A defined starch-fed microbial fuel cell community pairing the exoelectrogen Shewanella oneidensis MR-1 with the starch-fermenting lactic acid producer Streptococcus bovis 148. The system was tested in two configurations: two-step fermentation, where S. bovis first converted starch to lactic acid before S. oneidensis generated electricity, and parallel fermentation, where cultivation and fermentation by both organisms occurred simultaneously. The community is relevant to DOE bioenergy and bioelectrochemical systems because it couples conversion of a polymeric biomass substrate to lactate cross-feeding and extracellular electron transfer in a microbial fuel cell.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Shewanella oneidensis MR-1 | NCBITaxon:211586 |
CROSS_FEEDER
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ABUNDANT |
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| Streptococcus bovis 148 | NCBITaxon:1335 |
PRIMARY_DEGRADER
CROSS_FEEDER
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ABUNDANT |
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Ecological Interactions
Starch Fermentation to Lactic Acid
CROSS_FEEDINGSource Taxon: Streptococcus bovis 148
Target Taxon: Shewanella oneidensis MR-1
Metabolites: starch (CHEBI:28017), lactate (CHEBI:24996)
Biological Processes:
- fermentation (GO:0006113)
Evidence
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PMID:28434977 - SUPPORT (IN_VITRO)"Streptococcus bovis 148 metabolizes starch and produces lactic acid"
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PMID:28434977 - SUPPORT (IN_VITRO)"starch was first converted to lactic acid by S. bovis 148"
Lactate-Fed Current Generation
CROSS_FEEDINGSource Taxon: Shewanella oneidensis MR-1
Target Taxon: Streptococcus bovis 148
Metabolites: lactate (CHEBI:24996)
Biological Processes:
- electron transfer activity (GO:0009055)
Evidence
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PMID:28434977 - SUPPORT (IN_VITRO)"Shewanella oneidensis MR-1 generates electricity from lactic acid"
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PMID:28434977 - SUPPORT (IN_VITRO)"maximum currents at each term were 1.8, 2.4, and 2.8 mA"
Parallel Starch Fermentation and Electrogenesis
CROSS_FEEDINGSource Taxon: Streptococcus bovis 148
Target Taxon: Shewanella oneidensis MR-1
Metabolites: starch (CHEBI:28017), lactate (CHEBI:24996)
Biological Processes:
- fermentation (GO:0006113)
- electron transfer activity (GO:0009055)
Evidence
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PMID:28434977 - SUPPORT (IN_VITRO)"cultivation and subsequent fermentation processes of S. bovis 148 and S. oneidensis MR-1 were performed simultaneously"
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PMID:28434977 - SUPPORT (IN_VITRO)"starch was also converted into lactic acid with electricity generation"
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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Exact-system primary publication - starch-fueled two-organism MFC
Primary publication reporting starch-fueled microbial fuel cells using S. oneidensis MR-1 and S. bovis 148. |
OTHER | PMID:28434977 |
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DOI landing page - Journal of Bioscience and Bioengineering article
DOI landing page for the exact-system starch-fed microbial fuel cell article. |
OTHER | doi:10.1016/j.jbiosc.2017.03.016 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Starch substrate | starch | N/A |
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| Lactic acid intermediate | lactic acid produced from starch | N/A |
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| Parallel fermentation mode | simultaneous cultivation and fermentation | N/A |
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