PMI Variovorax Thermotolerance Collection

Community ID CommunityMech:000050

Ecological State ENGINEERED

Environment rhizosphere
ENVO:00005801

A 25-strain Variovorax collection from Populus roots, screened for bacterial effects on Arabidopsis thaliana thermotolerance using a rapid hydroponic assay. Six strains (CF313, YR634, GV004, GV035, YR752, OV084) significantly improve plant heat tolerance at 45C. CF313 was selected for detailed follow-up study. The thermal benefit requires direct contact with living bacteria; heat-killed bacteria and culture supernatants provide no protection.

Taxonomy

Taxon	Ontology ID	Functional Roles	Abundance
Variovorax sp. CF313	NCBITaxon:34072	PRIMARY_DEGRADER	N/A
PMID:37386471 - SUPPORT (IN_VIVO) "A follow-up study demonstrated the reproducibility of this assay and led to the discovery of a novel beneficial interaction"
Variovorax sp. YR634	NCBITaxon:34072	PRIMARY_DEGRADER	N/A
PMID:37386471 - SUPPORT (IN_VIVO) "method was used to screen 25 strains of the plant growth promoting Variovorax spp. for enhanced plant thermotolerance"
Variovorax sp. GV004	NCBITaxon:34072	PRIMARY_DEGRADER	N/A
PMID:37386471 - SUPPORT (IN_VIVO) "method was used to screen 25 strains of the plant growth promoting Variovorax spp. for enhanced plant thermotolerance"
Variovorax sp. GV035	NCBITaxon:34072	PRIMARY_DEGRADER	N/A
PMID:37386471 - SUPPORT (IN_VIVO) "method was used to screen 25 strains of the plant growth promoting Variovorax spp. for enhanced plant thermotolerance"
Variovorax sp. YR752	NCBITaxon:34072	PRIMARY_DEGRADER	N/A
PMID:37386471 - SUPPORT (IN_VIVO) "method was used to screen 25 strains of the plant growth promoting Variovorax spp. for enhanced plant thermotolerance"
Variovorax sp. OV084	NCBITaxon:34072	PRIMARY_DEGRADER	N/A
PMID:37386471 - SUPPORT (IN_VIVO) "method was used to screen 25 strains of the plant growth promoting Variovorax spp. for enhanced plant thermotolerance"

Ecological Interactions

Taxon

Cross-feeding

Mutualism

Syntrophy

Competition

Commensalism

Niche partitioning

Colonization facilitation

Strain competition

Predation

Bacterially-mediated plant thermotolerance

MUTUALISM

Source Taxon: Variovorax

Target Taxon: Arabidopsis thaliana

Biological Processes:

response to heat (GO:0009408)

Evidence

PMID:37386471 - SUPPORT (IN_VIVO)

"A follow-up study demonstrated the reproducibility of this assay and led to the discovery of a novel beneficial interaction"
PMID:37386471 - SUPPORT (IN_VIVO)

"The throughput and reproducibility of the system is ideal for testing many genetic variants of Arabidopsis and bacterial strains"

External Resources

Name	Repository	Resource ID
Primary publication A rapid assay for assessing bacterial effects on Arabidopsis thermotolerance. Lee et al. 2023, Plant Methods.	OTHER	PMID:37386471
PMID:37386471 - SUPPORT (IN_VIVO) "method was used to screen 25 strains of the plant growth promoting Variovorax spp. for enhanced plant thermotolerance"
Climate-driven plant-microbiome study Climate-driven divergence in plant-microbiome interactions generates range-wide variation in bud break phenology. Weston, Carrell et al. 2021, Communications Biology.	OTHER	PMID:34135464

Environmental Factors

Factor	Value	Unit
Heat shock temperature	45	degrees Celsius
PMID:37386471 - SUPPORT (IN_VIVO) "method was used to screen 25 strains of the plant growth promoting Variovorax spp. for enhanced plant thermotolerance"