sp (CultureMech:000135)

Overview

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_SP.pdf

Composition

flowchart LR
    CultureMech_000135["sp"]
    ing0_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000135 --> ing0_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing0_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing1_MnSO__7H_O["MnSO .7H O"]
    CultureMech_000135 --> ing1_MnSO__7H_O
    ing2_H3BO3["H3BO3"]
    CultureMech_000135 --> ing2_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing2_H3BO3 -.-> CHEBI_33118
    ing3_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000135 --> ing3_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing3_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing4_Co_NO3_2_6H2O["Co(NO3)2.6H2O"]
    CultureMech_000135 --> ing4_Co_NO3_2_6H2O
    CHEBI_86214["CHEBI:86214"]
    ing4_Co_NO3_2_6H2O -.-> CHEBI_86214
    ing5_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000135 --> ing5_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing5_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing6_FeSO4_x_7H2O["FeSO4 x 7H2O"]
    CultureMech_000135 --> ing6_FeSO4_x_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7H2O -.-> CHEBI_75836
    ing7_EDTANa["EDTANa"]
    CultureMech_000135 --> ing7_EDTANa
    CHEBI_64734["CHEBI:64734"]
    ing7_EDTANa -.-> CHEBI_64734
    ing8_Biotin["Biotin"]
    CultureMech_000135 --> ing8_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing8_Biotin -.-> CHEBI_15956
    ing9_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000135 --> ing9_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing9_Calcium_pantothenate -.-> CHEBI_31345
    ing10_Cyanocobalamin["Cyanocobalamin"]
    CultureMech_000135 --> ing10_Cyanocobalamin
    CHEBI_17439["CHEBI:17439"]
    ing10_Cyanocobalamin -.-> CHEBI_17439
    ing11_Folic_acid["Folic acid"]
    CultureMech_000135 --> ing11_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing11_Folic_acid -.-> CHEBI_27470
    ing12_Inositol["Inositol"]
    CultureMech_000135 --> ing12_Inositol
    CHEBI_17268["CHEBI:17268"]
    ing12_Inositol -.-> CHEBI_17268
    ing13_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000135 --> ing13_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing13_Nicotinic_acid -.-> CHEBI_15940
    ing14_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000135 --> ing14_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing14_Thiamine_HCl -.-> CHEBI_49105
    ing15_Thymine["Thymine"]
    CultureMech_000135 --> ing15_Thymine
    CHEBI_17821["CHEBI:17821"]
    ing15_Thymine -.-> CHEBI_17821
    ing16_NaHCO["NaHCO"]
    CultureMech_000135 --> ing16_NaHCO
    CHEBI_32139["CHEBI:32139"]
    ing16_NaHCO -.-> CHEBI_32139
    ing17_Na2CO3["Na2CO3"]
    CultureMech_000135 --> ing17_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing17_Na2CO3 -.-> CHEBI_29377
    ing18_K2HPO4["K2HPO4"]
    CultureMech_000135 --> ing18_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing18_K2HPO4 -.-> CHEBI_131527
    ing19_K2SO4["K2SO4"]
    CultureMech_000135 --> ing19_K2SO4
    ing20_NaCl["NaCl"]
    CultureMech_000135 --> ing20_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing20_NaCl -.-> CHEBI_26710
    ing21_MgSO__7H_O["MgSO .7H O"]
    CultureMech_000135 --> ing21_MgSO__7H_O
    CHEBI_31795["CHEBI:31795"]
    ing21_MgSO__7H_O -.-> CHEBI_31795
    ing22_FeSO4_x_7H2O["FeSO4 x 7H2O"]
    CultureMech_000135 --> ing22_FeSO4_x_7H2O
    ing22_FeSO4_x_7H2O -.-> CHEBI_75836
    ing23_EDTANa["EDTANa"]
    CultureMech_000135 --> ing23_EDTANa
    ing23_EDTANa -.-> CHEBI_64734

Ingredients (24)

#	Ingredient	Concentration	Ontology	Notes
1	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.001 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
2	MnSO .7H O	0.002 G_PER_L		Ontology mapping not yet available; From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
3	H3BO3 H3BO3 61.833 g/mol	0.010 G_PER_L	CHEBI:33118 boric acid	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification Name repaired (#276): the source string `H BO` had lost its subscript digits; restored to `H3BO3` — grounded to boric acid (CHEBI:33118).
4	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.001 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
5	Co(NO3)2.6H2O Co.6H2O.2NO3 291.031 g/mol	0.001 G_PER_L	CHEBI:86214 cobalt dinitrate hexahydrate	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification Name repaired (#276): the source string `Co(NO ) .6H O` had lost its subscript digits; restored to `Co(NO3)2.6H2O` — grounded to cobalt dinitrate hexahydrate.
6	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	0.00005 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
7	FeSO4 x 7H2O Fe.7H2O.O4S 278.013 g/mol	0.70 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
8	EDTANa C10H14N2O8.2Na 336.208 g/mol	0.80 G_PER_L	CHEBI:64734 EDTA disodium salt (anhydrous)	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
9	Biotin C10H16N2O3S 244.316 g/mol	0.0002 G_PER_L	CHEBI:15956 biotin	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
10	Calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.02 G_PER_L	CHEBI:31345 Calcium pantothenate	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
11	Cyanocobalamin C63H88CoN14O14P 1355.388 g/mol	0.004 G_PER_L	CHEBI:17439 cyanocob(III)alamin	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
12	Folic acid C19H19N7O6 441.404 g/mol	0.0004 G_PER_L	CHEBI:27470 folic acid	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
13	Inositol C6H12O6 180.156 g/mol	1.00 G_PER_L	CHEBI:17268 myo-inositol	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
14	Nicotinic acid C6H5NO2 123.111 g/mol	0.02 G_PER_L	CHEBI:15940 nicotinic acid	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
15	Thiamine HCl C12H17N4OS.Cl.HCl 337.276 g/mol	0.10 G_PER_L	CHEBI:49105 thiamine hydrochloride	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
16	Thymine C5H6N2O2 126.115 g/mol	0.60 G_PER_L	CHEBI:17821 thymine	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
17	NaHCO CHO3.Na 84.006 g/mol	27.22 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
18	Na2CO3 CO3.2Na 105.988 g/mol	8.06 G_PER_L	CHEBI:29377 sodium carbonate	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
19	K2HPO4 HO4P.2K 174.174 g/mol	1.00 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
20	K2SO4	2.00 G_PER_L		Ontology mapping not yet available; From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification Name repaired (#276): the source string `K SO` had lost its subscript digits; restored to `K2SO4` — potassium sulfate.
21	NaCl Cl.Na 58.443 g/mol	2.00 G_PER_L	CHEBI:26710 sodium chloride	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
22	MgSO .7H O 7H2O.Mg.O4S 246.473 g/mol	0.40 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
23	FeSO4 x 7H2O Fe.7H2O.O4S 278.013 g/mol	0.02 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification
24	EDTANa C10H14N2O8.2Na 336.208 g/mol	0.16 G_PER_L	CHEBI:64734 EDTA disodium salt (anhydrous)	From CCAP Medium MR_SP.pdf; Curated from CCAP Medium MR_SP.pdf specification

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

sp (normalized source unresolved)

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	ccap-import	Imported from CCAP Culture Collection	Source ID: SP, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_SP.pdf
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added placeholder ingredients for missing composition
2026-02-03T11:26:43.626339	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.255741Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000135
2026-03-17T19:45:04.204106+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "SP" to "sp" for consistent matching
2026-04-04T12:54:55.116143	batch_process_collection_media	EXPANDED_INGREDIENTS
2026-05-16T08:57:02.801754+00:00	migrate_data_quality_flags.py	MIGRATED_DATA_QUALITY_FLAGS	dict(4 keys) -> list(3 entries)
2026-05-16T09:02:57.032837+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-06-10T06:57:11.621516+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:37:34.007672+00:00	primary-term-regrounding-v1.0	Re-grounded wrong primary term id (OAK-verified)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:20.362205+00:00	merge_recipes.py	MERGED_RECIPES	merged 1 source recipe(s) on fingerprint da24f11559604c8ac734772f3a0ab042644d81dd05b29556048bbca89aaa912d
2026-08-17T17:31:57.235595+00:00	repair_subscript_stripped_names.py	REPAIRED_SUBSCRIPT_STRIPPED_NAME	Restored digits lost from formula ingredient name(s) by an upstream encoding step (#276): H BO -> H3BO3; Co(NO ) .6H O -> Co(NO3)2.6H2O; K SO -> K2SO4. The corrupted original is preserved in each ingredient's notes.