Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_MWC.pdf
Composition
flowchart LR
CultureMech_000094["mwc"]
ing0_Na_EDTA["Na EDTA"]
CultureMech_000094 --> ing0_Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing0_Na_EDTA -.-> CHEBI_64734
ing1_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_000094 --> ing1_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing1_FeCl3_x_6_H2O -.-> CHEBI_86254
ing2_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000094 --> ing2_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing2_CuSO4_x_5_H2O -.-> CHEBI_31440
ing3_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000094 --> ing3_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing3_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing4_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000094 --> ing4_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing4_CoCl2_x_6_H2O -.-> CHEBI_53503
ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000094 --> ing5_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
ing6_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000094 --> ing6_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing6_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing7_H3BO3["H3BO3"]
CultureMech_000094 --> ing7_H3BO3
CHEBI_33118["CHEBI:33118"]
ing7_H3BO3 -.-> CHEBI_33118
ing8_Cyanocobalamin__Vitamin_B__["Cyanocobalamin (Vitamin B )"]
CultureMech_000094 --> ing8_Cyanocobalamin__Vitamin_B__
CHEBI_17439["CHEBI:17439"]
ing8_Cyanocobalamin__Vitamin_B__ -.-> CHEBI_17439
ing9_Biotin["Biotin"]
CultureMech_000094 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Thiamine_HCl__Vitamin_B__["Thiamine HCl (Vitamin B )"]
CultureMech_000094 --> ing10_Thiamine_HCl__Vitamin_B__
CHEBI_49105["CHEBI:49105"]
ing10_Thiamine_HCl__Vitamin_B__ -.-> CHEBI_49105
ing11_Thiamine_HCl__B____["Thiamine HCl (B ) -"]
CultureMech_000094 --> ing11_Thiamine_HCl__B____
ing11_Thiamine_HCl__B____ -.-> CHEBI_49105
ing12_Biotin["Biotin"]
CultureMech_000094 --> ing12_Biotin
ing12_Biotin -.-> CHEBI_15956
ing13_Cyanocobalamin__B__["Cyanocobalamin (B )"]
CultureMech_000094 --> ing13_Cyanocobalamin__B__
ing13_Cyanocobalamin__B__ -.-> CHEBI_17439
ing14_TES__Dry_Buffer_["TES (Dry Buffer)"]
CultureMech_000094 --> ing14_TES__Dry_Buffer_
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na EDTA
C10H14N2O8.2Na 336.208 g/mol
|
4.36 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
From CCAP Medium MR_MWC.pdf; Curated from CCAP Medium MR_MWC.pdf specification |
| 2 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
3.15 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
From CCAP Medium MR_MWC.pdf; Curated from CCAP Medium MR_MWC.pdf specification |
| 3 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
From CCAP Medium MR_MWC.pdf; Curated from CCAP Medium MR_MWC.pdf specification |
| 4 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.022 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
From CCAP Medium MR_MWC.pdf; Curated from CCAP Medium MR_MWC.pdf specification |
| 5 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.01 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
From CCAP Medium MR_MWC.pdf; Curated from CCAP Medium MR_MWC.pdf specification |
| 6 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.18 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
From CCAP Medium MR_MWC.pdf; Curated from CCAP Medium MR_MWC.pdf specification |
| 7 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.006 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
From CCAP Medium MR_MWC.pdf; Curated from CCAP Medium MR_MWC.pdf specification |
| 8 |
H3BO3
H3BO3 61.833 g/mol
|
1.00 G_PER_L |
CHEBI:33118
boric acid
|
From CCAP Medium MR_MWC.pdf; Curated from CCAP Medium MR_MWC.pdf specification Name repaired (#276): the source string `H BO` had lost its subscript digits; restored to `H3BO3` — grounded to boric acid (CHEBI:33118). |
| 9 |
Cyanocobalamin (Vitamin B )
C63H88CoN14O14P 1355.388 g/mol
|
0.0005 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
From CCAP Medium MR_MWC.pdf; Curated from CCAP Medium MR_MWC.pdf specification |
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.0005 G_PER_L |
CHEBI:15956
biotin
|
From CCAP Medium MR_MWC.pdf; Curated from CCAP Medium MR_MWC.pdf specification |
| 11 |
Thiamine HCl (Vitamin B )
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.1 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
From CCAP Medium MR_MWC.pdf; Curated from CCAP Medium MR_MWC.pdf specification |
| 12 |
Thiamine HCl (B ) -
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.1 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
From CCAP Medium MR_MWC.pdf; Curated from CCAP Medium MR_MWC.pdf specification |
| 13 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.1 G_PER_L |
CHEBI:15956
biotin
|
From CCAP Medium MR_MWC.pdf; Curated from CCAP Medium MR_MWC.pdf specification |
| 14 |
Cyanocobalamin (B )
C63H88CoN14O14P 1355.388 g/mol
|
0.1 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
From CCAP Medium MR_MWC.pdf; Curated from CCAP Medium MR_MWC.pdf specification |
| 15 | TES (Dry Buffer) | 0.115 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_MWC.pdf; Curated from CCAP Medium MR_MWC.pdf specification |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mwc (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: MWC, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_MWC.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.549158 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.144349Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000094 |
| 2026-03-17T19:45:04.099144+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MWC" to "mwc" for consistent matching |
| 2026-04-04T12:54:54.864895 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.560608+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:56.765437+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-06T03:04:20.167430+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2231a28a253c41b0d94e7c5de788284ac2f8005039d627669aa8f022d7cd3a28 |
| 2026-08-17T17:31:56.837514+00:00 | repair_subscript_stripped_names.py | REPAIRED_SUBSCRIPT_STRIPPED_NAME | Restored digits lost from formula ingredient name(s) by an upstream encoding step (#276): H BO -> H3BO3. The corrupted original is preserved in each ingredient's notes. |