Shewanella oneidensis + Pseudomonas aeruginosa Fe0-dependent Electro-syntrophic Denitrifying Consortium

Community ID CommunityMech:000288

Ecological State ENGINEERED

Environment anaerobic Fe0-fed denitrification system (water/wastewater treatment)
ENVO:00002124

Engineered two-member denitrifying consortium of the electroactive bacterium Shewanella oneidensis and the denitrifier Pseudomonas aeruginosa, operated in anaerobic conditions with metallic iron (Fe0) as the sole electron donor. S. oneidensis acts as a bio-engine, using its membrane-bound CymA-OmcA-MtrC complexes to harvest electrons from Fe0 biocorrosion and transfer them to P. aeruginosa. P. aeruginosa accepts these electrons for nitrate reduction, accomplishing microbial denitrification via a Fe0-S. oneidensis-P. aeruginosa electron-transfer system. This Fe0-dependent electro-syntrophy defines a metabolic window for denitrifier growth relevant to nitrate removal in water/wastewater treatment and the global nitrogen cycle.

Taxonomy

Taxon	Ontology ID	Functional Roles	Abundance
Shewanella oneidensis	NCBITaxon:70863	ELECTROGEN ELECTRON_DONOR SYNTROPHIC_PARTNER	N/A
PMID:39504696 - SUPPORT (IN_VITRO) "In a model denitrifying consortium of Shewanella oneidensis and Pseudomonas aeruginosa" PMID:39504696 - SUPPORT (IN_VITRO) "using Fe0 as the sole electron donor, S. oneidensis can act as a bio-engine to harvest electrons and conserve energy from Fe0 biocorrosion"
Pseudomonas aeruginosa	NCBITaxon:287	ELECTROTROPH ELECTRON_ACCEPTOR SYNTROPHIC_PARTNER	N/A
PMID:39504696 - SUPPORT (IN_VITRO) "In a model denitrifying consortium of Shewanella oneidensis and Pseudomonas aeruginosa" PMID:39504696 - SUPPORT (IN_VITRO) "P. aeruginosa can accept electrons for nitrate reduction via the constructed electron transfer system of Fe0-S. oneidensis-P. aeruginosa"

Ecological Interactions

Taxon

Cross-feeding

Mutualism

Syntrophy

Competition

Commensalism

Niche partitioning

Colonization facilitation

Strain competition

Predation

Fe0-dependent electro-syntrophic electron transfer

SYNTROPHY

Source Taxon: Shewanella oneidensis

Target Taxon: Pseudomonas aeruginosa

Evidence

PMID:39504696 - SUPPORT (IN_VITRO)

"Electrons released by S. oneidensis are utilized by P. aeruginosa for accomplishing microbial denitrification."
PMID:39504696 - SUPPORT (IN_VITRO)

"the direct electron cross-feeding process facilitates the expression of genes encoding for denitrification enzymes, intracellular electron transfer proteins, and quorum sensing of P. aeruginosa"

Growth Media

Anaerobic Fe0-fed denitrification medium

Atmosphere ANAEROBIC

Composition

Component	Concentration	Unit	CHEBI
metallic iron (Fe0)	N/A	N/A	N/A
nitrate	N/A	N/A	N/A

Preparation notes: Metallic iron (Fe0) supplied as the sole electron donor; nitrate serves as the terminal electron acceptor for denitrification by P. aeruginosa. No medium composition detail, temperature, pH, or reactor configuration was recoverable — the primary source (Water Research, PMID:39504696) is paywalled and only abstract-level conditions could be extracted.

Evidence

PMID:39504696

"In natural or engineered anaerobic environments"

PMID:39504696

"using Fe0 as the sole electron donor"

PMID:39504696

"P. aeruginosa can accept electrons for nitrate reduction"