alkalophilic_halophile_medium

CultureMech:000255 archaea COMPLEX SOLID_AGAR
Original name: ALKALOPHILIC HALOPHILE MEDIUM

Overview

External ontology
mediadive.medium:1431 (source link unavailable) — ALKALOPHILIC HALOPHILE MEDIUM
Applications
  • Microbial cultivation
pH
9.0
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1431.pdf

Composition

flowchart LR
    CultureMech_000255["alkalophilic_halophile_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_000255 --> ing0_Yeast_extract
    ing1_Casamino_acids["Casamino acids"]
    CultureMech_000255 --> ing1_Casamino_acids
    ing2_Trisodium_citrate["Trisodium citrate"]
    CultureMech_000255 --> ing2_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing2_Trisodium_citrate -.-> CHEBI_53258
    ing3_KCl["KCl"]
    CultureMech_000255 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000255 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000255 --> ing5_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000255 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_NaCl["NaCl"]
    CultureMech_000255 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710
    ing8_Na2CO3["Na2CO3"]
    CultureMech_000255 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377
    ing9_Agar["Agar"]
    CultureMech_000255 --> ing9_Agar
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar -.-> CHEBI_2509

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Yeast extract 10 G_PER_L
2 Casamino acids 7.5 G_PER_L
3 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
4 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.00036 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
7 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.05 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
8 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride
9 Na2CO3
CO3.2Na 105.988 g/mol
50 G_PER_L CHEBI:29377
sodium carbonate
10 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. Liquid medium may be prepared by adding all components together. Agar (2%) medium should be prepared without Na2CO3 and sterilised by autoclaving. Add 20-30 ml of a sterile 10% Na2CO3 solution to the autoclaved medium containing agar so that the final pH is 9.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:14.401050Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1431
2026-01-28T19:48:44.555973Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:53.815164Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000255
2026-03-17T17:06:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:04.725995+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ALKALOPHILIC HALOPHILE MEDIUM" to "alkalophilic_halophile_medium" for consistent matching
2026-05-16T09:02:57.215762+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS category->lowercase=1
2026-06-05T07:40:14.480262+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:27.100005+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:42.157656+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:28.227104+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: alkalophilic_halophile_medium, alkalophilic_halophile_medium
2026-08-06T03:04:28.227153+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 8bac7522eda9372c0bf9616988ee794474df666ede3f6d0a68b13f1419fbc0b2