Two-member engineered bioremediation consortium in which genetically engineered Pseudomonas stutzeri (overexpressing nahAc and nahB) and a Rhodococcus strain (overexpressing nahH and catA) are co-anchored on S-N-doped biochar to mineralize naphthalene. Pseudomonas stutzeri acts as an oxidative bio-anode that initiates naphthalene degradation and releases electrons, while the conductive biochar matrix mediates direct interspecies electron transfer (DIET) and extracellular electron transfer (EET) to Rhodococcus, which serves as a reductive bio-cathode driving oxygenolytic ring-cleavage catalysis. The resulting bioelectrochemical syntrophic circuit achieved >98.3% naphthalene removal and 89.5% mineralization by circumventing diffusive bottlenecks.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Pseudomonas stutzeri (overexpressing nahAc and nahB) — ontology label: Stutzerimonas stutzeri | NCBITaxon:316 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
ELECTRON_DONOR
ELECTROGEN
SYNTROPHIC_PARTNER
|
N/A |
|
|||
| Rhodococcus rhodococcus (overexpressing nahH and catA) — ontology label: Rhodococcus <high G+C Gram-positive bacteria> | NCBITaxon:1827 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
ELECTRON_ACCEPTOR
ELECTROTROPH
SYNTROPHIC_PARTNER
|
N/A |
|
|||
Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Biochar-bridged direct interspecies electron transfer (DIET)
SYNTROPHYPARTIAL: the authors interpret the conductive material as coupling donor electron release with recipient electron use in a syntrophic model. Retain this reported coupling type, which does not assert obligacy. Electrochemical and multi-omics support do not independently isolate the interspecies route from other material-associated effects in the accessible abstract.
Source Taxon: Pseudomonas stutzeri (overexpressing nahAc and nahB)
Target Taxon: Rhodococcus rhodococcus (overexpressing nahH and catA)
Biological Processes:
- electron transport chain (GO:0022900 · PathwayMech)
PARTIAL: the reported conductive interface is proposed to couple the partners within the authors degradation-circuit model; route-specific mediation is not independently resolved by the accessible abstract.
Evidence
-
PMID:42139785 - SUPPORT (IN_VITRO)"facilitating direct interspecies electron transfer (DIET) and enhancing extracellular electron transfer (EET)"
Syntrophic naphthalene mineralization circuit
SYNTROPHYPARTIAL: the authors interpret coordinated electron flux and catabolic responses as a syntrophic degradation circuit. Retain that reported model with canonical participants. The local interaction type does not assert obligacy, and the accessible abstract does not independently isolate every mediator.
Participating Taxa: Pseudomonas stutzeri (overexpressing nahAc and nahB), Rhodococcus rhodococcus (overexpressing nahH and catA)
Metabolites: naphthalene (CHEBI:16482 · MediaIngredientMech · CMMMech)
PARTIAL: the proposed coordinated circuit is offered as an explanation for the reported removal and mineralization endpoints. The abstract does not isolate each mediator from concurrent treatment effects.
Evidence
-
PMID:42139785 - SUPPORT (IN_VITRO)"Electrochemical and multi‑omics analyses confirmed the establishment of this syntrophic circuit, demonstrating elevated electron flux, coordinated upregulation of catabolic genes"
Enhanced Naphthalene Removal and Mineralization
The source reports more than 98.3% naphthalene removal and 89.5% mineralization. Preserve these separate positive endpoints without treating the measured outcome itself as an electron-transfer interaction or independently auditing a complete carbon balance.
Participating Taxa: Pseudomonas stutzeri (overexpressing nahAc and nahB), Rhodococcus rhodococcus (overexpressing nahH and catA)
Metabolites: naphthalene (CHEBI:16482 · MediaIngredientMech · CMMMech)
Evidence
-
PMID:42139785 - SUPPORT (IN_VITRO)"The resulting bioelectrochemical synergy achieved exceptional naphthalene removal (>98.3%) and mineralization efficiency (89.5%)"
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN What distinguishes the reported electron-transfer model from the treatment endpoints?
All three nodes and both existing directions remain. The authors electron-transfer model retains its SYNTROPHY classification with PARTIAL evidence bounds: the local enum does not require obligacy. The community removal/mineralization endpoint is distinct from the coupling mechanism. Only the fresh primary abstract was retrieved; unavailable methods are not proof that route-specific controls were absent. Canonical source labels are preserved, not newly certified strain identities. No new genetic designs, optimization details or experimental procedures were added.
Attaches to: ecological_interactions#Biochar-bridged direct interspecies electron transfer (DIET), ecological_interactions#Syntrophic naphthalene mineralization circuit, ecological_interactions#Enhanced Naphthalene Removal and Mineralization
2 evidence item(s)
facilitating direct interspecies electron transfer (DIET) and enhancing extracellular electron transfer (EET)
Supports the authors DIET/EET interpretation, not independent resolution of the electron-transfer route or obligatory mutual dependence.
The resulting bioelectrochemical synergy achieved exceptional naphthalene removal (>98.3%) and mineralization efficiency (89.5%)
Supports the measured removal and mineralization endpoint of the engineered consortium.
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