Engineered two-species electro-syntrophic co-culture of Shewanella oneidensis MR-1 (the electron-donating partner) and Rhodopseudomonas palustris (the electron-accepting partner) built to study and enhance artificial interspecies electron transfer (IET). In-situ polydopamine (PDA) nano-encapsulation of the S. oneidensis MR-1 surface created a conductive, adhesive cell-to-cell interface that promoted stable coaggregation of the two partners and shifted the dominant IET mode from H2-mediated MIET toward contact-dependent direct electron transfer involving outer-membrane c-type cytochromes. Nitrogenase-derived CH4, used as a quantitative indicator of IET efficiency, increased by roughly 380% in the engineered community.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Shewanella oneidensis MR-1 — ontology label: Shewanella oneidensis | NCBITaxon:70863 · TaxonMech · TraitMech |
ELECTRON_DONOR
ELECTROGEN
SYNTROPHIC_PARTNER
|
N/A |
|
|||
| Rhodopseudomonas palustris | NCBITaxon:1076 · TaxonMech · TraitMech |
ELECTRON_ACCEPTOR
ELECTROTROPH
SYNTROPHIC_PARTNER
|
N/A |
|
|||
Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Polydopamine-Driven Cell-to-Cell Coaggregation
The source reports increased coaggregation of Shewanella oneidensis MR-1 and Rhodopseudomonas palustris with the material-associated cell interface. This is a two-member physical community observation, not itself a donor-to-recipient metabolic transfer or evidence of syntrophic feedback.
Participating Taxa: Shewanella oneidensis MR-1, Rhodopseudomonas palustris
Biological Processes:
- cell aggregation (GO:0098743 · PathwayMech)
PARTIAL: the material-associated interface supports contact between the two members and is reported to favor contact-dependent electron transfer. The abstract does not isolate coaggregation from the interface's conductive properties as the causal mediator of the transfer-mode shift.
Evidence
-
PMID:42285537 - SUPPORT (IN_VITRO)"The coating promoted stable and intimate coaggregation of SW with Rhodopseudomonas palustris (RP, electron accepting partner)."
Shift Toward Contact-Dependent Electron Transfer
The primary abstract reports a shift from H2-mediated transfer toward contact-dependent electron transfer in the tested two-species community, with Shewanella as donor and Rhodopseudomonas as acceptor. Retain that direction without treating a shift in the dominant mode as elimination of all mediated transfer or as evidence of product-removal feedback.
Source Taxon: Shewanella oneidensis MR-1
Target Taxon: Rhodopseudomonas palustris
Metabolites: hydrogen (H2) (CHEBI:18276 · MediaIngredientMech · CMMMech)
Biological Processes:
- electron transport chain (GO:0022900 · PathwayMech)
PARTIAL: the reported transfer-mode shift is proposed to support the increased nitrogenase-derived methane readout. The abstract presents methane as an IET indicator, but does not establish a calibrated proportional increase in all interspecies electron flux or separate the contribution of each transfer route.
Evidence
-
PMID:42285537 - SUPPORT (IN_VITRO)"This cell-to-cell PDA interface promoted a shift in the dominant IET mode from H2-mediated MIET toward contact-dependent electron transfer involving outer membrane C-type cytochromes."
Enhanced Nitrogenase-Derived CH4 Indicator
The engineered two-species community showed roughly 380% higher nitrogenase-derived methane, used by the authors as an indicator of interspecies electron-transfer efficiency. Preserve this measured positive result without equating the percentage with a calibrated change in total electron flux, natural methanogenesis or reciprocal fitness benefit.
Participating Taxa: Shewanella oneidensis MR-1, Rhodopseudomonas palustris
Metabolites: methane (CHEBI:16183 · MediaIngredientMech · CMMMech)
Evidence
-
PMID:42285537 - SUPPORT (IN_VITRO)"nitrogenase-derived CH4, used as a quantitative indicator of IET efficiency, increased by ∼380 % in the engineered two-species community."
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN How do interface effects and indicator calibration constrain causal attribution?
The fresh primary abstract and whole committed cache support coaggregation, the reported transfer-mode shift and the methane-indicator increase. Complete main text, route-specific controls and indicator calibration were not independently reviewed. The retained PARTIAL directions distinguish interface-associated effects from isolated mediation and a quantitative proxy from total electron flux; no product-removal feedback or reciprocal fitness benefit is newly asserted.
Attaches to: ecological_interactions#Polydopamine-Driven Cell-to-Cell Coaggregation, ecological_interactions#Shift Toward Contact-Dependent Electron Transfer, ecological_interactions#Enhanced Nitrogenase-Derived CH4 Indicator
1 evidence item(s)
nitrogenase-derived CH4, used as a quantitative indicator of IET efficiency, increased by ∼380 % in the engineered two-species community.
Supports the reported indicator increase, not a separately calibrated total electron-flux increase or syntrophic feedback.
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