Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_Waris_H.pdf
Composition
flowchart LR
CultureMech_000143["waris_h"]
ing0_Dissolve["Dissolve"]
CultureMech_000143 --> ing0_Dissolve
ing1_Dissolve["Dissolve"]
CultureMech_000143 --> ing1_Dissolve
ing2_KOH["KOH"]
CultureMech_000143 --> ing2_KOH
CHEBI_32035["CHEBI:32035"]
ing2_KOH -.-> CHEBI_32035
ing3_Na_EDTA_2H2O["Na EDTA.2H2O"]
CultureMech_000143 --> ing3_Na_EDTA_2H2O
ing4_FeSO4_x_7H2O["FeSO4 x 7H2O"]
CultureMech_000143 --> ing4_FeSO4_x_7H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_x_7H2O -.-> CHEBI_75836
ing5_Na_EDTA["Na EDTA"]
CultureMech_000143 --> ing5_Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing5_Na_EDTA -.-> CHEBI_64734
ing6_H3BO3["H3BO3"]
CultureMech_000143 --> ing6_H3BO3
CHEBI_33118["CHEBI:33118"]
ing6_H3BO3 -.-> CHEBI_33118
ing7_MnSO4_H2O["MnSO4.H2O"]
CultureMech_000143 --> ing7_MnSO4_H2O
ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000143 --> ing8_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing9_CoSO__7H2O["CoSO .7H2O"]
CultureMech_000143 --> ing9_CoSO__7H2O
ing10_Thiamine_HCl["Thiamine HCl"]
CultureMech_000143 --> ing10_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing10_Thiamine_HCl -.-> CHEBI_49105
ing11_Biotin["Biotin"]
CultureMech_000143 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_Cyanocobalamin["Cyanocobalamin"]
CultureMech_000143 --> ing12_Cyanocobalamin
CHEBI_17439["CHEBI:17439"]
ing12_Cyanocobalamin -.-> CHEBI_17439
ing13_Niacinamide["Niacinamide"]
CultureMech_000143 --> ing13_Niacinamide
CHEBI_17154["CHEBI:17154"]
ing13_Niacinamide -.-> CHEBI_17154
ing14_Biotin["Biotin"]
CultureMech_000143 --> ing14_Biotin
ing14_Biotin -.-> CHEBI_15956
ing15_Cyanocobalamin["Cyanocobalamin"]
CultureMech_000143 --> ing15_Cyanocobalamin
ing15_Cyanocobalamin -.-> CHEBI_17439
ing16_Niacin["Niacin"]
CultureMech_000143 --> ing16_Niacin
CHEBI_15940["CHEBI:15940"]
ing16_Niacin -.-> CHEBI_15940
ing17_Make_up_to_1_litre_with_deionised_water_and_adjust_pH_to_7_0_with["Make up to 1 litre with deionised water and adjust pH to 7.0 with"]
CultureMech_000143 --> ing17_Make_up_to_1_litre_with_deionised_water_and_adjust_pH_to_7_0_with
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Dissolve | 1.04 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification | |
| 2 | Dissolve | 2.74 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification | |
| 3 |
KOH
HO.K 56.105 g/mol
|
1.04 G_PER_L |
CHEBI:32035
potassium hydroxide
|
From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification |
| 4 | Na EDTA.2H2O | 4.08 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification | |
| 5 |
FeSO4 x 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
2.74 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification |
| 6 |
Na EDTA
C10H14N2O8.2Na 336.208 g/mol
|
1.0 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification |
| 7 |
H3BO3
H3BO3 61.833 g/mol
|
1.12 G_PER_L |
CHEBI:33118
boric acid
|
From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification Name repaired (#276): the source string `H BO` had lost its subscript digits; restored to `H3BO3` — grounded to boric acid (CHEBI:33118). |
| 8 | MnSO4.H2O | 0.12 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification Name repaired (#276): the source string `MnSO .H O` had lost its subscript digits; restored to `MnSO4.H2O` — manganese(II) sulfate monohydrate. | |
| 9 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.022 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification |
| 10 | CoSO .7H2O | 0.005 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification | |
| 11 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.01 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification |
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.0001 G_PER_L |
CHEBI:15956
biotin
|
From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification |
| 13 |
Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
|
0.00002 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification |
| 14 |
Niacinamide
C6H6N2O 122.127 g/mol
|
0.00001 G_PER_L |
CHEBI:17154
nicotinamide
|
Ontology mapping not yet available; From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification |
| 15 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.01 G_PER_L |
CHEBI:15956
biotin
|
From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification |
| 16 |
Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
|
0.002 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification |
| 17 |
Niacin
C6H5NO2 123.111 g/mol
|
0.001 G_PER_L |
CHEBI:15940
nicotinic acid
|
From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification |
| 18 | Make up to 1 litre with deionised water and adjust pH to 7.0 with | 1 MOLAR | Ontology mapping not yet available; From CCAP Medium MR_Waris_H.pdf; Curated from CCAP Medium MR_Waris_H.pdf specification |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- waris_h (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: Waris_H, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_Waris_H.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.641321 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.278064Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000143 |
| 2026-03-17T19:45:04.224980+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Waris-H" to "waris_h" for consistent matching |
| 2026-04-04T12:54:55.039363 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.880667+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:57.119928+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-06T03:04:20.435199+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint fa04bcf9277f55284556b2d583a9a95130dcb89673f3c2fedcf1af8e7125f108 |
| 2026-08-17T17:32:00.189565+00:00 | repair_subscript_stripped_names.py | REPAIRED_SUBSCRIPT_STRIPPED_NAME | Restored digits lost from formula ingredient name(s) by an upstream encoding step (#276): H BO -> H3BO3; MnSO .H O -> MnSO4.H2O. The corrupted original is preserved in each ingredient's notes. |