← Back to Communities

Synthetic Periphyton Freshwater Biofilm

Community ID CommunityMech:000130
Ecological State ENGINEERED
Environment freshwater periphyton biofilm
ENVO:01000306

A 26-member phototrophic synthetic periphyton biofilm used as a reproducible freshwater model system for species dynamics and stressor response.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
diatoms NCBITaxon:2836
PRIMARY_PRODUCER
N/A
  • PMID:35869094 - SUPPORT (IN_VITRO)
    "we rationally developed a workflow to construct a synthetic community by co-culturing 26 phototrophic species (i.e., diatoms, green algae, and cyanobacteria)"
green algae NCBITaxon:3041
PRIMARY_PRODUCER
N/A
  • PMID:35869094 - SUPPORT (IN_VITRO)
    "we rationally developed a workflow to construct a synthetic community by co-culturing 26 phototrophic species (i.e., diatoms, green algae, and cyanobacteria)"
cyanobacteria NCBITaxon:1117
PRIMARY_PRODUCER
N/A
  • PMID:35869094 - SUPPORT (IN_VITRO)
    "we rationally developed a workflow to construct a synthetic community by co-culturing 26 phototrophic species (i.e., diatoms, green algae, and cyanobacteria)"

Ecological Interactions

Ecological interaction network for Synthetic Periphyton Freshwater Biofilm Bipartite graph where circle nodes represent taxa and each ecological interaction is drawn as a distinct non-circular symbol, with colour repeating the same distinction (colonization facilitation).
Taxon
Colonization facilitation

Successional Periphyton Biofilm Assembly

COLONIZATION_FACILITATION

Evidence

  • PMID:35869094 - SUPPORT (IN_VITRO)
    "we rationally developed a workflow to construct a synthetic community by co-culturing 26 phototrophic species (i.e., diatoms, green algae, and cyanobacteria)"

Environmental Factors

Factor Value Unit
successional inoculation on glass slides Phototrophic species were inoculated sequentially to construct a synthetic periphytic biofilm. N/A
  • PMID:35869094 - SUPPORT (IN_VITRO)
    "we rationally developed a workflow to construct a synthetic community by co-culturing 26 phototrophic species (i.e., diatoms, green algae, and cyanobacteria)"

Growth Media

COMBO freshwater medium

Temperature 17 °C

Preparation notes: 26 phototrophic species pre-cultured in COMBO and inoculated in a successional sequence onto glass slides to build the biofilm. Warming (17 to 20 °C) and the herbicide terbuthylazine (0-100 nM) were applied as stressors.

Evidence

PMID:35869094

"to grow in the same freshwater medium COMBO (see Supplementary Table 2 for the detailed composition) 77 at 17 °C and photon irradiance of 40 to 60 µmol s −1"

PMID:35869094

"with a photoperiod of 16 h light: 8 h dark"

PMID:35869094

"sterile 300-mL Erlenmeyer flasks containing 150 mL of COMBO and placed on a shaker operated at 30 rpm"