f_2_medium

Overview

Applications

• Algae cultivation
• Phytoplankton culture
• Microalgae research

Synonyms

• {'name': '8_ppt_f_2_medium', 'source': 'unknown', 'original_category': 'algae'}

Composition

flowchart LR
    CultureMech_000170["f_2_medium"]
    ing0_1["1"]
    CultureMech_000170 --> ing0_1
    ing1_2["2"]
    CultureMech_000170 --> ing1_2
    ing2_3["3"]
    CultureMech_000170 --> ing2_3
    ing3_4["4"]
    CultureMech_000170 --> ing3_4
    ing4_5["5"]
    CultureMech_000170 --> ing4_5
    ing5_6["6"]
    CultureMech_000170 --> ing5_6
    ing6_7["7"]
    CultureMech_000170 --> ing6_7

Ingredients (7)

Preparation

1. Important Notes To Consider: The quality of water, including natural seawater, is important.
2. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc.
3. Natural seawater and natural freshwater should be obtained from a non-polluted source. When dissolving chemicals, wait for the first component to dissolve before adding the second.
4. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently.
5. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts. Attention should be given to the pH of the final medium.
6. In most cases, if pH adjustment is required, this occurs before sterilization.
7. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers.
8. This would make the medium very alkaline soon after removal from the autoclave.
9. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

• Preferred term

  Tetraselmis marina

  Term

  Id

  NCBITaxon:41888

  Label

  Tetraselmis marina

  Growth metrics

  • Measurement conditions

    F/2-medium for seven days in the first stage.

    Evidence

    • Reference

      PMID:28456040

      Supports

      SUPPORT

      Snippet

      When the cells were grown in F/2-medium for seven days in the first stage, the carotenoid and lipid contents, and productivity were 44g/kg (DW), 27% and 31mg/L/d, respectively.

      Explanation

      Literature evidence states that Tetraselmis marina cells were grown in F/2 medium for seven days.

    Is max attainment

    False

  • Measurement conditions

    F/2 first stage followed by three-day N-replete second stage with 4.41 mM NaNO3.

    Evidence

    • Reference

      PMID:28456040

      Supports

      SUPPORT

      Snippet

      After second stage of cultivation of T. marina for further 3-days under N-replete condition (4.41mM NaNO3) increased biomass concentration of 1900mg/L and lipid content of 50% were observed

      Explanation

      Literature evidence reports increased biomass after an F/2-derived nitrogen-replete second-stage variant.

    Is max attainment

    False

• Preferred term

  Phaeodactylum tricornutum

  Term

  Id

  NCBITaxon:556484

  Label

  Phaeodactylum tricornutum CCAP 1055/1

  Strain

  UTEX 642

  Growth metrics

  • Measurement conditions

    F/2ASW at 20 deg C, continuous illumination of 30-75 umol photons m-2 s-1, and aeration with ambient air or 1% CO2; reported mid-log harvest OD730 0.2-0.3.

    Evidence

    • Reference

      DOI:10.1104/pp.18.00453

      Supports

      SUPPORT

      Explanation

      Matsui et al. explicitly report Phaeodactylum tricornutum UTEX 642 cultivation in F/2ASW.

    Is max attainment

    False

  Evidence

  • Reference

    DOI:10.1104/pp.18.00453

    Supports

    SUPPORT

    Explanation

    The publication supports the organism-medium relationship for the F/2ASW variant of this parent F/2 medium record.

• Preferred term

  Thalassiosira pseudonana

  Term

  Id

  NCBITaxon:296543

  Label

  Thalassiosira pseudonana CCMP1335

  Strain

  CCMP1335

  Growth metrics

  • Measurement conditions

    F/2ASW at 20 deg C, continuous illumination of 30-75 umol photons m-2 s-1, and aeration with ambient air or 1% CO2; reported mid-log harvest OD730 0.1-0.15.

    Evidence

    • Reference

      DOI:10.1104/pp.18.00453

      Supports

      SUPPORT

      Explanation

      Matsui et al. explicitly report Thalassiosira pseudonana CCMP1335 cultivation in F/2ASW.

    Is max attainment

    False

  Evidence

  • Reference

    DOI:10.1104/pp.18.00453

    Supports

    SUPPORT

    Explanation

    The source supports growth on the F/2ASW variant for the same strain represented by NCBI Taxonomy ID 296543 and reference assembly GCF_000149405.2.

• Preferred term

  Chaetoceros gracilis

  Strain

  UTEX LB2658

  Growth metrics

  • Measurement conditions

    Modified F/2ASW supplemented with sodium selenite and sodium metasilicate; sodium concentration adjusted by NaCl addition and CO2 availability varied.

    Evidence

    • Reference

      DOI:10.1007/s10126-021-10037-4

      Supports

      SUPPORT

      Explanation

      Tsuji et al. report growth and CO2-concentrating mechanism experiments for Chaetoceros gracilis UTEX LB2658 using modified F/2ASW.

    Is max attainment

    False

  Evidence

  • Reference

    DOI:10.1007/s10126-021-10037-4

    Supports

    SUPPORT

    Explanation

    The study identifies the UTEX LB2658 strain and modified F/2ASW growth conditions; the UTEX strain page separately lists this strain as a liquid-culture Chaetoceros gracilis accession.

Recipe variants

• Name

  n_replete_second_stage_tetraselmis_marina

  Description

  F/2 culture followed by nitrogen-replete second-stage cultivation for enhanced biomass and lipid productivity of Tetraselmis marina.

  Modifications

  • Grow cells in F/2 medium for seven days as the first stage.
  • Shift to a nitrogen-replete second stage with 4.41 mM NaNO3 for three days.

  Purpose

  Capture a study-specific F/2-derived nitrogen repletion variant used to increase Tetraselmis marina biomass and lipid productivity.

  Evidence

  • Reference

    PMID:28456040

    Supports

    SUPPORT

    Snippet

    After second stage of cultivation of T. marina for further 3-days under N-replete condition (4.41mM NaNO3) increased biomass concentration of 1900mg/L and lipid content of 50% were observed

    Explanation

    Literature evidence describes a nitrogen-replete second-stage variant after initial F/2 cultivation.

• Name

  f_2asw_diatom_culture

  Description

  F/2 artificial seawater culture conditions used for marine diatom maintenance and transformation studies.

  Modifications

  • Use artificial seawater supplemented with half-strength Guillard f solution.
  • Maintain cultures at 20 deg C under continuous illumination at 30-75 umol photons m-2 s-1.
  • Aerate cultures with ambient air or 1% CO2 depending on the experiment.

  Purpose

  Represent F/2ASW as a study-specific F/2 medium implementation for marine diatoms.

  Evidence

  • Reference

    DOI:10.1104/pp.18.00453

    Supports

    SUPPORT

    Explanation

    Matsui et al. define F/2ASW as artificial seawater plus half-strength Guillard f solution and report use with Phaeodactylum tricornutum UTEX 642 and Thalassiosira pseudonana CCMP1335.

• Name

  modified_f_2asw_sodium_response

  Description

  Modified F/2 artificial seawater used for sodium and CO2 response experiments with Chaetoceros gracilis.

  Modifications

  • Supplement F/2ASW with 10 nM sodium selenite and 0.1 mM sodium metasilicate.
  • Adjust sodium concentration by altering NaCl addition to the basal medium.
  • Vary CO2 availability to distinguish sodium-dependent growth effects.

  Purpose

  Capture an experimental F/2ASW variant used to study carbon concentrating mechanism physiology.

  Evidence

  • Reference

    DOI:10.1007/s10126-021-10037-4

    Supports

    SUPPORT

    Explanation

    Tsuji et al. report Chaetoceros gracilis UTEX LB2658 growth experiments using a modified F/2ASW formulation.

Provenance

Merged from 2 source records.

• 8_ppt_F_2_Medium (CultureMech:000021)
• F_2_Medium (CultureMech:000170)