A synthetic filamentous coculture pairing the cellulolytic fungus Trichoderma reesei RUT-C30 with the noncellulolytic bacterium Streptomyces coelicolor A3(2). The coculture was designed to use cellulose as the carbon source, making S. coelicolor dependent on hydrolysate sugars released by T. reesei cellulases. The system is DOE-relevant as a controllable model for lignocellulose-oriented coculture bioprocessing, where population dynamics, cellulase formation, and secondary-metabolite/pigment production can be tuned through inoculation ratio and abiotic process parameters.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Trichoderma reesei RUT-C30 | NCBITaxon:1344414 |
PRIMARY_DEGRADER
CROSS_FEEDER
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ABUNDANT |
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| Streptomyces coelicolor A3(2) | NCBITaxon:100226 |
CROSS_FEEDER
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ABUNDANT |
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Ecological Interactions
Cellulase-Driven Hydrolysate Cross-Feeding
CROSS_FEEDINGSource Taxon: Trichoderma reesei RUT-C30
Target Taxon: Streptomyces coelicolor A3(2)
Metabolites: cellulose (CHEBI:18246)
Biological Processes:
- cellulose catabolic process (GO:0030245)
- carbohydrate metabolic process (GO:0005975)
Evidence
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PMID:36314761 - SUPPORT (IN_VITRO)"cellulose as a carbon source"
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PMID:36314761 - SUPPORT (IN_VITRO)"hydrolysate sugars released by T. reesei cellulases"
Noncellulolytic Partner Dependency
COMMENSALISMSource Taxon: Trichoderma reesei RUT-C30
Target Taxon: Streptomyces coelicolor A3(2)
Metabolites: cellulose (CHEBI:18246)
Biological Processes:
- carbohydrate metabolic process (GO:0005975)
Evidence
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PMID:36314761 - SUPPORT (IN_VITRO)"dependency of S. coelicolor on hydrolysate sugars"
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PMID:36314761 - SUPPORT (IN_VITRO)"noncellulolytic bacterium Streptomyces coelicolor A3(2)"
Tunable Filamentous Population Dynamics
COMPETITIONBiological Processes:
- secondary metabolite biosynthetic process (GO:0044550)
Evidence
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PMID:36314761 - SUPPORT (IN_VITRO)"both partners outcompete the other when given a high inoculation advantage"
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PMID:36314761 - SUPPORT (IN_VITRO)"simultaneous growth of both partners, cellulase, and pigment production could be determined"
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PMID:36314761 - SUPPORT (IN_VITRO)"population dynamics were also tuned by modulating abiotic factors"
Associated Datasets
| Dataset | Type | Repository | Accession |
|---|---|---|---|
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Trichoderma-Streptomyces filamentous coculture publication
PubMed and PMC-indexed MicrobiologyOpen study reporting respiration, fluorescence, inoculation-ratio, osmolality, shaking-frequency, cellulase, and pigment-production phenotypes for the T. reesei RUT-C30 and S. coelicolor A3(2) coculture. |
PHENOTYPE | OTHER | PMID:36314761 |
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Cellulose carbon source | cellulose | N/A |
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| Inoculation ratio | varied | N/A |
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| Osmolality | increased osmolality favored S. coelicolor | N/A |
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| Shaking frequency | increased shaking frequency reduced S. coelicolor biomass formation | N/A |
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