A defined five-strain artificial microbial consortium constructed to degrade the antibiotic pleuromutilin, assembled from bacteria isolated out of a natural consortium (En4) that was itself developed by gradient enrichment and domestication under pleuromutilin stress. The consortium comprises Stenotrophomonas pavanii, Stenotrophomonas maltophilia, Lysinibacillus sphaericus, Lysinibacillus mangiferihumi, and Pseudomonas songnenensis, and achieves a pleuromutilin degradation rate of 91.97%. In the parent natural community, pleuromutilin stress reduced microbial diversity and selected core taxa with functional specialization (notably Proteobacteria, and enriched pollutant-degrading genera such as Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium and Cupriavidus), and potential degradation products and metabolic pathways were proposed for the artificial consortium.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Stenotrophomonas pavanii | NCBITaxon:487698 |
PRIMARY_DEGRADER
|
N/A |
|
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| Stenotrophomonas maltophilia | NCBITaxon:40324 |
PRIMARY_DEGRADER
|
N/A |
|
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| Lysinibacillus sphaericus | NCBITaxon:1421 |
PRIMARY_DEGRADER
|
N/A |
|
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| Lysinibacillus mangiferihumi | NCBITaxon:1130819 |
PRIMARY_DEGRADER
|
N/A |
|
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| Pseudomonas songnenensis | NCBITaxon:1176259 |
PRIMARY_DEGRADER
|
N/A |
|
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Ecological Interactions
Cooperative pleuromutilin biodegradation by the artificial consortium
NICHE_PARTITIONINGMetabolites: pleuromutilin (CHEBI:8269)
Evidence
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PMID:42133144 - SUPPORT (IN_VITRO)"an efficient artificial microbial consortium comprising five strains: Stenotrophomonas pavanii, Stenotrophomonas maltophilia, Lysinibacillus sphaericus, Lysinibacillus mangiferihumi, and Pseudomonas songnenensis, was constructed, achieving a degradation rate of 91.97%."
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PMID:42133144 - SUPPORT (OTHER)"core communities with functional specialization were identified across multiple taxa, primarily at the level of Proteobacteria"