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m-CAFEs Brachypodium Reduced Complexity Consortia

Community ID CommunityMech:000078
Ecological State ENGINEERED
Environment Brachypodium distachyon rhizosphere
ENVO:00005801

A systematic framework for assembling rhizosphere-derived Reduced Complexity Consortia (RCC) from Brachypodium distachyon roots grown in field soil from Angelo Coast Range Reserve, California. Enriched co-localized microbes with carbon substrates mimicking root exudates, generating 768 enrichments. Transferred every 3 or 7 days for 10 generations to develop fast and slow-growing reduced complexity communities. Keystone taxa identified within these networks belong to genera with plant growth-promoting traits. Tested consortia demonstrated high stability and reproducibility, assuring successful revival from glycerol stocks.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Methylobacterium NCBITaxon:407
CROSS_FEEDER
N/A
Stenotrophomonas NCBITaxon:40323
PRIMARY_DEGRADER
N/A
Pseudomonas NCBITaxon:286
PRIMARY_DEGRADER
N/A
Terriglobus NCBITaxon:442709
PRIMARY_DEGRADER
N/A
Arthrobacter NCBITaxon:1667
PRIMARY_DEGRADER
N/A
Burkholderia NCBITaxon:32008
PRIMARY_DEGRADER
N/A
Paenibacillus NCBITaxon:44249
PRIMARY_DEGRADER
N/A
Sphingomonas NCBITaxon:13687
CROSS_FEEDER
N/A
Mucilaginibacter NCBITaxon:423349
CROSS_FEEDER
N/A
Bradyrhizobium NCBITaxon:374
CROSS_FEEDER
N/A

Ecological Interactions

Ecological interaction network for m-CAFEs Brachypodium Reduced Complexity Consortia Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism

Carbon Substrate-Driven Community Differentiation

COMPETITION

Evidence

  • PMID:38846575 - SUPPORT (IN_VITRO)
    "complex carbon enriching slow growing yet functionally important soil taxa like Acidobacteria and Verrucomicrobia"

Fast vs Slow Growth Rate Selection

COMPETITION

Evidence

  • PMID:38846575 - SUPPORT (IN_VITRO)
    "complex carbon enriching slow growing yet functionally important soil taxa like Acidobacteria and Verrucomicrobia"

Associated Datasets

Dataset Type Repository Accession
16S rRNA Amplicon Sequencing
16S rRNA amplicon sequencing data from enrichment consortia across carbon substrates and transfer frequencies. 		AMPLICON_16S NCBI_BIOPROJECT PRJNA990818

Environmental Factors

Factor Value Unit
Carbon Substrate glucose, glucuronic acid, glutamine, asparagine, 1/10 R2A N/A
Transfer Frequency 3-day (fast) or 7-day (slow) N/A
Soil Source Angelo Coast Range Reserve, California N/A