Sorghum SRC1 Subset Community

Community ID CommunityMech:000063

Ecological State ENGINEERED

Environment rhizosphere
ENVO:00005801 · HabitatMech ↗

A simplified synthetic rhizosphere community (SRC1-subset) derived from the larger SRC1 consortium for enhancing sorghum bioenergy crop productivity. The subset consists of 16-20 bacterial strains selected from the full 57-strain SRC1 community, including multiple Pseudomonas, Bacillus, and Arthrobacter strains. This engineered community was designed using network analysis to identify hub taxa and strains capable of utilizing sorghum-specific root exudate compounds. Field-tested studies demonstrate that the community effectively colonizes sorghum rhizosphere and roots, leading to increased shoot biomass and improved yield performance under field conditions. The growth enhancement correlates with transcriptional dampening of lignin biosynthesis in the host plant.

Taxonomy

Taxon	Ontology ID	Abundance
Pseudomonas sp.	NCBITaxon:306 · TaxonMech · TraitMech	ABUNDANT
doi:10.1093/ismejo/wrae126 - SUPPORT (IN_VIVO) "Here, we engineered a synthetic rhizosphere community (SRC1) with the anticipation that it would exhibit a selective advantage in colonizing the host Sorghum bicolor, thereby potentially fostering its growth"
Bacillus sp. — ontology label: Bacillus <firmicutes>	NCBITaxon:1386 · TaxonMech · TraitMech	ABUNDANT
doi:10.1093/ismejo/wrae126 - SUPPORT (IN_VIVO) "Despite the acknowledged potential for enhancing plant fitness by manipulating the rhizosphere, the engineering of the rhizosphere microbiome through inoculation has posed significant challenges"
Arthrobacter sp.	NCBITaxon:1667 · TaxonMech · TraitMech	ABUNDANT
doi:10.1093/ismejo/wrae126 - SUPPORT (IN_VIVO) "Here, we engineered a synthetic rhizosphere community (SRC1) with the anticipation that it would exhibit a selective advantage in colonizing the host Sorghum bicolor, thereby potentially fostering its growth"
Streptomyces ambofaciens	NCBITaxon:1889 · TaxonMech · TraitMech	COMMON
doi:10.1093/ismejo/wrae126 - SUPPORT (IN_VIVO) "SRC1 was assembled from bacterial isolates identified either for their potential role in community cohesion through network analysis or for their ability to benefit from host-specific exudate compounds"
Paenibacillus lautus	NCBITaxon:1401 · TaxonMech · TraitMech	COMMON
doi:10.1093/ismejo/wrae126 - SUPPORT (IN_VIVO) "SRC1 was assembled from bacterial isolates identified either for their potential role in community cohesion through network analysis or for their ability to benefit from host-specific exudate compounds"
Pseudomonas pudica	NCBITaxon:272772 · TaxonMech · TraitMech	COMMON
doi:10.1093/ismejo/wrae126 - SUPPORT (IN_VIVO) "SRC1 was assembled from bacterial isolates identified either for their potential role in community cohesion through network analysis or for their ability to benefit from host-specific exudate compounds"

Ecological Interactions

ℹ️ No detailed ecological interaction data is available for this community.

This record may be a metabolic model or minimal entry containing only taxonomy and external resource links.

Knowledge gaps & discussions (1)

KNOWLEDGE_GAP OPEN Which exact source-backed community does the claimed 16-20-member SRC1 subset represent?

The record claims a subset but cites parent SRC1 results. The primary paper introduction identifies 57 strains, independently corroborated by the SRC2 primary study; the cached and freshly retrieved SRC1 abstract does not identify a 16-20-member formulation. Four subset-attributed nodes are withheld, not a rejection of the parent study's positive observations. Cohesion does not establish facilitation, host benefit does not establish reciprocal mutualism, and lignin transcription is not proven mediation. Resolve identity, membership and endpoint scope in issue #1813 before restoring a graph. Top-level identity/taxonomy are preserved pending that separately scoped decision; this record remains needs_research.

1 evidence item(s)

IN_VIVO NO_EVIDENCE doi:10.1093/ismejo/wrae126

Here, we engineered a synthetic rhizosphere community (SRC1) with the anticipation that it would exhibit a selective advantage in colonizing the host Sorghum bicolor, thereby potentially fostering its growth

Identifies the studied SRC1 broadly, but does not identify the record-specific subset.

Environmental Factors

Factor	Value	Unit
Community design strategy	network-based and exudate-responsive selection	N/A
Community size	16-20 member subset from 57-strain parent consortium	N/A
Field testing	validated in native field conditions	N/A
Biomass enhancement	increased shoot fresh and dry weight	N/A
Bioenergy crop application	sorghum productivity improvement	N/A
Inoculation method	1 mg/mL biomass per strain	N/A

Growth Media

unspecified solid media (in vitro); gnotobiotic in planta

Preparation notes: SRC1 growth was assessed in vitro on solid media, in planta under gnotobiotic laboratory conditions, and in the field; community cohesion was most robust when cultivated with the Sorghum bicolor host under laboratory conditions. No medium composition, temperature, or pH is reported in the accessible abstract.

Evidence

doi:10.1093/ismejo/wrae126

"The growth performance of SRC1 was assessed in vitro on solid media, in planta under gnotobiotic laboratory conditions, and in the field"

Cross-Mech Suite Links

Explore related entities across the CultureBotAI X-Mech knowledge bases:

HabitatMech: rhizosphere ↗ TaxonMech: Pseudomonas sp. ↗ TraitMech: Pseudomonas sp. ↗ TaxonMech: Bacillus sp. ↗ TraitMech: Bacillus sp. ↗ TaxonMech: Arthrobacter sp. ↗ TraitMech: Arthrobacter sp. ↗ TaxonMech: Streptomyces ambofaciens ↗ TraitMech: Streptomyces ambofaciens ↗ TaxonMech: Paenibacillus lautus ↗ TraitMech: Paenibacillus lautus ↗ TaxonMech: Pseudomonas pudica ↗ TraitMech: Pseudomonas pudica ↗ CultureMech Growth Media ↗ PathwayMech Pathways ↗