dichotomicrobium_thermohalophilum_medium

Overview

External ontology

mediadive.medium:590 (source link unavailable) — DICHOTOMICROBIUM THERMOHALOPHILUM MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 7.0, 'max': 7.2}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium590.pdf

Composition

flowchart LR
    CultureMech_000274["dichotomicrobium_thermohalophilum_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_000274 --> ing0_Yeast_extract
    ing1_Na2_DL_malate["Na2-DL-malate"]
    CultureMech_000274 --> ing1_Na2_DL_malate
    CHEBI_91260["CHEBI:91260"]
    ing1_Na2_DL_malate -.-> CHEBI_91260
    ing2_Agar["Agar"]
    CultureMech_000274 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_NaHCO3["NaHCO3"]
    CultureMech_000274 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_NaCl["NaCl"]
    CultureMech_000274 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Na2SO4["Na2SO4"]
    CultureMech_000274 --> ing5_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing5_Na2SO4 -.-> CHEBI_32149
    ing6_KCl["KCl"]
    CultureMech_000274 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_KBr["KBr"]
    CultureMech_000274 --> ing7_KBr
    CHEBI_32030["CHEBI:32030"]
    ing7_KBr -.-> CHEBI_32030
    ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000274 --> ing8_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000274 --> ing9_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing10_H3BO3["H3BO3"]
    CultureMech_000274 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000274 --> ing11_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000274 --> ing12_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing13__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_000274 --> ing13__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing13__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000274 --> ing14_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing15_Na_EDTA["Na-EDTA"]
    CultureMech_000274 --> ing15_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing15_Na_EDTA -.-> CHEBI_64734
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000274 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_000274 --> ing17_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing17_MnSO4_x_H2O -.-> CHEBI_86364
    ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000274 --> ing18_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing19_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_000274 --> ing19_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing19_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing20_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_000274 --> ing20_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing20_Na2B4O7_x_10_H2O -.-> CHEBI_131366

Ingredients (21)

Preparation

1. pH 7.0-7.2
2. Agar at a concentration of 18 g/l is added for solid media.
3. Prepare an 8% solution of NaHCO3 in half full 100ml serum bottles closed with a rubber septum and autoclave. Add 28.4 ml of the sterile 8% solution of NaHCO3 (2.822g/l) per litre of the sterile, cooled medium.
4. Add NaHCO3 from a filter-sterilized stock solution when the medium has cooled.
5. Dissolve the nitrilotriacetic acid.
6. Adjust pH to 7.0 with KOH (~7.3 g).
7. Dissolve other salts separately and combine.
8. Adjust pH to 6.8 with NaOH or H2SO4.
9. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

• DSMZ_590_DICHOTOMICROBIUM_THERMOHALOPHILUM_MEDIUM (CultureMech:000274)
• dichotomicrobium_thermohalophilum_medium (CultureMech:006070)