Overview
- External ontology
- mediadive.medium:479a (source link unavailable) — METHANOHALOPHILUS PROFUNDI MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium479a.pdf
Composition
flowchart LR
CultureMech_000267["methanohalophilus_profundi_medium"]
ing0_NaCl["NaCl"]
CultureMech_000267 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_000267 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000267 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000267 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_000267 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_000267 --> ing5_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing6_Yeast_extract["Yeast extract"]
CultureMech_000267 --> ing6_Yeast_extract
ing7_Trypticase_peptone["Trypticase peptone"]
CultureMech_000267 --> ing7_Trypticase_peptone
ing8_HEPES["HEPES"]
CultureMech_000267 --> ing8_HEPES
CHEBI_46756["CHEBI:46756"]
ing8_HEPES -.-> CHEBI_46756
ing9_Sodium_resazurin["Sodium resazurin"]
CultureMech_000267 --> ing9_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Sodium_resazurin -.-> CHEBI_8806
ing10_Trimethylamine_HCl["Trimethylamine-HCl"]
CultureMech_000267 --> ing10_Trimethylamine_HCl
CHEBI_64700["CHEBI:64700"]
ing10_Trimethylamine_HCl -.-> CHEBI_64700
ing11_2_Mercaptoethanesulfonate["2-Mercaptoethanesulfonate"]
CultureMech_000267 --> ing11_2_Mercaptoethanesulfonate
CHEBI_17905["CHEBI:17905"]
ing11_2_Mercaptoethanesulfonate -.-> CHEBI_17905
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_000267 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_000267 --> ing13_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000267 --> ing14_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
ing15_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_000267 --> ing15_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing15_MnSO4_x_H2O -.-> CHEBI_86364
ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000267 --> ing16_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
ing17_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_000267 --> ing17_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing17_CoSO4_x_7_H2O -.-> CHEBI_91244
ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000267 --> ing18_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000267 --> ing19_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
ing20_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_000267 --> ing20_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing20_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing21_H3BO3["H3BO3"]
CultureMech_000267 --> ing21_H3BO3
CHEBI_33118["CHEBI:33118"]
ing21_H3BO3 -.-> CHEBI_33118
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000267 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000267 --> ing23_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
ing24_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_000267 --> ing24_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing24_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing25_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_000267 --> ing25_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing25_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (26)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
119.812 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 118.812, 1.0] |
| 2 |
KCl
Cl.K 74.551 g/mol
|
1.48515 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5.94059 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.49604000000000004 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.39604, 0.1] |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.990099 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
0.39604 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 | Yeast extract | 1.9802 G_PER_L | ||
| 8 | Trypticase peptone | 1.9802 G_PER_L | ||
| 9 | HEPES | 4.9505 G_PER_L |
CHEBI:46756
HEPES
|
|
| 10 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.00049505 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
Trimethylamine-HCl
C3H9N.HCl 95.573 g/mol
|
1.9802 G_PER_L |
CHEBI:64700
trimethylamine hydrochloride
|
|
| 12 |
2-Mercaptoethanesulfonate
C2H6O3S2 142.201 g/mol
|
0.19802 G_PER_L |
CHEBI:17905
coenzyme M
|
|
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.247525 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 14 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 15 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 16 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 17 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 18 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 19 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 20 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 21 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 22 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 24 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 25 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 26 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve ingredients except trimethylamine, coenzyme M and sulfide. Adjust pH to 7.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic; then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add trimethylamine, coenzyme M and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 7.5, if necessary.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methanohalophilus_profundi_medium (CultureMech:000267)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.298873Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 479a |
| 2026-01-28T19:48:44.647352Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:46.903247Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:18:53.948004Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000267 |
| 2026-03-17T17:06:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:04.871092+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOHALOPHILUS PROFUNDI MEDIUM" to "methanohalophilus_profundi_medium" for consistent matching |
| 2026-05-16T09:02:57.610356+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-06-05T07:40:14.898731+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.307948+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:30.567559+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f520830b04bd1aeb2c20bc8ce35c56d442bbbde8f646af4d55dc87aecec1c451 |