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Saccharomyces-Acinetobacter Lignocellulose Hydrolysate Detoxification Coculture

Community ID CommunityMech:000263
Ecological State ENGINEERED
Environment synthetic lignocellulosic hydrolysate laboratory coculture
ENVO:01001405

A defined two-member synthetic coculture pairing engineered Saccharomyces cerevisiae and engineered Acinetobacter baylyi ADP1 for upgrading a synthetic lignocellulosic hydrolysate. A. baylyi ADP1 bioconverts the furan aldehyde inhibitors furfural and 5-hydroxymethylfurfural that are present in lignocellulose hydrolysates without competing with S. cerevisiae for substrates, while also redirecting S. cerevisiae's residual carbon sources and byproducts into wax esters. The detoxification function raises S. cerevisiae's lactic acid productivity approximately 1.5-fold compared to a monoculture, making the coculture a candidate platform for inhibitor-tolerant lignocellulose conversion.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
engineered Saccharomyces cerevisiae NCBITaxon:4932
SECONDARY_FERMENTER
COMMON
  • PMID:38711153 - SUPPORT (IN_VITRO)
    "The lactic acid productivity of S. cerevisiae was improved approximately 1.5-fold"
engineered Acinetobacter baylyi ADP1 NCBITaxon:202950
CROSS_FEEDER
COMMON
  • PMID:38711153 - SUPPORT (IN_VITRO)
    "A. baylyi ADP1 showed efficient bioconversion of furan aldehydes present in the hydrolysate, namely furfural and 5-hydroxymethylfurfural"

Ecological Interactions

Ecological interaction network for Saccharomyces-Acinetobacter Lignocellulose Hydrolysate Detoxification Coculture Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Furan Aldehyde Detoxification Cross-Protection

COMMENSALISM

Source Taxon: engineered Acinetobacter baylyi ADP1

Target Taxon: engineered Saccharomyces cerevisiae

Metabolites: furfural (CHEBI:34768), 5-hydroxymethylfurfural (CHEBI:34717)

Biological Processes:

Evidence

  • PMID:38711153 - SUPPORT (IN_VITRO)
    "A. baylyi ADP1 showed efficient bioconversion of furan aldehydes present in the hydrolysate, namely furfural and 5-hydroxymethylfurfural, and did not compete for substrates with S. cerevisiae"
  • PMID:38711153 - SUPPORT (IN_VITRO)
    "The lactic acid productivity of S. cerevisiae was improved approximately 1.5-fold (to 0.41 ± 0.08 g/L/h) in the coculture with A. baylyi ADP1, compared to a monoculture of S. cerevisiae"

Carbon Diversion to Wax Esters

CROSS_FEEDING

Source Taxon: engineered Saccharomyces cerevisiae

Target Taxon: engineered Acinetobacter baylyi ADP1

Biological Processes:

Evidence

  • PMID:38711153 - SUPPORT (IN_VITRO)
    "the remaining carbon sources and byproducts of S. cerevisiae were directed to wax ester production by A. baylyi ADP1"

Environmental Factors

Factor Value Unit
Furan aldehyde inhibitors in synthetic lignocellulosic hydrolysate furfural and 5-hydroxymethylfurfural present N/A
  • PMID:38711153 - SUPPORT (IN_VITRO)
    "the presence of inhibitory compounds, such as furan aldehydes"