SynCom MetG2 Rhizobacteria Sugarcane Stress Resilience

Community ID CommunityMech:000302

Ecological State ENGINEERED

Environment sugarcane rhizosphere
ENVO:00005801

A rhizobacteria synthetic community (SynCom) named MetG2 inoculated onto sugarcane in a greenhouse experiment to enhance stress resilience through targeted modulation of plant-microbe interactions. Shotgun metagenome and metatranscriptome sequencing revealed temporal shifts in the rhizosphere microbial community, with up-regulation of genes for flagellar assembly, chemotaxis, quorum sensing, and biofilm formation (particularly within Proteobacteria), indicating active microbial movement toward root surfaces, alongside enrichment of genes for nutrient acquisition, hormone synthesis, siderophore production, and VOC synthesis. Sugarcane responded with up-regulation of hormone-related and nutrient-transporter genes and induction of stress-resistance genes, supporting MetG2 as a sustainable strategy to enhance crop productivity and resilience.

Taxonomy

Taxon	Ontology ID	Functional Roles	Abundance
sugarcane (Saccharum officinarum)	NCBITaxon:4547	PRIMARY_PRODUCER	N/A
doi:10.1016/j.rhisph.2025.101142 - SUPPORT (IN_VIVO) "This study investigated the molecular mechanisms underlying sugarcane responses and the dynamics of its rhizosphere microbial community following inoculation with a SynCom called MetG2"
Proteobacteria (rhizosphere community members)	NCBITaxon:1224		N/A
doi:10.1016/j.rhisph.2025.101142 - SUPPORT (IN_VIVO) "particularly within Proteobacteria, indicating active microbial movement toward root surfaces"

Ecological Interactions

Taxon

Cross-feeding

Mutualism

Syntrophy

Competition

Commensalism

Niche partitioning

Colonization facilitation

Strain competition

Predation

Microbial colonization of sugarcane roots

COLONIZATION_FACILITATION

Source Taxon: Proteobacteria (rhizosphere community members)

Target Taxon: sugarcane (Saccharum officinarum)

Evidence

doi:10.1016/j.rhisph.2025.101142 - SUPPORT (IN_VIVO)

"an up-regulation of genes related to flagellar assembly, chemotaxis, quorum sensing, and biofilm formation, particularly within Proteobacteria, indicating active microbial movement toward root surfaces"

SynCom-stimulated enhancement of sugarcane stress resilience

MUTUALISM

Source Taxon: Proteobacteria (rhizosphere community members)

Target Taxon: sugarcane (Saccharum officinarum)

Evidence

doi:10.1016/j.rhisph.2025.101142 - SUPPORT (IN_VIVO)

"genes associated with nutrient acquisition, hormone synthesis, siderophore production, and volatile organic compound (VOC) synthesis were enriched, suggesting stimulated beneficial microbial activities"
doi:10.1016/j.rhisph.2025.101142 - SUPPORT (IN_VIVO)

"Sugarcane plants also exhibited dynamic responses, including the up-regulation of hormone-related genes (auxin, cytokinin, salicylic acid) and nutrient transporter genes"