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Pseudomonas stutzeri + Rhodococcus Naphthalene-degrading Biochar-bridged Engineered Consortium

Community ID CommunityMech:000291
Ecological State ENGINEERED
Environment engineered consortium immobilized on S-N-doped biochar (naphthalene bioremediation reactor)
ENVO:00002123

Two-member engineered bioremediation consortium in which genetically engineered Pseudomonas stutzeri (overexpressing nahAc and nahB) and a Rhodococcus strain (overexpressing nahH and catA) are co-anchored on S-N-doped biochar to mineralize naphthalene. Pseudomonas stutzeri acts as an oxidative bio-anode that initiates naphthalene degradation and releases electrons, while the conductive biochar matrix mediates direct interspecies electron transfer (DIET) and extracellular electron transfer (EET) to Rhodococcus, which serves as a reductive bio-cathode driving oxygenolytic ring-cleavage catalysis. The resulting bioelectrochemical syntrophic circuit achieved >98.3% naphthalene removal and 89.5% mineralization by circumventing diffusive bottlenecks.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Pseudomonas stutzeri (overexpressing nahAc and nahB) NCBITaxon:316
PRIMARY_DEGRADER ELECTRON_DONOR ELECTROGEN SYNTROPHIC_PARTNER
N/A
  • PMID:42139785 - SUPPORT (IN_VITRO)
    "co-anchoring engineered Pseudomonas stutzeri (overexpressing nahAc and nahB)"
Rhodococcus rhodococcus (overexpressing nahH and catA) NCBITaxon:1827
PRIMARY_DEGRADER ELECTRON_ACCEPTOR ELECTROTROPH SYNTROPHIC_PARTNER
N/A
  • PMID:42139785 - SUPPORT (IN_VITRO)
    "Rhodococcus rhodococcus (overexpressing nahH and catA)"

Ecological Interactions

Ecological interaction network for Pseudomonas stutzeri + Rhodococcus Naphthalene-degrading Biochar-bridged Engineered Consortium Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Biochar-bridged direct interspecies electron transfer (DIET)

SYNTROPHY

Source Taxon: Pseudomonas stutzeri (overexpressing nahAc and nahB)

Target Taxon: Rhodococcus rhodococcus (overexpressing nahH and catA)

Evidence

  • PMID:42139785 - SUPPORT (IN_VITRO)
    "facilitating direct interspecies electron transfer (DIET) and enhancing extracellular electron transfer (EET)"

Syntrophic naphthalene mineralization circuit

SYNTROPHY

Source Taxon: Pseudomonas stutzeri (overexpressing nahAc and nahB)

Target Taxon: Rhodococcus rhodococcus (overexpressing nahH and catA)

Metabolites: naphthalene (CHEBI:16482)

Evidence

  • PMID:42139785 - SUPPORT (IN_VITRO)
    "Electrochemical and multi-omics analyses confirmed the establishment of this syntrophic circuit, demonstrating elevated electron flux, coordinated upregulation of catabolic genes"