Overview
- External ontology
- mediadive.medium:1125 (source link unavailable) — HALOPHILIC MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.5
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1125.pdf
Composition
flowchart LR
CultureMech_000247["halophilic_medium"]
ing0_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000247 --> ing0_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing0_MgCl2_x_6_H2O -.-> CHEBI_86345
ing1_K2SO4["K2SO4"]
CultureMech_000247 --> ing1_K2SO4
CHEBI_32036["CHEBI:32036"]
ing1_K2SO4 -.-> CHEBI_32036
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000247 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_Yeast_extract["Yeast extract"]
CultureMech_000247 --> ing3_Yeast_extract
ing4_NH4Cl["NH4Cl"]
CultureMech_000247 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_KH2PO4["KH2PO4"]
CultureMech_000247 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_Sucrose["Sucrose"]
CultureMech_000247 --> ing6_Sucrose
CHEBI_17992["CHEBI:17992"]
ing6_Sucrose -.-> CHEBI_17992
ing7_NaCl["NaCl"]
CultureMech_000247 --> ing7_NaCl
CHEBI_26710["CHEBI:26710"]
ing7_NaCl -.-> CHEBI_26710
ing8_HEPES["HEPES"]
CultureMech_000247 --> ing8_HEPES
CHEBI_46756["CHEBI:46756"]
ing8_HEPES -.-> CHEBI_46756
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
20 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 2 |
K2SO4
2K.O4S 174.259 g/mol
|
5 G_PER_L |
CHEBI:32036
potassium sulfate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 | Yeast extract | 0.1 G_PER_L | ||
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.05 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
Sucrose
C12H22O11 342.297 g/mol
|
0.5 G_PER_L |
CHEBI:17992
sucrose
|
|
| 8 |
NaCl
Cl.Na 58.443 g/mol
|
180 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 9 | HEPES | 6 G_PER_L |
CHEBI:46756
HEPES
|
Preparation
- Adjust to pH 6.5
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- halophilic_medium (normalized source unresolved)
- halophilic_medium (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.453958Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1125 |
| 2026-03-13T06:18:53.754479Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000247 |
| 2026-03-17T17:06:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:04.659603+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOPHILIC MEDIUM" to "halophilic_medium" for consistent matching |
| 2026-05-16T09:02:57.287891+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-06-05T07:40:14.559108+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.188204+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:28.856598+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: halophilic_medium, halophilic_medium |
| 2026-08-06T03:04:28.856635+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 8e91af80b815b0264900951b5b8be1df331069336a9e52d693716c26b520a676 |