Overview
- External ontology
- mediadive.medium:1156 (source link unavailable) — HALOPHILIC METHANOTROPHIC BACTERIUM MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'halophilic_methanotrophic_bacterium_medium_replace_methane_with_methanol', 'source': 'KOMODO', 'source_id': 'komodo.medium:1156_replace_methane_with_Methanol', 'original_category': 'bacterial'}
- pH range
- {'min': 6.5, 'max': 7.5}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1156.pdf
Composition
flowchart LR
CultureMech_000248["halophilic_methanotrophic_bacterium_medium"]
ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000248 --> ing0_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
ing1_NH4Cl["NH4Cl"]
CultureMech_000248 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_000248 --> ing2_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing2_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing3_KH2PO4["KH2PO4"]
CultureMech_000248 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NaCl["NaCl"]
CultureMech_000248 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
CultureMech_000248 --> ing5_CaCl2_x_6_H2O
CHEBI_91243["CHEBI:91243"]
ing5_CaCl2_x_6_H2O -.-> CHEBI_91243
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000248 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000248 --> ing7_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing8_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000248 --> ing8_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing8_CuSO4_x_5_H2O -.-> CHEBI_31440
ing9_MnSO4_x_2_H2O["MnSO4 x 2 H2O"]
CultureMech_000248 --> ing9_MnSO4_x_2_H2O
CHEBI_86356["CHEBI:86356"]
ing9_MnSO4_x_2_H2O -.-> CHEBI_86356
ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000248 --> ing10_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing11_H3BO3["H3BO3"]
CultureMech_000248 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000248 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.7 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
65 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
|
0.01 G_PER_L |
CHEBI:91243
calcium chloride hexahydrate
|
|
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.005 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 8 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.44 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 9 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.2 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 10 |
MnSO4 x 2 H2O
2H2O.Mn.O4S 187.031 g/mol
|
0.17 G_PER_L |
CHEBI:86356
manganese(II) sulfate dihydrate
|
|
| 11 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.06 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.08 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Preparation
- pH-value 7.2 (Adjust to 7.6 with 10% NaOH before sterilization)
- Culture bottles: 120-ml Serum bottles with 25 ml medium closed with butyl rubber serum caps and aluminium crimps
- Gas supply: 2 vol.% CO2; 20 vol.% CH4; 78 vol.% air (Alternate cultivation method: instead of a methane-containing headspace, add 0.5% (w/v) methanol to the medium)
- Culture conditions: 30°C; shaking culture on rotary shaker (200 rpm)
- Notes: pH is critical, final pH must be between 6.5-7.5; some insoluble phosphates may precipitate during medium preparation (should not be more than OD 0.005).
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- DSMZ_1156_HALOPHILIC_METHANOTROPHIC_BACTERIUM_MEDIUM (CultureMech:000248)
- halophilic_methanotrophic_bacterium_medium (CultureMech:003866)
- halophilic_methanotrophic_bacterium_medium_replace_methane_with_methanol (CultureMech:003865)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.535182Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1156 |
| 2026-03-13T06:18:53.761145Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000248 |
| 2026-03-17T17:06:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:04.667162+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOPHILIC METHANOTROPHIC BACTERIUM MEDIUM" to "halophilic_methanotrophic_bacterium_medium" for consistent matching |
| 2026-05-16T09:02:57.295683+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-05-20T06:00:46.024745+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.5-7.5' -> {'min': 6.5, 'max': 7.5} |
| 2026-06-05T07:40:14.567360+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.190856+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:34.349959+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: DSMZ_1156_HALOPHILIC_METHANOTROPHIC_BACTERIUM_MEDIUM, halophilic_methanotrophic_bacterium_medium, halophilic_methanotrophic_bacterium_medium_replace_methane_with_methanol |
| 2026-08-06T03:04:34.350008+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint effb076e47110fdc9d54203f3d1a0051936557b131fc071768cc70d0b3011b41 |