Natural thaw-gradient peat microbial communities from Stordalen Mire in Arctic Sweden, curated around genome-resolved multi-omics evidence for direct and indirect methylotrophic routes to methane production. The study expanded genomic representation of native methanogens, identified methanogenic MAGs with methylotrophic potential and transcriptional activity, detected broad bacterial anaerobic methylotrophic potential, and observed active methylotrophy across palsa, bog, and fen stages of permafrost thaw. The community is DOE-relevant through USDOE Office of Science BER, PNNL EMSL, and JGI involvement and links permafrost thaw, wetland carbon cycling, and methane emissions.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Stordalen Mire thaw-gradient microbiome | NCBITaxon:131567 |
PRIMARY_DEGRADER
SECONDARY_FERMENTER
SYNTROPHIC_PARTNER
|
N/A |
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| Stordalen Mire methanogenic archaea | NCBITaxon:2157 |
SYNTROPHIC_PARTNER
|
COMMON |
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| Stordalen Mire anaerobic methylotrophic bacteria | NCBITaxon:2 |
PRIMARY_DEGRADER
CROSS_FEEDER
|
N/A |
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Ecological Interactions
Methanogenic Methylotrophy Across Thaw Gradient
SYNTROPHYSource Taxon: Stordalen Mire methanogenic archaea
Metabolites: methane (CHEBI:16183), methanol (CHEBI:17790)
Biological Processes:
- methanogenesis (GO:0015948)
Evidence
-
PMID:38063415 - SUPPORT (COMPUTATIONAL)"Methanogenesis across increasing permafrost thaw is thus revised"
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PMID:38063415 - SUPPORT (COMPUTATIONAL)"co-occurrence of methylotrophy throughout"
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PMID:30013118 - PARTIAL (COMPUTATIONAL)"Microbial and geochemical data highlight lineages that correlate with the production of greenhouse gases and indicate novel syntrophic relationships"
Bacterial Anaerobic Methylotrophy
CROSS_FEEDINGSource Taxon: Stordalen Mire anaerobic methylotrophic bacteria
Metabolites: methanol (CHEBI:17790)
Biological Processes:
- organic substance catabolic process (GO:0071704)
Evidence
-
PMID:38063415 - SUPPORT (COMPUTATIONAL)"In addition to methanogenic methylotrophy, >1,700 bacterial MAGs"
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PMID:38063415 - SUPPORT (COMPUTATIONAL)"bacterial methylotrophy may be an important and previously underappreciated component"
Methylated-Compound Substrate Niche Partitioning
NICHE_PARTITIONINGMetabolites: methanol (CHEBI:17790)
Evidence
-
PMID:38063415 - SUPPORT (COMPUTATIONAL)"Metabolomic analyses revealed the presence of diverse methylated compounds in the Mire"
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PMID:38063415 - SUPPORT (COMPUTATIONAL)"Methanosarcinales and Methanobacteriales MAGs, these data indicated the use of methylated oxygen compounds"
Thaw-Stage Activity Gradient
NICHE_PARTITIONINGEvidence
-
PMID:38063415 - SUPPORT (IN_VIVO)"the most frozen non-methanogenic palsa found to host bacterial methylotrophy"
-
PMID:38063415 - SUPPORT (IN_VIVO)"partially thawed bog and fully thawed fen seen to house both methanogenic and bacterial methylotrophic activities"
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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Primary publication for Stordalen Mire methylotrophic methanogenesis
PubMed record for the mSystems paper reporting genome-resolved multi-omics of methylotrophic methanogenesis and bacterial methylotrophy in Stordalen Mire. |
OTHER | PMID:38063415 |
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DOI landing page
DOI for the mSystems publication on methylotrophic methane production in Stordalen Mire. |
OTHER | doi:10.1128/msystems.00698-23 |
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DOE OSTI record
DOE PAGES/OSTI record listing PNNL EMSL, USDOE JGI, and BER support for the Stordalen Mire methylotrophy study. |
OTHER | OSTI:2377155 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Permafrost thaw stage | palsa, bog, and fen thaw-gradient habitats | N/A |
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| Methylated compounds | diverse methylated compounds, including known methylotrophic substrates | N/A |
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| Methane emissions | wetland methane emissions under climate change | N/A |
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