A defined three-species anode-associated exoelectrogenic biofilm community composed of Shewanella oneidensis, Geobacter sulfurreducens, and Geobacter metallireducens. The community was assembled to study biofilm formation dynamics, strain interactions, redox potential resilience, and electron transfer activity in bioelectrochemical systems.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Shewanella oneidensis | NCBITaxon:70863 · TaxonMech · TraitMech |
SYNTROPHIC_PARTNER
CROSS_FEEDER
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ABUNDANT |
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| Geobacter sulfurreducens | NCBITaxon:35554 · TaxonMech · TraitMech |
SYNTROPHIC_PARTNER
CROSS_FEEDER
|
ABUNDANT |
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| Geobacter metallireducens | NCBITaxon:28232 · TaxonMech · TraitMech |
SYNTROPHIC_PARTNER
CROSS_FEEDER
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ABUNDANT |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Three-Species Anode Biofilm Formation
The three-member culture formed a stable anode-associated biofilm under the tested bioelectrochemical conditions. Coexistence on the electrode supports this community observation, but does not by itself establish that one member facilitated another's colonization or that every member contributed equally.
Biological Processes:
- biofilm formation (GO:0042710 · PathwayMech)
PARTIAL: the anode-associated community provides the setting for the measured mixed-culture electron transfer. The study does not independently isolate biofilm formation or architecture as the cause of the current increment over solitary cultures.
Evidence
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PMID:28087529 - SUPPORT (IN_VITRO)"organisms build a stable biofilm on an electrode surface"
Coculture-Associated Metabolic Responses
Shewanella oneidensis and Geobacter sulfurreducens upregulated central-metabolism and electron-transfer-associated transcripts in the three-species coculture comparison. This supports the authors' positive-interaction hypothesis, but is not a two-member perturbation experiment or a direct demonstration of reciprocal fitness benefit.
Participating Taxa: Shewanella oneidensis, Geobacter sulfurreducens
HYPOTHESIZED: the coculture-associated metabolic responses may contribute to increased electron transfer. Transcript abundance and current covary, but the proposed interaction pathways were not individually perturbed to establish their causal contribution.
Evidence
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PMID:28087529 - SUPPORT (IN_VITRO)"Transcriptomic results point toward a positive interaction among the individual strains"
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PMID:28087529 - SUPPORT (IN_VITRO)"S. oneidensis and G. sulfurreducens upregulated their central metabolisms"
Higher Current in the Three-Species Coculture
The three-species coculture produced more current than each solitary culture in the study-specific reactor and feeding comparisons. Retain this measured positive community result without treating it as proof of superadditivity, reciprocal fitness benefit or a resolved syntrophic product-removal feedback. The proposed interaction mechanisms remain distinct from the electrical endpoint.
Evidence
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PMID:28087529 - SUPPORT (IN_VITRO)"During solitary growth, each of the strains produced less current than that from coculture conditions"
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PMID:28087529 - SUPPORT (IN_VITRO)"positive interactions that might lead to accelerated electron transfer"
Associated Datasets
| Dataset | Type | Repository | Accession |
|---|---|---|---|
|
GSE79750 exoelectrogenic biofilm transcriptome dataset
GEO Series for transcriptomic measurements from the multispecies exoelectrogenic biofilm community in bioelectrochemical systems. |
METATRANSCRIPTOMICS | OTHER | GSE79750 |
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which proposed interactions causally explain the measured coculture current advantage?
The current comparison and transcriptomic responses are real observations. Hydrogen exchange, shared flavin use and propionate-related benefits remain proposed mechanisms with different degrees of support; they do not establish reciprocal fitness or measured product-removal feedback. Preserve both existing directions with explicit uncertainty instead of assigning mutualism or syntrophy to the aggregate endpoint.
Attaches to: ecological_interactions#Three-Species Anode Biofilm Formation, ecological_interactions#Coculture-Associated Metabolic Responses, ecological_interactions#Higher Current in the Three-Species Coculture
1 evidence item(s)
positive interactions that might lead to accelerated electron transfer
Supports the authors' proposed positive-interaction mechanism, not an individually isolated mediator.
External Resources
| Name | Repository | Resource ID |
|---|---|---|
|
Exact-system primary publication - three-species exoelectrogenic biofilm
Primary publication reporting the defined S. oneidensis, G. sulfurreducens, and G. metallireducens anode-associated exoelectrogenic biofilm community. |
OTHER | PMID:28087529 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Electrode surface biofilm habitat | anode-associated biofilm | N/A |
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| Applied anode redox potential | maximum electron transfer above 0.04 V versus normal hydrogen electrode | N/A |
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| Redox-potential resilience | resilient to anode redox-potential changes | N/A |
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