A defined two-member synthetic coculture pairing engineered Saccharomyces cerevisiae and engineered Acinetobacter baylyi ADP1 for upgrading a synthetic lignocellulosic hydrolysate. A. baylyi ADP1 bioconverts the furan aldehyde inhibitors furfural and 5-hydroxymethylfurfural that are present in lignocellulose hydrolysates without competing with S. cerevisiae for substrates, while also redirecting S. cerevisiae's residual carbon sources and byproducts into wax esters. The detoxification function raises S. cerevisiae's lactic acid productivity approximately 1.5-fold compared to a monoculture, making the coculture a candidate platform for inhibitor-tolerant lignocellulose conversion.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| engineered Saccharomyces cerevisiae — ontology label: Saccharomyces cerevisiae | NCBITaxon:4932 · TaxonMech · TraitMech |
SECONDARY_FERMENTER
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COMMON |
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| engineered Acinetobacter baylyi ADP1 — ontology label: Acinetobacter baylyi | NCBITaxon:202950 · TaxonMech · TraitMech |
CROSS_FEEDER
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COMMON |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Furan Aldehyde Detoxification Cross-Protection
A. baylyi ADP1 bioconverts the furan aldehydes faster than the yeast in the reported strain comparisons, and coculture has faster inhibitor disappearance and greater lactic acid productivity than the yeast monoculture. PARTIAL: detoxification is the authors' proposed mediator of that benefit, not an independently isolated quantitative contribution. The benefit does not establish neutral fitness of ADP1; the authors describe the overall consortium as facultative mutualism, with both strains able to grow independently.
Source Taxon: engineered Acinetobacter baylyi ADP1
Target Taxon: engineered Saccharomyces cerevisiae
Metabolites: furfural (CHEBI:34768 · MediaIngredientMech · CMMMech), 5-hydroxymethylfurfural (CHEBI:412516 · MediaIngredientMech · CMMMech)
Biological Processes:
- response to xenobiotic stimulus (GO:0009410 · PathwayMech)
PARTIAL: faster inhibitor conversion is proposed to relieve stress and support the higher coculture lactic acid productivity. The coculture comparison supports a net benefit but does not isolate detoxification from all other partner effects or prove a higher lactic acid yield.
Evidence
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PMID:38711153 - SUPPORT (IN_VITRO)"A. baylyi ADP1 showed efficient bioconversion of furan aldehydes present in the hydrolysate, namely furfural and 5-hydroxymethylfurfural, and did not compete for substrates with S. cerevisiae"
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PMID:38711153 - SUPPORT (IN_VITRO)"The lactic acid productivity of S. cerevisiae was improved approximately 1.5-fold (to 0.41 ± 0.08 g/L/h) in the coculture with A. baylyi ADP1, compared to a monoculture of S. cerevisiae"
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PMID:38711153 - SUPPORT (IN_VITRO)"both strains are able to independently grow in the SLH medium"
Carbon Diversion to Wax Esters
CROSS_FEEDINGPARTIAL: residual medium substrates and yeast-derived byproducts can contribute carbon used by ADP1 for wax ester production. Unconsumed feedstock is not itself a metabolite secreted by yeast, and the precise contribution of yeast-derived carbon is not traced. The primary results reliably quantified wax esters in only one of four coculture replicates, so the positive observation is retained without claiming a reproducible second product stream.
Source Taxon: engineered Saccharomyces cerevisiae
Target Taxon: engineered Acinetobacter baylyi ADP1
Biological Processes:
- wax biosynthetic process (GO:0010025 · PathwayMech)
PARTIAL: ADP1 carbon use provides a possible second product stream alongside yeast lactic acid production, but reliable wax ester quantification occurred in only one coculture replicate. The direction represents a supported production interpretation with unresolved carbon provenance and reproducibility.
Evidence
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PMID:38711153 - SUPPORT (IN_VITRO)"the remaining carbon sources and byproducts of S. cerevisiae were directed to wax ester production by A. baylyi ADP1"
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PMID:38711153 - SUPPORT (IN_VITRO)"Measurable amounts of WEs were only detected in one of the cocultures"
Simultaneous Detoxification and Product Formation
The two-member synthetic-hydrolysate coculture showed faster inhibitor conversion and approximately 1.5-fold greater lactic acid productivity than the yeast monoculture. Lactic acid yield was similar, and wax esters were reliably quantified in only one coculture replicate. This is a community process outcome, not by itself a pairwise metabolite-exchange event or evidence of reproducible simultaneous product yields from real industrial hydrolysates.
Participating Taxa: engineered Saccharomyces cerevisiae, engineered Acinetobacter baylyi ADP1
Metabolites: furfural (CHEBI:34768 · MediaIngredientMech · CMMMech), 5-hydroxymethylfurfural (CHEBI:412516 · MediaIngredientMech · CMMMech)
Biological Processes:
- response to xenobiotic stimulus (GO:0009410 · PathwayMech)
Evidence
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PMID:38711153 - SUPPORT (IN_VITRO)"simultaneous inhibitor detoxification and production of lactic acid and wax esters"
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PMID:38711153 - SUPPORT (IN_VITRO)"Measurable amounts of WEs were only detected in one of the cocultures"
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN How reproducible is wax-ester coproduction and how much of the yeast benefit is specifically mediated by detoxification?
Primary results support faster inhibitor conversion and higher lactic acid productivity, not a higher lactic acid yield or neutral ADP1 fitness. Wax esters were reliably quantified in only one of four coculture replicates, and residual feedstock must be distinguished from yeast-produced carbon. The authors' facultative-mutualism interpretation does not demonstrate obligate dependence. Preserve all three nodes and both PARTIAL directions; no new culture, strain-design or optimization instructions are added.
Attaches to: ecological_interactions#Furan Aldehyde Detoxification Cross-Protection, ecological_interactions#Carbon Diversion to Wax Esters, ecological_interactions#Simultaneous Detoxification and Product Formation
1 evidence item(s)
Measurable amounts of WEs were only detected in one of the cocultures
Bounds the reproducibility of the positive wax-ester result.
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Furan aldehyde inhibitors in synthetic lignocellulosic hydrolysate | furfural and 5-hydroxymethylfurfural present | N/A |
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Growth Media
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