A defined five-strain artificial microbial consortium constructed to degrade the antibiotic pleuromutilin, assembled from bacteria isolated out of a natural consortium (En4) that was itself developed by gradient enrichment and domestication under pleuromutilin stress. The consortium comprises Stenotrophomonas pavanii, Stenotrophomonas maltophilia, Lysinibacillus sphaericus, Lysinibacillus mangiferihumi, and Pseudomonas songnenensis, and achieves a pleuromutilin degradation rate of 91.97%. In the parent natural community, pleuromutilin stress reduced microbial diversity and selected core taxa with functional specialization (notably Proteobacteria, and enriched pollutant-degrading genera such as Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium and Cupriavidus), and potential degradation products and metabolic pathways were proposed for the artificial consortium.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Stenotrophomonas pavanii | NCBITaxon:487698 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
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N/A |
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| Stenotrophomonas maltophilia | NCBITaxon:40324 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
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N/A |
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| Lysinibacillus sphaericus | NCBITaxon:1421 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
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N/A |
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| Lysinibacillus mangiferihumi | NCBITaxon:1130819 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
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N/A |
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| Pseudomonas songnenensis | NCBITaxon:1176259 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
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N/A |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Ninety-Two-Percent Pleuromutilin Degradation
The defined five-strain artificial consortium achieved reported pleuromutilin degradation of 91.97%. Preserve this positive community-level endpoint without asserting product-removal cooperation, partner-specific flux, complete mineralization or a superiority comparison not stated in the accessible abstract. SYNTROPHY does not require obligacy, but a measured community degradation endpoint is not itself the product-removal mechanism.
Participating Taxa: Stenotrophomonas pavanii, Stenotrophomonas maltophilia, Lysinibacillus sphaericus, Lysinibacillus mangiferihumi, Pseudomonas songnenensis
Metabolites: pleuromutilin (CHEBI:8269 · MediaIngredientMech · CMMMech)
Evidence
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PMID:42133144 - SUPPORT (IN_VITRO)"was constructed, achieving a degradation rate of 91.97%"
Proposed Artificial-Consortium Degradation Products and Pathways
HYPOTHESIZED: metabolomic profiling of the artificial five-strain consortium motivated proposed pleuromutilin degradation products and pathways. Public Supplement Table S3 compares pleuromutilin and glucose conditions by untargeted metabolomics. Differential abundance and database spectral assignments do not alone establish that every metabolite derives from pleuromutilin or identify a strain-resolved transfer route.
Participating Taxa: Stenotrophomonas pavanii, Stenotrophomonas maltophilia, Lysinibacillus sphaericus, Lysinibacillus mangiferihumi, Pseudomonas songnenensis
Metabolites: pleuromutilin (CHEBI:8269 · MediaIngredientMech · CMMMech)
Evidence
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PMID:42133144 - SUPPORT (IN_VITRO)"The metabolic profile of the artificial microbial consortia during the degradation of pleuromutilin were studied, and potential degradation products and metabolic pathways were proposed "
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which compatibility and degradation mechanisms belong to the defined five-strain community?
Natural En4 enrichment showed reduced diversity and enriched pollutant-associated genera; preserve those positives as parent-community context, not interactions among the five selected strains. Assembly/isolation workflow remains in the record description. Public Table S1 lists bacterial and fungal isolates and identifies W12, rather than same-species W10, as the selected Pseudomonas songnenensis in Table S2. Table S2 reports no inhibition zones, not cross-feeding or long-term stability. Table S3 supports differential metabolomics, not compound provenance or partner flux. Main text remains inaccessible; source DOCX tables were read by OOXML, not rendered pages. Missing compatibility/pathway topology remains #1715 pending a reviewed, explicitly authorized Edison report; no new structure is added. Non-graph isolate and strain identity remains #1716. The local SYNTROPHY definition does not assert obligacy. The retained outcome and proposed-products nodes remain untyped because they do not themselves establish product-removal feedback, not because obligatory dependence is unproven.
Attaches to: ecological_interactions#Ninety-Two-Percent Pleuromutilin Degradation, ecological_interactions#Proposed Artificial-Consortium Degradation Products and Pathways
2 evidence item(s)
pleuromutilin stress significantly reduced microbial diversity, and core communities with functional specialization were identified across multiple taxa
Supports pleuromutilin-associated diversity loss and functional specialization across core taxa in the natural consortium.
The metabolic profile of the artificial microbial consortia during the degradation of pleuromutilin were studied, and potential degradation products and metabolic pathways were proposed
Supports proposed products/pathways, not experimentally resolved interspecies exchange or product-removal feedback on upstream metabolism.
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