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SynCom Y Agrobacterium-Bacillus Biofilm Biocontrol Co-culture

Community ID CommunityMech:000272
Ecological State ENGINEERED
Environment rhizosphere
ENVO:00005801

Two-species synthetic bacterial community (SynCom Y) of Agrobacterium deltaense LSQ16 and Bacillus velezensis WB, simplified from a larger biocontrol SynCom Q by stepwise strain omission. Co-cultivation synergistically enhances multispecies biofilm formation, extracellular polysaccharide production, and metabolic activity, improving suppression of watermelon Fusarium wilt. Genome-scale modeling predicts interspecies metabolite exchange (amino acids, purines, phosphates); exogenous L-ornithine and guanine enhance B. velezensis biofilm formation, consistent with cross-feeding-supported cooperation.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Agrobacterium deltaense LSQ16 NCBITaxon:1183412
CROSS_FEEDER
N/A
  • PMID:42125264 - SUPPORT (IN_VITRO)
    "Co-cultivation of A. deltaense LSQ16 and B. velezensis WB significantly enhanced biofilm formation."
Bacillus velezensis WB NCBITaxon:492670
CROSS_FEEDER
N/A
  • PMID:42125264 - SUPPORT (IN_VITRO)
    "Co-cultivation of A. deltaense LSQ16 and B. velezensis WB significantly enhanced biofilm formation."

Ecological Interactions

Ecological interaction network for SynCom Y Agrobacterium-Bacillus Biofilm Biocontrol Co-culture Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Synergistic biofilm and EPS formation in co-culture

MUTUALISM

Source Taxon: Agrobacterium deltaense LSQ16

Target Taxon: Bacillus velezensis WB

Evidence

  • PMID:42125264 - SUPPORT (IN_VITRO)
    "Compared with monocultures, co-cultivation also markedly increased extracellular polysaccharide production and metabolic activity."

Predicted cross-feeding from A. deltaense LSQ16 to B. velezensis WB

CROSS_FEEDING

Source Taxon: Agrobacterium deltaense LSQ16

Target Taxon: Bacillus velezensis WB

Metabolites: L-ornithine (CHEBI:15729), guanine (CHEBI:16235), guanosine (CHEBI:16750), (S)-malate (CHEBI:15589), phosphate (CHEBI:26020)

Evidence

  • PMID:42125264 - SUPPORT (IN_VITRO)
    "Moreover, exogenous supplementation of l-ornithine and guanine further enhanced the biofilm-forming capacity of B. velezensis WB."
  • PMID:42125264 - SUPPORT (COMPUTATIONAL)
    "Predictive results indicated metabolite exchange between the two strains involved amino acids, purines, phosphates, and other compounds."

Predicted metabolite return from B. velezensis WB to A. deltaense LSQ16

CROSS_FEEDING

Source Taxon: Bacillus velezensis WB

Target Taxon: Agrobacterium deltaense LSQ16

Metabolites: L-serine (CHEBI:17115)

Evidence

  • PMID:42125264 - SUPPORT (COMPUTATIONAL)
    "Predictive results indicated metabolite exchange between the two strains involved amino acids, purines, phosphates, and other compounds."

Growth Media

LBGM

Temperature 30 °C

Preparation notes: Biofilm formation assay. Strains precultured in TSB overnight at 25 °C with shaking at 150 rpm, OD600 adjusted to 0.8, then inoculated at 10% (v/v) into 2 mL LBGM medium per well. Single-species suspensions added directly to 24-well plates; mixed-species biofilms mixed in equal volumes before plating. Incubated statically at 30 °C and sampled at 24, 48, and 72 h.

Evidence

PMID:42125264

"Subsequently, they were inoculated at 10% ( v / v ) into 2 mL of LBGM medium. For single-species biofilms, the bacterial suspension was directly introduced into 24-well plates."

PMID:42125264

"Samples were incubated statically at 30 °C for 24, 48, and 72 h to compare the biofilm-forming capacities"

PMID:42125264

"Glycerol-preserved strains were inoculated into TSB and cultured overnight at 25 °C with shaking at 150 rpm."