methanohalophilus_portucalensis_medium

Overview

External ontology

mediadive.medium:348 (source link unavailable) — METHANOHALOPHILUS PORTUCALENSIS MEDIUM

Applications

• Microbial cultivation

Synonyms

• {'name': 'sf1_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:348', 'original_category': 'bacterial'}

pH range

{'min': 7.3, 'max': 7.4}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium348.pdf

Composition

flowchart LR
    CultureMech_000262["methanohalophilus_portucalensis_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000262 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000262 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000262 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000262 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_000262 --> ing4_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing5_NH4Cl["NH4Cl"]
    CultureMech_000262 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_KCl["KCl"]
    CultureMech_000262 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_000262 --> ing7_Yeast_extract
    ing8_Trypticase_peptone["Trypticase peptone"]
    CultureMech_000262 --> ing8_Trypticase_peptone
    ing9_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000262 --> ing9_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Sodium_resazurin -.-> CHEBI_8806
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000262 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Na2CO3["Na2CO3"]
    CultureMech_000262 --> ing11_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing11_Na2CO3 -.-> CHEBI_29377
    ing12_Trimethylamine_HCl["Trimethylamine-HCl"]
    CultureMech_000262 --> ing12_Trimethylamine_HCl
    CHEBI_64700["CHEBI:64700"]
    ing12_Trimethylamine_HCl -.-> CHEBI_64700
    ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000262 --> ing13_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing13_Na2S_x_9_H2O -.-> CHEBI_76209
    ing14_HCl["HCl"]
    CultureMech_000262 --> ing14_HCl
    CHEBI_17883["CHEBI:17883"]
    ing14_HCl -.-> CHEBI_17883
    ing15_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000262 --> ing15_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing15_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing16_ZnCl2["ZnCl2"]
    CultureMech_000262 --> ing16_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing16_ZnCl2 -.-> CHEBI_49976
    ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000262 --> ing17_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing18_H3BO3["H3BO3"]
    CultureMech_000262 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000262 --> ing19_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000262 --> ing20_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000262 --> ing21_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000262 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing23_NaOH["NaOH"]
    CultureMech_000262 --> ing23_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing23_NaOH -.-> CHEBI_32145
    ing24_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000262 --> ing24_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing24_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing25_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000262 --> ing25_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing25_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (26)

Preparation

1. Dissolve ingredients except cysteine, carbonate, trimethylamine and sulfide, bring medium to the boil, then cool to room temperature while sparging with 80% N2 and 20% CO2 gas mixture. Add cysteine and adjust pH to 6.5, then dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add trimethylamine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 7.3 - 7.4, if necessary.
2. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

• methanohalophilus_portucalensis_medium (CultureMech:000262)
• sf1_medium (CultureMech:005061)