hydrogenobacter_halophilus_medium

CultureMech:000281 bacterial DEFINED LIQUID
Original name: HYDROGENOBACTER HALOPHILUS MEDIUM

Overview

External ontology
mediadive.medium:744 (source link unavailable) — HYDROGENOBACTER HALOPHILUS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 6.8, 'max': 7.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium744.pdf

Composition

flowchart LR
    CultureMech_000281["hydrogenobacter_halophilus_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_000281 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000281 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000281 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_NaCl["NaCl"]
    CultureMech_000281 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000281 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2["CaCl2"]
    CultureMech_000281 --> ing5_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing5_CaCl2 -.-> CHEBI_3312
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000281 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_MoO3["MoO3"]
    CultureMech_000281 --> ing7_MoO3
    CHEBI_30627["CHEBI:30627"]
    ing7_MoO3 -.-> CHEBI_30627
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000281 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000281 --> ing9_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing9_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing10_H3BO3["H3BO3"]
    CultureMech_000281 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_MnSO4_x_5_H2O["MnSO4 x 5 H2O"]
    CultureMech_000281 --> ing11_MnSO4_x_5_H2O
    CHEBI_131524["CHEBI:131524"]
    ing11_MnSO4_x_5_H2O -.-> CHEBI_131524
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000281 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2 G_PER_L CHEBI:62946
ammonium sulfate
4 NaCl
Cl.Na 58.443 g/mol
29.3 G_PER_L CHEBI:26710
sodium chloride
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaCl2
Ca.2Cl 110.984 g/mol
0.01 G_PER_L CHEBI:3312
calcium dichloride
7 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.01 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
8 MoO3
MoO3 143.937 g/mol
0.004 G_PER_L CHEBI:30627
molybdenum trioxide
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.028 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
10 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.002 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
11 H3BO3
H3BO3 61.833 g/mol
0.004 G_PER_L CHEBI:33118
boric acid
12 MnSO4 x 5 H2O
5H2O.Mn.O4S 241.076 g/mol
0.004 G_PER_L CHEBI:131524
manganese(II) sulfate pentahydrate
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.004 G_PER_L CHEBI:53503
cobalt chloride hexahydrate

Preparation

  1. Adjust pH to 6.8-7.0.
  2. Incubate at 37°C under a gas phase of H2:O2:CO2 = 8:1:1.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:12.117527Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 744
2026-03-13T06:18:54.087945Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000281
2026-03-17T17:06:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.025291+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HYDROGENOBACTER HALOPHILUS MEDIUM" to "hydrogenobacter_halophilus_medium" for consistent matching
2026-05-16T09:02:57.326746+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS category->lowercase=1
2026-05-20T06:00:46.049109+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0}
2026-06-05T07:40:14.600718+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.201717+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:34.842177+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: DSMZ_744_HYDROGENOBACTER_HALOPHILUS_MEDIUM, hydrogenobacter_halophilus_medium
2026-08-06T03:04:34.842220+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint dd109bc8543c0cde7e1f377f21b34e56653f441d0005ebc8447da3494ca63a24