Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_EG_JM.pdf
Composition
flowchart LR
CultureMech_000071["eg_jm"]
ing0_Sodium_acetate_trihydrate["Sodium acetate trihydrate"]
CultureMech_000071 --> ing0_Sodium_acetate_trihydrate
CHEBI_32138["CHEBI:32138"]
ing0_Sodium_acetate_trihydrate -.-> CHEBI_32138
ing1_Lab_Lemco_powder["Lab-Lemco powder"]
CultureMech_000071 --> ing1_Lab_Lemco_powder
ing2_Tryptone["Tryptone"]
CultureMech_000071 --> ing2_Tryptone
ing3_Yeast_extract["Yeast extract"]
CultureMech_000071 --> ing3_Yeast_extract
ing4_Add_the_above_constituents_and_make_up_to_1_litre_with_deionised_water__For_agar__add["Add the above constituents and make up to 1 litre with deionised wa..."]
CultureMech_000071 --> ing4_Add_the_above_constituents_and_make_up_to_1_litre_with_deionised_water__For_agar__add
ing5_Na_EDTA["Na EDTA"]
CultureMech_000071 --> ing5_Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing5_Na_EDTA -.-> CHEBI_64734
ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000071 --> ing6_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
ing7_Thiamine_HCl["Thiamine HCl"]
CultureMech_000071 --> ing7_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing7_Thiamine_HCl -.-> CHEBI_49105
ing8_Biotin["Biotin"]
CultureMech_000071 --> ing8_Biotin
CHEBI_15956["CHEBI:15956"]
ing8_Biotin -.-> CHEBI_15956
ing9_Make_up_to_1_litre_with_deionised_water__For_agar__add["Make up to 1 litre with deionised water. For agar, add"]
CultureMech_000071 --> ing9_Make_up_to_1_litre_with_deionised_water__For_agar__add
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Sodium acetate trihydrate
C2H3O2.3H2O.Na 136.079 g/mol
|
1.0 G_PER_L |
CHEBI:32138
sodium acetate trihydrate
|
Ontology mapping not yet available; From CCAP Medium MR_EG_JM.pdf; Curated from CCAP Medium MR_EG_JM.pdf specification |
| 2 | Lab-Lemco powder | 1.0 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_EG_JM.pdf; Curated from CCAP Medium MR_EG_JM.pdf specification | |
| 3 | Tryptone | 2.0 G_PER_L |
MICRO:0000182 (source link unavailable)
Tryptone
|
From CCAP Medium MR_EG_JM.pdf; Curated from CCAP Medium MR_EG_JM.pdf specification |
| 4 | Yeast extract | 2.0 G_PER_L |
FOODON:03315426
yeast extract
|
From CCAP Medium MR_EG_JM.pdf; Curated from CCAP Medium MR_EG_JM.pdf specification |
| 5 | Add the above constituents and make up to 1 litre with deionised water. For agar, add | 15 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_EG_JM.pdf; Curated from CCAP Medium MR_EG_JM.pdf specification | |
| 6 |
Na EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.45 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
From CCAP Medium MR_EG_JM.pdf; Curated from CCAP Medium MR_EG_JM.pdf specification |
| 7 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.278 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
From CCAP Medium MR_EG_JM.pdf; Curated from CCAP Medium MR_EG_JM.pdf specification |
| 8 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.008 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
From CCAP Medium MR_EG_JM.pdf; Curated from CCAP Medium MR_EG_JM.pdf specification |
| 9 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.008 G_PER_L |
CHEBI:15956
biotin
|
From CCAP Medium MR_EG_JM.pdf; Curated from CCAP Medium MR_EG_JM.pdf specification |
| 10 | Make up to 1 litre with deionised water. For agar, add | 15.0 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_EG_JM.pdf; Curated from CCAP Medium MR_EG_JM.pdf specification |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- eg_jm (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: EG_JM, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_EG_JM.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.504319 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.082400Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000071 |
| 2026-03-17T19:45:04.036937+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "EG:JM" to "eg_jm" for consistent matching |
| 2026-04-04T20:18:55.128320 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.333129+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:56.521826+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-06T03:04:19.854644+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6b87e5ad6b1eb25d7acfa91130d68175f68ef3d65fe41d20c48f2c9677fcf8f7 |