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Pseudomonas-Pedobacter Social Spreading Coculture

Community ID CommunityMech:000161
Ecological State ENGINEERED
Environment soil
ENVO:00001998

A two-species soil-bacterial model community composed of Pseudomonas fluorescens Pf0-1 and Pedobacter sp. V48. When mixed on low-nutrient, high-salt hard agar, the two sessile monocultures produce an emergent interspecies social spreading phenotype in which both bacteria comigrate across the agar surface. The system is used to study contact-dependent and environment-dependent emergent motility in small soil microbial communities.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Pseudomonas fluorescens Pf0-1 NCBITaxon:294
CROSS_FEEDER
N/A
  • PMID:30700513 - SUPPORT (IN_VITRO)
    "Pseudomonas fluorescens Pf0-1 and Pedobacter sp. V48"
  • PMID:34288708 - SUPPORT (IN_VITRO)
    "Pseudomonas fluorescens Pf0-1 and Pedobacter sp. V48 engage in interspecies social spreading"
Pedobacter sp. V48 NCBITaxon:84567
CROSS_FEEDER
N/A
  • PMID:30700513 - SUPPORT (IN_VITRO)
    "Pseudomonas fluorescens Pf0-1 and Pedobacter sp. V48"
  • PMID:34288708 - SUPPORT (IN_VITRO)
    "Pseudomonas fluorescens Pf0-1 and Pedobacter sp. V48 interact"

Ecological Interactions

Ecological interaction network for Pseudomonas-Pedobacter Social Spreading Coculture Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Interspecies Social Spreading

COLONIZATION_FACILITATION

Source Taxon: Pedobacter sp. V48

Target Taxon: Pseudomonas fluorescens Pf0-1

Biological Processes:

  • locomotion (GO:0040011)
  • biological process involved in interspecies interaction between organisms (GO:0044419)

Evidence

  • PMID:30700513 - SUPPORT (IN_VITRO)
    "coculture reveals an emergent phenotype that we term “interspecies social spreading,” where the mixed colony spreads across the hard surface"

Pseudomonas-Pedobacter Comigration

MUTUALISM

Source Taxon: Pseudomonas fluorescens Pf0-1

Target Taxon: Pedobacter sp. V48

Biological Processes:

  • locomotion (GO:0040011)
  • biological process involved in interspecies interaction between organisms (GO:0044419)

Evidence

  • PMID:30700513 - SUPPORT (IN_VITRO)
    "Both species remain associated throughout the spreading colony"
  • PMID:30700513 - SUPPORT (IN_VITRO)
    "we recovered both species from each point in the spreading colony"

Contact-Dependent Initiation

COLONIZATION_FACILITATION

Source Taxon: Pedobacter sp. V48

Target Taxon: Pseudomonas fluorescens Pf0-1

Biological Processes:

  • cell communication (GO:0007154)
  • biological process involved in interspecies interaction between organisms (GO:0044419)

Evidence

  • PMID:30700513 - SUPPORT (IN_VITRO)
    "initiation of social spreading requires close association between the two species of bacteria"

Associated Datasets

Dataset Type Repository Accession
Exact-composition publication - Pseudomonas-Pedobacter social spreading
Primary publication defining the Pf0-1 plus V48 two-species community and its emergent interspecies social spreading phenotype. 		PHENOTYPE OTHER PMID:30700513
Exact-composition publication - prevalence and variation of social spreading
Follow-up study that revisits the exact Pf0-1 plus V48 pair while testing whether social spreading is conserved among related Pseudomonas fluorescens and Pedobacter isolates. 		PHENOTYPE OTHER PMID:34288708

Environmental Factors

Factor Value Unit
low-nutrient high-salt hard agar TSB-NK medium with 2% agar, requiring low nutrient and high NaCl conditions for Pf0-1 plus V48 social spreading N/A
  • PMID:30700513 - SUPPORT (IN_VITRO)
    "low-nutrient conditions are insufficient to promote social spreading without high salt concentrations"
  • PMID:34288708 - SUPPORT (IN_VITRO)
    "While Pf0-1+V48 require low nutrient but high NaCl conditions"
hard agar surface 2% agar surface not permissive for monoculture motility N/A
  • PMID:30700513 - SUPPORT (IN_VITRO)
    "On hard agar, which is not permissive for motility of the monoculture of either species"

Growth Media

TSB-NK hard agar social spreading medium

Temperature 25 C
Atmosphere AEROBIC

Preparation notes: Low-nutrient, high-NaCl TSB-NK medium solidified with 2% agar; mixed Pf0-1 and V48 colonies were incubated on the agar surface to assay spreading.

Evidence

PMID:30700513

"TSB-NK medium solidified with 2% agar"