A systematic framework for assembling rhizosphere-derived Reduced Complexity Consortia (RCC) from Brachypodium distachyon roots grown in field soil from Angelo Coast Range Reserve, California. Enriched co-localized microbes with carbon substrates mimicking root exudates, generating 768 enrichments. Transferred every 3 or 7 days for 10 generations to develop fast and slow-growing reduced complexity communities. Keystone taxa identified within these networks belong to genera with plant growth-promoting traits. Tested consortia demonstrated high stability and reproducibility, assuring successful revival from glycerol stocks.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Methylobacterium | NCBITaxon:407 |
CROSS_FEEDER
|
N/A |
|
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| Stenotrophomonas | NCBITaxon:40323 |
PRIMARY_DEGRADER
|
N/A |
|
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| Pseudomonas | NCBITaxon:286 |
PRIMARY_DEGRADER
|
N/A |
|
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| Terriglobus | NCBITaxon:442709 |
PRIMARY_DEGRADER
|
N/A |
|
|||
| Arthrobacter | NCBITaxon:1667 |
PRIMARY_DEGRADER
|
N/A |
|
|||
| Burkholderia | NCBITaxon:32008 |
PRIMARY_DEGRADER
|
N/A |
|
|||
| Paenibacillus | NCBITaxon:44249 |
PRIMARY_DEGRADER
|
N/A |
|
|||
| Sphingomonas | NCBITaxon:13687 |
CROSS_FEEDER
|
N/A |
|
|||
| Mucilaginibacter | NCBITaxon:423349 |
CROSS_FEEDER
|
N/A |
|
|||
| Bradyrhizobium | NCBITaxon:374 |
CROSS_FEEDER
|
N/A |
|
|||
Ecological Interactions
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation
Carbon Substrate-Driven Community Differentiation
COMPETITIONEvidence
-
PMID:38846575 - SUPPORT (IN_VITRO)"complex carbon enriching slow growing yet functionally important soil taxa like Acidobacteria and Verrucomicrobia"
Fast vs Slow Growth Rate Selection
COMPETITIONEvidence
-
PMID:38846575 - SUPPORT (IN_VITRO)"complex carbon enriching slow growing yet functionally important soil taxa like Acidobacteria and Verrucomicrobia"
Associated Datasets
| Dataset | Type | Repository | Accession |
|---|---|---|---|
|
16S rRNA Amplicon Sequencing
16S rRNA amplicon sequencing data from enrichment consortia across carbon substrates and transfer frequencies. |
AMPLICON_16S | NCBI_BIOPROJECT | PRJNA990818 |
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Carbon Substrate | glucose, glucuronic acid, glutamine, asparagine, 1/10 R2A | N/A |
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| Transfer Frequency | 3-day (fast) or 7-day (slow) | N/A |
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| Soil Source | Angelo Coast Range Reserve, California | N/A |