A sequential synthetic microbial community (SynCom)-microalga coupling system engineered to treat livestock biogas slurry. An autochthonous microalga, Chlorella sorokiniana, isolated from the slurry is coupled with a SynCom pretreatment stage. The system operated stably for 60 days, achieving 94.41% nitrate-nitrogen removal, 81.84% total-phosphorus removal, and 6.31% chemical oxygen demand removal. SynCom pretreatment increased the biomass and soluble protein content of C. sorokiniana (by 27.15% and 488.10% vs the BG11 control), upregulating Rubisco and glutamine synthetase, providing a strategy for simultaneous pollutant abatement and resource recovery.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Chlorella sorokiniana | NCBITaxon:3076 · TaxonMech · TraitMech |
PRIMARY_PRODUCER
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N/A |
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| SynCom bacterial members (unnamed) — ontology label: Bacteria | NCBITaxon:2 · TaxonMech · TraitMech | N/A | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Nitrate-Nitrogen and Phosphorus Removal
The sequential SynCom-microalga system operated stably for 60 days and removed 94.41% of nitrate nitrogen, 81.84% of total phosphorus and 6.31% of chemical oxygen demand. These are system-level removal endpoints, not direct evidence of metabolite transfer between the bacterial pretreatment community and the alga or of which organisms carried each removal flux.
Metabolites: nitrate (CHEBI:17632 · MediaIngredientMech · CMMMech)
Evidence
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PMID:41794300 - SUPPORT (IN_VITRO)"94.41% removal of nitrate nitrogen, 81.84% removal of total phosphorus, and 6.31% removal of chemical oxygen demand "
Chlorella biomass and protein response to SynCom pretreatment
C. sorokiniana grown with SynCom-pretreated slurry had 27.15% greater biomass and 488.10% greater soluble protein content than the BG11 comparison. This positive algal response does not isolate bacterial conditioning from all differences between the media, identify an exchanged metabolite, or establish that bacterial fitness was unaffected.
Participating Taxa: Chlorella sorokiniana
Evidence
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PMID:41794300 - SUPPORT (IN_VITRO)"SynCom pretreatment increased the biomass and soluble protein content of C. sorokiniana by 27.15% and 488.10%, respectively"
Chlorella carbon- and nitrogen-assimilation transcript response
Transcriptomic analysis reported upregulated Rubisco and glutamine synthetase genes in C. sorokiniana. This supports a carbon- and nitrogen-assimilation transcriptional response accompanying the biomass and protein phenotype. It does not measure enzyme activity or carbon/nitrogen flux and does not establish that increased growth caused the transcript changes.
Participating Taxa: Chlorella sorokiniana
Biological Processes:
- photosynthesis (GO:0015979 · PathwayMech)
Evidence
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PMID:41794300 - SUPPORT (IN_VITRO)"Transcriptomic analysis showed that the expression levels of key genes Rubisco and glutamine synthetase were upregulated "
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which pretreatment changes mediate the algal growth and transcriptional responses in the sequential slurry system?
The positive 60-day removal and BG11-referenced algal outcomes remain. Construction, duration and coincident expression are not separate causal drivers. The reviewed abstract does not resolve exchanged metabolites, reciprocal fitness, organism-specific removal flux or expression-to-growth mediation. The unnamed bacterial membership remains the separate existing issue183 gap.
Attaches to: ecological_interactions#Nitrate-Nitrogen and Phosphorus Removal, ecological_interactions#Chlorella biomass and protein response to SynCom pretreatment, ecological_interactions#Chlorella carbon- and nitrogen-assimilation transcript response
2 evidence item(s)
SynCom pretreatment increased the biomass and soluble protein content of C. sorokiniana by 27.15% and 488.10%, respectively
Supports the algal biomass/protein response; the abstract names BG11 as the comparator, not an otherwise identical untreated-slurry control.
Transcriptomic analysis showed that the expression levels of key genes Rubisco and glutamine synthetase were upregulated
Supports the carbon- and nitrogen-assimilation transcriptional response in the coupled system.
Growth Media
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