturing with a heterotrophic bacterial partner Dinoroseobacter shibae"

Algal Rescue of Bacterial Vitamin Requirements

MUTUALISM

Source Taxon: Ostreococcus tauri

Target Taxon: Dinoroseobacter shibae

Metabolites: biotin (CHEBI:15956)

Biological Processes:

vitamin biosynthetic process (GO:0009110)
biological process involved in interspecies interaction between organisms (GO:0044419)

Evidence

PMID:30228381 - SUPPORT (IN_VITRO)

"alga is in turn able to satisfy the reciprocal vitamin requirements of its bacterial partner in a stable long-term co-culture"

Photosynthate-Supported Roseobacter Association

CROSS_FEEDING

Source Taxon: Ostreococcus tauri

Target Taxon: Dinoroseobacter shibae

Biological Processes:

photosynthesis (GO:0015979)
biological process involved in interspecies interaction between organisms (GO:0044419)

Evidence

PMID:30228381 - SUPPORT (IN_VITRO)

"could supply thiamine and B12 to its hosts in exchange for a source of fixed carbon"

Associated Datasets

Dataset	Type	Repository	Accession
Ostreococcus-Dinoroseobacter B-vitamin rescue phenotyping Primary study reporting growth assays, repeated subculture phenotypes, and genomic pathway analysis for O. tauri OTH95 and D. shibae DF-12.	PHENOTYPE	OTHER	PMID:30228381

Environmental Factors

Factor	Value	Unit
B-vitamin availability	cobalamin, thiamine, biotin, niacin, and p-aminobenzoic acid amendments varied experimentally	N/A
PMID:30228381 - SUPPORT (IN_VITRO) "B-vitamins were added to cultures at the following concentrations"
light-dark cycle	16:8 h light:dark at 18 C	N/A
PMID:30228381 - SUPPORT (IN_VITRO) "grown at 18°C with a 16:8 h light:dark cycle"

Growth Media

L1 marine algal medium

Temperature 18 C

Atmosphere AEROBIC

Preparation notes: L1 medium was used for axenic O. tauri and cocultures containing O. tauri; vitamin additions were varied according to the experimental treatment.

Evidence

PMID:30228381

"All axenic and co-cultures containing O. tauri were grown at 18°C with a 16:8 h light:dark cycle"

PMID:30228381

"B-vitamins were added to cultures at the following concentrations: 0.40 nM cobalamin, 2.05 nM biotin, 296 nM thiamine"